Influence of exogenous phytohormone supplementation on the pigment and fatty acid content of three marine diatoms.
Diatoms
EPA; Pigments; Fucoxanthin
Microalgae
PUFAs
Phytohormones
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
Sep 2022
Sep 2022
Historique:
received:
26
05
2022
accepted:
13
08
2022
revised:
09
08
2022
pubmed:
31
8
2022
medline:
15
9
2022
entrez:
30
8
2022
Statut:
ppublish
Résumé
Diatoms are ubiquitous photosynthetic microorganisms with great potential for biotechnological applications. However, their commercialisation is hampered by production costs, requiring hence optimisation of cultivation methods. Phytohormones are plant growth regulators which may be used to influence physiological processes in microalgae, including diatoms. In this study, the model species Phaeodactylum tricornutum (Phaeodactylaceae) and two Irish isolates of Stauroneis sp. (Stauroneidaceae) and Nitzschia sp. (Bacillariaceae) were grown with varying amounts of the phytohormones indoleacetic acid (IAA), gibberellic acid (GA3), methyl jasmonate (MJ), abscisic acid (ABA) or salicylic acid (SA), and their influence on pigment and fatty acid profiles was monitored. The application of GA3 (200 mg/l) stimulated the growth of P. tricornutum which accumulated 52% more dry biomass compared to the control and concomitantly returned the highest eicosapentaenoic acid (EPA) yield (0.6 mg/l). The highest fucoxanthin yield (0.18 mg/l) was obtained for P. tricornutum cultivated with GA3 (2 mg/l) supplementation. In Stauroneis sp., SA (1 mg/l) had the most positive effect on EPA, the content of which was enhanced up to 45.7 μg/mg (4.6% of total dry weight). The SA (1 mg/l) treatment also boosted carotenogenesis in Nitzschia sp., leading to 1.7- and 14-fold increases in fucoxanthin and β-carotene compared to the control, respectively. Of note, MJ (0.5 mg/l) increased the EPA content of all diatom species compared to their controls. These results indicate that phytohormone-based treatments can be used to alter the pigment and lipid content of microalgae, which tend to respond in dose- and species-specific manners to individual compounds.Key points• Response to phytohormones was investigated in diatoms from distinct families.• MJ (0.5 mg/l) caused an increase in EPA cellular content in all three diatoms.• Phytohormones mostly caused dose-dependent and species-specific responses.
Identifiants
pubmed: 36040486
doi: 10.1007/s00253-022-12140-5
pii: 10.1007/s00253-022-12140-5
doi:
Substances chimiques
Fatty Acids
0
Plant Growth Regulators
0
Eicosapentaenoic Acid
AAN7QOV9EA
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6195-6207Subventions
Organisme : VES4US
ID : 801338
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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