Investigation of α-Synuclein and Amyloid-β(42)-E22Δ Oligomers Using SiN Nanopore Functionalized with L-Dopa.
aggregation
amyloid-β
nanopore
single molecule
α-synuclein
Journal
Chemistry, an Asian journal
ISSN: 1861-471X
Titre abrégé: Chem Asian J
Pays: Germany
ID NLM: 101294643
Informations de publication
Date de publication:
17 Oct 2022
17 Oct 2022
Historique:
revised:
25
08
2022
received:
11
07
2022
pubmed:
30
8
2022
medline:
22
10
2022
entrez:
29
8
2022
Statut:
ppublish
Résumé
Solid-state nanopores are an emerging technology used as a high-throughput, label-free analytical method for the characterization of protein aggregation in an aqueous solution. In this work, we used Levodopamine to coat a silicon nitride nanopore surface that was fabricated through a dielectric breakdown in order to reduce the unspecific adsorption. The coating of inner nanopore wall by investigation of the translocation of heparin. The functionalized nanopore was used to investigate the aggregation of amyloid-β and α-synuclein, two biomarkers of degenerative diseases. In the first application, we demonstrate that the α-synuclein WT is more prone to form dimers than the variant A53T. In the second one, we show for the Aβ(42)-E22Δ (Osaka mutant) that the addition of Aβ(42)-WT monomers increases the polymorphism of oligomers, while the incubation with Aβ(42)-WT fibrils generates larger aggregates.
Identifiants
pubmed: 36038502
doi: 10.1002/asia.202200726
pmc: PMC9826174
doi:
Substances chimiques
alpha-Synuclein
0
Protein Aggregates
0
Levodopa
46627O600J
Amyloid beta-Peptides
0
Heparin
9005-49-6
Amyloid
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202200726Subventions
Organisme : Agence Nationale de la Recherche
ID : ANR-19-CE42-0006
Organisme : University of Franche-Comté
ID : 2021 - DARIA0110913048
Organisme : GENCI
Informations de copyright
© 2022 The Authors. Chemistry - An Asian Journal published by Wiley-VCH GmbH.
Références
Mol Cell Neurosci. 2016 Mar;71:92-101
pubmed: 26711807
Nanoscale. 2019 Mar 28;11(13):6480-6488
pubmed: 30892349
J Phys Chem Lett. 2022 Jun 30;13(25):5918-5924
pubmed: 35731125
Anal Chem. 2009 Dec 1;81(23):9689-94
pubmed: 19877660
Acta Pharmacol Sin. 2017 Sep;38(9):1205-1235
pubmed: 28713158
ACS Nano. 2018 May 22;12(5):4494-4502
pubmed: 29630824
Sci Rep. 2016 Feb 11;6:20776
pubmed: 26865505
J Chem Phys. 2018 Mar 28;148(12):123322
pubmed: 29604892
Nano Lett. 2005 Oct;5(10):1905-9
pubmed: 16218707
PLoS One. 2014 Mar 21;9(3):e92880
pubmed: 24658537
Analyst. 2012 Aug 21;137(16):3806-13
pubmed: 22768391
J Am Chem Soc. 2021 Oct 13;143(40):16621-16629
pubmed: 34582216
Chem Asian J. 2022 Oct 17;17(20):e202200726
pubmed: 36038502
Nat Nanotechnol. 2011 Apr;6(4):253-60
pubmed: 21336266
Sci Adv. 2019 Apr 17;5(4):eaau3112
pubmed: 31001578
Nat Protoc. 2020 Jan;15(1):122-143
pubmed: 31836867
Acta Neuropathol. 2017 Dec;134(6):819-838
pubmed: 28803412
Int J Mol Sci. 2017 Mar 02;18(3):
pubmed: 28257086
Biosens Bioelectron. 2021 Jul 1;183:113195
pubmed: 33857755
Appl Phys Lett. 2007 Jul 31;91(5):539011-539013
pubmed: 18392111
Chem Soc Rev. 2021 Apr 26;50(8):4974-4992
pubmed: 33623941
Nano Lett. 2013 Feb 13;13(2):658-63
pubmed: 23343345
J Phys Chem B. 2022 Mar 24;126(11):2217-2229
pubmed: 35276047
Protein Pept Lett. 2014 Mar;21(3):256-65
pubmed: 24370259
Biopolymers. 2016 Dec;105(12):905-13
pubmed: 27543274
J Biol Chem. 2005 Mar 11;280(10):9595-603
pubmed: 15615727
ACS Nano. 2017 Jun 27;11(6):5706-5716
pubmed: 28471644
ACS Nano. 2019 May 28;13(5):5231-5242
pubmed: 30995394
ACS Nano. 2012 Jul 24;6(7):5909-19
pubmed: 22686709
J Comput Chem. 2005 Dec;26(16):1781-802
pubmed: 16222654
Langmuir. 2018 Jul 31;34(30):8866-8874
pubmed: 30001624
J Phys Chem B. 1998 Apr 30;102(18):3586-616
pubmed: 24889800
ACS Cent Sci. 2022 Apr 27;8(4):441-448
pubmed: 35505874
Int J Mol Sci. 2020 Apr 17;21(8):
pubmed: 32316558
ACS Sens. 2021 Oct 22;6(10):3733-3743
pubmed: 34554735
Adv Healthc Mater. 2020 Sep;9(17):e2000933
pubmed: 32734703
ACS Appl Mater Interfaces. 2017 Dec 6;9(48):41634-41640
pubmed: 29144721
Anal Biochem. 2003 Jan 1;312(1):33-9
pubmed: 12479832
Langmuir. 2016 Sep 6;32(35):8916-25
pubmed: 27506271
Biomacromolecules. 2005 Jan-Feb;6(1):168-73
pubmed: 15638517
Chemosphere. 2022 Mar;291(Pt 1):132733
pubmed: 34742766
Methods Mol Biol. 2011;749:317-58
pubmed: 21674382
ACS Nano. 2017 Jul 25;11(7):7091-7100
pubmed: 28693322
Proc Natl Acad Sci U S A. 2016 Sep 27;113(39):10866-71
pubmed: 27621459
Nat Nanotechnol. 2017 May;12(4):360-367
pubmed: 27992411
Anal Chem. 2018 Dec 4;90(23):13826-13831
pubmed: 30406650
Nanomaterials (Basel). 2021 Jul 28;11(8):
pubmed: 34443773
Biophys J. 2014 Feb 4;106(3):696-704
pubmed: 24507610
ACS Appl Mater Interfaces. 2020 Mar 25;12(12):14563-14568
pubmed: 32129065