Investigation of the effects of post-operative intraperitoneal, oral, and rectal phenytoin administration on colorectal anastomosis in rats.
Sıçanlarda ameliyat sonrası intraperitoneal, oral ve rektal fenitoin uygulamasının kolorektal anastomoz üzerine etkilerinin araştırılması.
Anastomosis, Surgical
/ adverse effects
Animals
Colon
/ surgery
Colorectal Neoplasms
/ pathology
Fibroblast Growth Factor 2
/ genetics
Phenytoin
/ metabolism
RNA, Messenger
/ metabolism
Rats
Rats, Wistar
Rectum
/ surgery
Transforming Growth Factor beta
/ metabolism
Vascular Endothelial Growth Factor A
/ genetics
Journal
Ulusal travma ve acil cerrahi dergisi = Turkish journal of trauma & emergency surgery : TJTES
ISSN: 1307-7945
Titre abrégé: Ulus Travma Acil Cerrahi Derg
Pays: Turkey
ID NLM: 101274231
Informations de publication
Date de publication:
Sep 2022
Sep 2022
Historique:
entrez:
31
8
2022
pubmed:
1
9
2022
medline:
9
9
2022
Statut:
ppublish
Résumé
Anastomotic leakage is the most feared complication after colonic anastomosis. The purpose of the study is to determine the effects of phenytoin applied by different application routes, on the healing process of colorectal anastomoses. Wistar Albino rats were divided into Intraperitoneal Phenytoin Group, Oral Phenytoin Group (OAP), Rectal Phenytoin Group (RAP), and control groups. The molecular effect of phenytoin on the expression of vascular endothelial growth factor (VEGF), transforming growth factor-beta (TGF-β), fibroblast growth factor 2 (FGF2), and p53 genes was evaluated at mRNA and protein level. The effects of phenytoin on anastomotic bursting pressure analysis measured as well as pathohistological examinations. There are statistically significant increase in anastomotic bursting pressure values between control and application groups. Inflammatory cell infiltration of all groups increased in the intestinal anastomosis region compared to control. Collagen scores were found to be significantly higher in the OAP and RAP groups compared to the control group. mRNA of TGF-ß and FGF2 expression increased in all routes of phenytoin applications. Three different administration routes show considerably increase on the bursting pressure. Regarding the results of the expression of FGF2, TGF-β, p53, and VEGF genes, there is a significant increase FGF2 and TGF-β at mRNA and protein level in most administration routes.
Sections du résumé
BACKGROUND
BACKGROUND
Anastomotic leakage is the most feared complication after colonic anastomosis. The purpose of the study is to determine the effects of phenytoin applied by different application routes, on the healing process of colorectal anastomoses.
METHODS
METHODS
Wistar Albino rats were divided into Intraperitoneal Phenytoin Group, Oral Phenytoin Group (OAP), Rectal Phenytoin Group (RAP), and control groups. The molecular effect of phenytoin on the expression of vascular endothelial growth factor (VEGF), transforming growth factor-beta (TGF-β), fibroblast growth factor 2 (FGF2), and p53 genes was evaluated at mRNA and protein level. The effects of phenytoin on anastomotic bursting pressure analysis measured as well as pathohistological examinations.
RESULTS
RESULTS
There are statistically significant increase in anastomotic bursting pressure values between control and application groups. Inflammatory cell infiltration of all groups increased in the intestinal anastomosis region compared to control. Collagen scores were found to be significantly higher in the OAP and RAP groups compared to the control group. mRNA of TGF-ß and FGF2 expression increased in all routes of phenytoin applications.
CONCLUSION
CONCLUSIONS
Three different administration routes show considerably increase on the bursting pressure. Regarding the results of the expression of FGF2, TGF-β, p53, and VEGF genes, there is a significant increase FGF2 and TGF-β at mRNA and protein level in most administration routes.
Identifiants
pubmed: 36043935
doi: 10.14744/tjtes.2022.03704
pmc: PMC10315947
doi:
Substances chimiques
RNA, Messenger
0
Transforming Growth Factor beta
0
Vascular Endothelial Growth Factor A
0
Fibroblast Growth Factor 2
103107-01-3
Phenytoin
6158TKW0C5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
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