Membrane fission during bacterial spore development requires cellular inflation driven by DNA translocation.
FisB
endospore formation
membrane fission
membrane tension
Journal
Current biology : CB
ISSN: 1879-0445
Titre abrégé: Curr Biol
Pays: England
ID NLM: 9107782
Informations de publication
Date de publication:
10 10 2022
10 10 2022
Historique:
received:
15
11
2021
revised:
26
04
2022
accepted:
08
08
2022
pubmed:
31
8
2022
medline:
14
10
2022
entrez:
30
8
2022
Statut:
ppublish
Résumé
Bacteria require membrane fission for both cell division and endospore formation. In Bacillus subtilis, sporulation initiates with an asymmetric division that generates a large mother cell and a smaller forespore that contains only a quarter of its genome. As the mother cell membranes engulf the forespore, a DNA translocase pumps the rest of the chromosome into the small forespore compartment, inflating it due to increased turgor. When the engulfing membrane undergoes fission, the forespore is released into the mother cell cytoplasm. The B. subtilis protein FisB catalyzes membrane fission during sporulation, but the molecular basis is unclear. Here, we show that forespore inflation and FisB accumulation are both required for an efficient membrane fission. Forespore inflation leads to higher membrane tension in the engulfment membrane than in the mother cell membrane, causing the membrane to flow through the neck connecting the two membrane compartments. Thus, the mother cell supplies some of the membrane required for the growth of the membranes surrounding the forespore. The oligomerization of FisB at the membrane neck slows the equilibration of membrane tension by impeding the membrane flow. This leads to a further increase in the tension of the engulfment membrane, promoting its fission through lysis. Collectively, our data indicate that DNA translocation has a previously unappreciated second function in energizing the FisB-mediated membrane fission under energy-limited conditions.
Identifiants
pubmed: 36041438
pii: S0960-9822(22)01291-X
doi: 10.1016/j.cub.2022.08.014
pmc: PMC9730832
mid: NIHMS1830678
pii:
doi:
Substances chimiques
Bacterial Proteins
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4186-4200.e8Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM114513
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS113236
Pays : United States
Informations de copyright
Copyright © 2022 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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