The TRESLIN-MTBP complex couples completion of DNA replication with S/G2 transition.
ATR
CHK1
DNA replication
MTBP
S/G2 transition
TRESLIN
cell cycle
checkpoint
origin firing
Journal
Molecular cell
ISSN: 1097-4164
Titre abrégé: Mol Cell
Pays: United States
ID NLM: 9802571
Informations de publication
Date de publication:
15 09 2022
15 09 2022
Historique:
received:
30
09
2021
revised:
16
05
2022
accepted:
04
08
2022
pubmed:
2
9
2022
medline:
21
9
2022
entrez:
1
9
2022
Statut:
ppublish
Résumé
It has been proposed that ATR kinase senses the completion of DNA replication to initiate the S/G2 transition. In contrast to this model, we show here that the TRESLIN-MTBP complex prevents a premature entry into G2 from early S-phase independently of ATR/CHK1 kinases. TRESLIN-MTBP acts transiently at pre-replication complexes (preRCs) to initiate origin firing and is released after the subsequent recruitment of CDC45. This dynamic behavior of TRESLIN-MTBP implements a monitoring system that checks the activation of replication forks and senses the rate of origin firing to prevent the entry into G2. This system detects the decline in the number of origins of replication that naturally occurs in very late S, which is the signature that cells use to determine the completion of DNA replication and permit the S/G2 transition. Our work introduces TRESLIN-MTBP as a key player in cell-cycle control independent of canonical checkpoints.
Identifiants
pubmed: 36049481
pii: S1097-2765(22)00759-6
doi: 10.1016/j.molcel.2022.08.006
pmc: PMC9506001
pii:
doi:
Substances chimiques
Carrier Proteins
0
Cell Cycle Proteins
0
DNA-Binding Proteins
0
Checkpoint Kinase 1
EC 2.7.11.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3350-3365.e7Informations de copyright
Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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