Immortalized human myoblast cell lines for the delivery of therapeutic proteins using encapsulated cell technology.
allogeneic cell transplant
cell engineering
cell therapy
drug delivery
encapsulated cell technology
ex vivo gene therapy
macroencapsulation
myoblast
Journal
Molecular therapy. Methods & clinical development
ISSN: 2329-0501
Titre abrégé: Mol Ther Methods Clin Dev
Pays: United States
ID NLM: 101624857
Informations de publication
Date de publication:
08 Sep 2022
08 Sep 2022
Historique:
received:
20
04
2022
accepted:
31
07
2022
entrez:
12
9
2022
pubmed:
13
9
2022
medline:
13
9
2022
Statut:
epublish
Résumé
Despite many promising results obtained in previous preclinical studies, the clinical development of encapsulated cell technology (ECT) for the delivery of therapeutic proteins from macrocapsules is still limited, mainly due to the lack of an allogeneic cell line compatible with therapeutic application in humans. In our work, we generated an immortalized human myoblast cell line specifically tailored for macroencapsulation. In the present report, we characterized the immortalized myoblasts and described the engineering process required for the delivery of functional therapeutic proteins including a cytokine, monoclonal antibodies and a viral antigen. We observed that, when encapsulated, the novel myoblast cell line can be efficiently frozen, stored, and thawed, which limits the challenge imposed by the manufacture and supply of encapsulated cell-based therapeutic products. Our results suggest that this versatile allogeneic cell line represents the next step toward a broader development and therapeutic use of ECT.
Identifiants
pubmed: 36092361
doi: 10.1016/j.omtm.2022.07.017
pii: S2329-0501(22)00109-7
pmc: PMC9418741
doi:
Types de publication
Journal Article
Langues
eng
Pagination
441-458Commentaires et corrections
Type : ErratumIn
Informations de copyright
© 2022 The Authors.
Déclaration de conflit d'intérêts
The authors declare that they have no known competing financial interests or personal relationships that could have influenced the work reported in this paper. E.C., A.E., and J.G. are employees of MaxiVAX SA. A.L. is a former employee of MaxiVAX SA and a current scientific advisor. N.M is founder and minority shareholder of MaxiVAX SA. MaxiVAX SA is a Geneva-based biotech company involved in cell encapsulation technology.
Références
Skelet Muscle. 2011 Nov 01;1:34
pubmed: 22040608
Alzheimers Res Ther. 2016 Jul 07;8(1):30
pubmed: 27389402
Anal Biochem. 2010 Aug;403(1-2):94-101
pubmed: 20382104
Hum Gene Ther. 2004 Oct;15(10):968-75
pubmed: 15585112
Diabetes. 1994 Sep;43(9):1167-70
pubmed: 8070618
Exp Hematol. 1977 Nov;5(6):456-64
pubmed: 304012
Nat Biotechnol. 2001 Jan;19(1):35-9
pubmed: 11135549
Science. 2020 Mar 13;367(6483):1260-1263
pubmed: 32075877
J Biomol Screen. 1999;4(2):67-73
pubmed: 10838414
Int J Mol Sci. 2015 May 08;16(5):10578-600
pubmed: 26006227
Front Bioeng Biotechnol. 2019 Dec 03;7:380
pubmed: 31850335
Brain. 2016 May;139(Pt 5):1587-604
pubmed: 26956423
Mol Ther. 2003 Apr;7(4):506-14
pubmed: 12727114
Neuro Oncol. 2001 Jul;3(3):201-10
pubmed: 11465401
Blood Cancer J. 2021 Apr 6;11(4):69
