Lactic acid from vaginal microbiota enhances cervicovaginal epithelial barrier integrity by promoting tight junction protein expression.
Epithelial cells
Female reproductive tract
HIV
Lactic acid
Lactobacilli
Metabolites
STIs
Tight junctions
Transcriptomics
Vaginal microbiome
Journal
Microbiome
ISSN: 2049-2618
Titre abrégé: Microbiome
Pays: England
ID NLM: 101615147
Informations de publication
Date de publication:
31 08 2022
31 08 2022
Historique:
received:
12
02
2022
accepted:
30
07
2022
entrez:
31
8
2022
pubmed:
1
9
2022
medline:
9
9
2022
Statut:
epublish
Résumé
Women with a cervicovaginal microbiota dominated by Lactobacillus spp. are at reduced risk of acquiring sexually transmitted infections including HIV, but the biological mechanisms involved remain poorly defined. Here, we performed metaproteomics on vaginal swab samples from young South African women (n = 113) and transcriptomics analysis of cervicovaginal epithelial cell cultures to examine the ability of lactic acid, a metabolite produced by cervicovaginal lactobacilli, to modulate genital epithelial barrier function. Compared to women with Lactobacillus-depleted microbiota, women dominated by vaginal lactobacilli exhibit higher abundance of bacterial lactate dehydrogenase, a key enzyme responsible for lactic acid production, which is independently associated with an increased abundance of epithelial barrier proteins. Physiological concentrations of lactic acid enhance epithelial cell culture barrier integrity and increase intercellular junctional molecule expression. These findings reveal a novel ability of vaginal lactic acid to enhance genital epithelial barrier integrity that may help prevent invasion by sexually transmitted pathogens. Video abstract.
Sections du résumé
BACKGROUND
Women with a cervicovaginal microbiota dominated by Lactobacillus spp. are at reduced risk of acquiring sexually transmitted infections including HIV, but the biological mechanisms involved remain poorly defined. Here, we performed metaproteomics on vaginal swab samples from young South African women (n = 113) and transcriptomics analysis of cervicovaginal epithelial cell cultures to examine the ability of lactic acid, a metabolite produced by cervicovaginal lactobacilli, to modulate genital epithelial barrier function.
RESULTS
Compared to women with Lactobacillus-depleted microbiota, women dominated by vaginal lactobacilli exhibit higher abundance of bacterial lactate dehydrogenase, a key enzyme responsible for lactic acid production, which is independently associated with an increased abundance of epithelial barrier proteins. Physiological concentrations of lactic acid enhance epithelial cell culture barrier integrity and increase intercellular junctional molecule expression.
CONCLUSIONS
These findings reveal a novel ability of vaginal lactic acid to enhance genital epithelial barrier integrity that may help prevent invasion by sexually transmitted pathogens. Video abstract.
Identifiants
pubmed: 36045402
doi: 10.1186/s40168-022-01337-5
pii: 10.1186/s40168-022-01337-5
pmc: PMC9429363
doi:
Substances chimiques
Tight Junction Proteins
0
Lactic Acid
33X04XA5AT
Types de publication
Journal Article
Video-Audio Media
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
141Subventions
Organisme : NIAID NIH HHS
ID : U01 AI070921
Pays : United States
Organisme : NIAID NIH HHS
ID : UH2 AI083264
Pays : United States
Organisme : NIAID NIH HHS
ID : U19 AI084044
Pays : United States
Informations de copyright
© 2022. The Author(s).
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