Mouse Models for Cytomegalovirus Infections in Newborns and Adults.

Animals Cytomegalovirus Infections Disease Models, Animal Mice Muromegalovirus Salivary Glands
MCMV cytomegalovirus infections disease models fibroblasts herpesviridae infections mice mouse cytomegalovirus salivary gland viral load viral plaque assay virology virus replication

Journal

Current protocols
ISSN: 2691-1299
Titre abrégé: Curr Protoc
Pays: United States
ID NLM: 101773894

Informations de publication

Date de publication:
Sep 2022
Historique:
entrez: 9 9 2022
pubmed: 10 9 2022
medline: 14 9 2022
Statut: ppublish

Résumé

This article describes procedures for infecting adult mice with murine cytomegalovirus (MCMV) and for infecting newborn mice to model congenital CMV infection. Methods are included for propagating MCMV in cell cultures and preparing a more virulent form of MCMV from the salivary glands of infected mice. A plaque assay is provided for determining MCMV titers of infected tissues or virus stocks. Also, methods are described for preparing the murine embryonic fibroblasts used for propagating MCMV, and for the plaque assay. © 2022 Wiley Periodicals LLC.

Identifiants

DOI: 10.1002/cpz1.537 PMID: 36083111
pubmed: 36083111
doi: 10.1002/cpz1.537
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e537

Subventions

Organisme : NIH HHS
ID : 1RO1AI089956-01A1
Pays : United States
Organisme : European Regional Development Fund
ID : KK.01.1.1.01.0006

Informations de copyright

© 2022 Wiley Periodicals LLC.

Auteurs

Ilija Brizić (I)

Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.

Berislav Lisnić (B)

Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.

Fran Krstanović (F)

Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.

Wolfram Brune (W)

Leibniz Institute for Virology, Hamburg, Germany.

Hartmut Hengel (H)

Institute of Virology, Medical Center-University of Freiburg, and Faculty of Medicine, University of Freiburg, Freiburg, Germany.

Stipan Jonjić (S)

Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Mouse Models for Cytomegalovirus Infections in...
questionsmedicales.fr › Infection › Maladies virales › Infections à virus à ADN › Infections à Herpesviridae › Infections à cytomégalovirus › Mouse Models for Cytomegalovirus Infections in...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles biologiques › Modèles animaux de maladie humaine › Mouse Models for Cytomegalovirus Infections in...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Mouse Models for Cytomegalovirus Infections in...
questionsmedicales.fr › Virus › Virus à ADN › Herpesviridae › Betaherpesvirinae › Muromegalovirus › Mouse Models for Cytomegalovirus Infections in...
questionsmedicales.fr › Système stomatognathique › Bouche › Glandes salivaires › Mouse Models for Cytomegalovirus Infections in...