pubmed: 33824268
Ann N Y Acad Sci. 2001 Nov;944:35-46
pubmed: 11797684
Cancer Gene Ther. 2011 Aug;18(8):553-62
pubmed: 21566667
Nat Neurosci. 2011 Dec 25;15(2):197-204
pubmed: 22197831
Stem Cells Transl Med. 2012 Oct;1(10):759-69
pubmed: 23197668
Mol Cell Biol. 1989 Nov;9(11):5022-33
pubmed: 2601707
Proc Natl Acad Sci U S A. 2011 Apr 12;108(15):6241-5
pubmed: 21444807
J Transl Med. 2016 Oct 12;14(1):288
pubmed: 27729044
Signal Transduct Target Ther. 2021 Feb 8;6(1):53
pubmed: 33558455
Transplant Proc. 1992 Oct;24(5):2291-2
pubmed: 1413062
Cancer Res. 2001 Sep 15;61(18):6830-7
pubmed: 11559558
Curr Protoc Neurosci. 2010 Oct;Chapter 4:Unit 4.21
pubmed: 20938923
Stem Cells Transl Med. 2015 Oct;4(10):1214-22
pubmed: 26304037
J Alzheimers Dis. 2012;29(1):187-200
pubmed: 22232015
Aging Cell. 2007 Aug;6(4):515-23
pubmed: 17559502
Transplantation. 1990 Feb;49(2):396-404
pubmed: 1968298
Transplantation. 1996 Jun 27;61(12):1678-84
pubmed: 8685943
Br Med Bull. 1997;53(4):777-88
pubmed: 9536527
Biomaterials. 2014 Jan;35(2):779-91
pubmed: 24103654
Trends Pharmacol Sci. 2015 Aug;36(8):537-46
pubmed: 26067102
Lancet. 2001 May 19;357(9268):1591-2
pubmed: 11377651
Development. 2012 Aug;139(16):2857-65
pubmed: 22764051
Gene Ther. 2012 Oct;19(10):1010-7
pubmed: 22113314
Biomaterials. 2002 Dec;23(24):4689-99
pubmed: 12361607
Nat Med. 1996 Jun;2(6):696-9
pubmed: 8640564
Proc Natl Acad Sci U S A. 1994 Jul 5;91(14):6496-500
pubmed: 8022811
Cancer Res. 2000 Jun 15;60(12):3239-46
pubmed: 10866317
Methods Mol Biol. 2011;737:183-209
pubmed: 21590398
Skelet Muscle. 2016 Dec 8;6(1):43
pubmed: 27931240
Diabetes Care. 2011 Nov;34(11):2406-9
pubmed: 21926290
Gene Ther. 1998 Aug;5(8):1014-22
pubmed: 10326023
Curr Res Pharmacol Drug Discov. 2020 May 06;1:19-29
pubmed: 34909639
Diabetes. 1999 Jul;48(7):1381-8
pubmed: 10389842
Gene Ther. 2001 Apr;8(7):523-33
pubmed: 11319619
Blood. 1996 Jun 15;87(12):5095-103
pubmed: 8652822
Nat Med. 1996 Aug;2(8):883-7
pubmed: 8705857
Science. 1991 Dec 20;254(5039):1782-4
pubmed: 1763328
Transplantation. 2000 Jul 15;70(1):78-85
pubmed: 10919579
Ann Oncol. 2007 Feb;18(2):226-32
pubmed: 17116643
Hum Gene Ther. 1996 Nov 10;7(17):2135-46
pubmed: 8934227
Exp Cell Res. 2021 Jun 1;403(1):112586
pubmed: 33839146
Methods Mol Biol. 2020;2100:473-491
pubmed: 31939144
Leukemia. 2018 Jul;32(7):1529-1541
pubmed: 29654266
Diabetes Care. 2009 Oct;32(10):1887-9
pubmed: 19549731
Transplant Proc. 2006 May;38(4):1156-7
pubmed: 16757293
Dev Biol Stand. 1998;93:177-201
pubmed: 9737396
Ophthalmology. 2019 Apr;126(4):540-549
pubmed: 30292541
Int J Cancer. 1979 Feb;23(2):143-7
pubmed: 367973
Ann N Y Acad Sci. 2001 Nov;944:226-39
pubmed: 11797672
Tissue Eng. 2000 Apr;6(2):151-63
pubmed: 10941210
Mol Cancer Res. 2003 Jul;1(9):643-53
pubmed: 12861050
J Neurosurg. 2012 Aug;117(2):340-7
pubmed: 22655593
Clin Cancer Res. 2013 Oct 1;19(19):5381-9
pubmed: 23788581
Kidney Int. 2002 Oct;62(4):1395-401
pubmed: 12234311
Mol Ther. 2010 Dec;18(12):2164-72
pubmed: 20664524
Am J Transplant. 2019 Jul;19(7):1930-1940
pubmed: 30748094
Biomaterials. 2014 Jan;35(2):792-802
pubmed: 24344356
J Neurol Sci. 2007 Apr 15;255(1-2):7-16
pubmed: 17341424