Molecular basis for polysaccharide recognition and modulated ATP hydrolysis by the O antigen ABC transporter.

ATP-Binding Cassette Transporters / metabolism Adenosine Triphosphate / metabolism Bacterial Proteins / metabolism Hydrolysis O Antigens / chemistry

Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
05 09 2022
Historique:
received: 27 05 2022
accepted: 08 08 2022
entrez: 6 9 2022
pubmed: 7 9 2022
medline: 9 9 2022
Statut: epublish

Résumé

O antigens are ubiquitous protective extensions of lipopolysaccharides in the extracellular leaflet of the Gram-negative outer membrane. Following biosynthesis in the cytosol, the lipid-linked polysaccharide is transported to the periplasm by the WzmWzt ABC transporter. Often, O antigen secretion requires the chemical modification of its elongating terminus, which the transporter recognizes via a carbohydrate-binding domain (CBD). Here, using components from A. aeolicus, we identify the O antigen structure with methylated mannose or rhamnose as its cap. Crystal and cryo electron microscopy structures reveal how WzmWzt recognizes this cap between its carbohydrate and nucleotide-binding domains in a nucleotide-free state. ATP binding induces drastic conformational changes of its CBD, terminating interactions with the O antigen. ATPase assays and site directed mutagenesis reveal reduced hydrolytic activity upon O antigen binding, likely to facilitate polymer loading into the ABC transporter. Our results elucidate critical steps in the recognition and translocation of polysaccharides by ABC transporters.

Identifiants

DOI: 10.1038/s41467-022-32597-2 PMID: 36064941 PMC: PMC9445017
pubmed: 36064941
doi: 10.1038/s41467-022-32597-2
pii: 10.1038/s41467-022-32597-2
pmc: PMC9445017
doi:

Substances chimiques

ATP-Binding Cassette Transporters 0
Bacterial Proteins 0
O Antigens 0
Adenosine Triphosphate 8L70Q75FXE

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

5226

Subventions

Organisme : Howard Hughes Medical Institute
Pays : United States
Organisme : NCRR NIH HHS
ID : S10 RR028976
Pays : United States
Organisme : NIH HHS
ID : S10 OD027000
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM144130
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM129666
Pays : United States

Informations de copyright

© 2022. The Author(s).

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Auteurs

Nicholas Spellmon (N)

Howard Hughes Medical Institute, University of Virginia School of Medicine, Charlottesville, VA, USA.
Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, VA, 22908, USA.
ORCID: 0000-0002-2045-1373

Artur Muszyński (A)

Complex Carbohydrate Research Center, University of Georgia, Athens, GA, 30602, USA.
ORCID: 0000-0003-3497-9977

Ireneusz Górniak (I)

Howard Hughes Medical Institute, University of Virginia School of Medicine, Charlottesville, VA, USA.
Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, VA, 22908, USA.
ORCID: 0000-0003-0357-6980

Jiri Vlach (J)

Complex Carbohydrate Research Center, University of Georgia, Athens, GA, 30602, USA.
ORCID: 0000-0002-0874-2055

David Hahn (D)

Complex Carbohydrate Research Center, University of Georgia, Athens, GA, 30602, USA.

Parastoo Azadi (P)

Complex Carbohydrate Research Center, University of Georgia, Athens, GA, 30602, USA.

Jochen Zimmer (J)

Howard Hughes Medical Institute, University of Virginia School of Medicine, Charlottesville, VA, USA. jz3x@virginia.edu.
Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, VA, 22908, USA. jz3x@virginia.edu.
ORCID: 0000-0002-8423-2882

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Transporteurs ABC › Molecular basis for polysaccharide recognition...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Ribonucléotides › Nucléotides adényliques › Adénosine triphosphate › Molecular basis for polysaccharide recognition...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Molecular basis for polysaccharide recognition...
questionsmedicales.fr › Phénomènes chimiques › Hydrolyse › Molecular basis for polysaccharide recognition...
questionsmedicales.fr › Facteurs biologiques › Toxines biologiques › Toxines bactériennes › Endotoxines › Lipopolysaccharides › Antigènes O › Molecular basis for polysaccharide recognition...