Air-liquid organotypic assays to investigate cellular crosstalk in the tumor microenvironment of cancer cells.

Cell Line, Tumor Neoplasms Tumor Microenvironment
Biotechnology and bioengineering Cancer Cell Biology Cell culture Tissue Engineering

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
16 09 2022
Historique:
entrez: 29 8 2022
pubmed: 30 8 2022
medline: 31 8 2022
Statut: epublish

Résumé

Air-liquid organotypic culture models enable the study of the cellular crosstalk in the tumor microenvironment. This 3D assay recapitulates the tumor niche more faithfully than 2D culture systems and represents a versatile platform that can be easily adapted to different types of cancer cells, stromal components, or ECM composition. Here, we detail the steps to build an organotypic culture including the preparation of the organotypic structure, organotypic gels, cell seeding, gel casting, membrane processing, and image and data analysis. For complete details on the use and execution of this protocol, please refer to Linares et al. (2022).

Identifiants

DOI: 10.1016/j.xpro.2022.101635 PMID: 36035805 PMC: PMC9405995
pubmed: 36035805
doi: 10.1016/j.xpro.2022.101635
pii: S2666-1667(22)00515-9
pmc: PMC9405995
pii:
doi:

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

101635

Subventions

Organisme : NCI NIH HHS
ID : R50 CA265332
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA265892
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA218254
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA246765
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA250025
Pays : United States

Informations de copyright

© 2022 The Author(s).

Déclaration de conflit d'intérêts

The authors declare no competing interests.

Références

J Pathol. 2005 Mar;205(4):468-75
pubmed: 15685705
Nat Cell Biol. 2007 Dec;9(12):1392-400
pubmed: 18037882
Dev Cell. 2021 Jan 11;56(1):95-110.e10
pubmed: 33207226
Cell Rep. 2022 May 10;39(6):110792
pubmed: 35545049
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Cancer Cell. 2014 Jul 14;26(1):121-135
pubmed: 25002027

Auteurs

Tania Cid-Diaz (T)

Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA. Electronic address: tcd4002@med.cornell.edu.

Andrea C Lodeiro (AC)

Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA.

Angeles Duran (A)

Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA.

Jorge Moscat (J)

Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA.

Maria T Diaz-Meco (MT)

Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA. Electronic address: mtd4001@med.cornell.edu.

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Classifications MeSH

questionsmedicales.fr › Cellules › Cellules cultivées › Cellules cancéreuses en culture › Lignée cellulaire tumorale › Air-liquid organotypic assays to investigate...
questionsmedicales.fr › Tumeurs › Air-liquid organotypic assays to investigate...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Microenvironnement cellulaire › Microenvironnement tumoral › Air-liquid organotypic assays to investigate...