Fast DNA-PAINT imaging using a deep neural network.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
27 08 2022
27 08 2022
Historique:
received:
08
12
2021
accepted:
09
08
2022
entrez:
27
8
2022
pubmed:
28
8
2022
medline:
31
8
2022
Statut:
epublish
Résumé
DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) is a super-resolution technique with relatively easy-to-implement multi-target imaging. However, image acquisition is slow as sufficient statistical data has to be generated from spatio-temporally isolated single emitters. Here, we train the neural network (NN) DeepSTORM to predict fluorophore positions from high emitter density DNA-PAINT data. This achieves image acquisition in one minute. We demonstrate multi-colour super-resolution imaging of structure-conserved semi-thin neuronal tissue and imaging of large samples. This improvement can be integrated into any single-molecule imaging modality to enable fast single-molecule super-resolution microscopy.
Identifiants
pubmed: 36030338
doi: 10.1038/s41467-022-32626-0
pii: 10.1038/s41467-022-32626-0
pmc: PMC9420107
doi:
Substances chimiques
Fluorescent Dyes
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5047Informations de copyright
© 2022. The Author(s).
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