Zoonotic Enterocytozoon bieneusi genotypes in free-ranging and farmed wild ungulates in Spain.


Journal

Medical mycology
ISSN: 1460-2709
Titre abrégé: Med Mycol
Pays: England
ID NLM: 9815835

Informations de publication

Date de publication:
30 Sep 2022
Historique:
received: 29 06 2022
revised: 06 08 2022
accepted: 08 09 2022
pubmed: 13 9 2022
medline: 5 10 2022
entrez: 12 9 2022
Statut: ppublish

Résumé

Microsporidia comprises a diverse group of obligate, intracellular, and spore-forming parasites that infect a wide range of animals. Among them, Enterocytozoon bieneusi is the most frequently reported species in humans and other mammals and birds. Data on the epidemiology of E. bieneusi in wildlife are limited. Hence, E. bieneusi was investigated in eight wild ungulate species present in Spain (genera Ammotragus, Capra, Capreolus, Cervus, Dama, Ovis, Rupicapra, and Sus) by molecular methods. Faecal samples were collected from free-ranging (n = 1058) and farmed (n = 324) wild ungulates from five Spanish bioregions. The parasite was detected only in red deer (10.4%, 68/653) and wild boar (0.8%, 3/359). Enterocytozoon bieneusi infections were more common in farmed (19.4%, 63/324) than in wild (1.5%, 5/329) red deer. A total of 11 genotypes were identified in red deer, eight known (BEB6, BEB17, EbCar2, HLJD-V, MWC_d1, S5, Type IV, and Wildboar3) and three novel (DeerSpEb1, DeerSpEb2, and DeerSpEb3) genotypes. Mixed genotype infections were detected in 15.9% of farmed red deer. Two genotypes were identified in wild boar, a known (Wildboar3) and a novel (WildboarSpEb1) genotypes. All genotypes identified belonged to E. bieneusi zoonotic Groups 1 and 2. This study provides the most comprehensive epidemiological study of E. bieneusi in Spanish ungulates to date, representing the first evidence of the parasite in wild red deer populations worldwide. Spanish wild boars and red deer are reservoir of zoonotic genotypes of E. bieneusi and might play an underestimated role in the transmission of this microsporidian species to humans and other animals. The fungal-related intracellular parasite Enterocytozoon bieneusi is a worldwide public health and veterinary problem. Here we demonstrated that it was present in wild boar, and wild and farmed red deer in Spain, with genotypes potentially capable of infecting humans, posing a public health risk.

Autres résumés

Type: plain-language-summary (eng)
The fungal-related intracellular parasite Enterocytozoon bieneusi is a worldwide public health and veterinary problem. Here we demonstrated that it was present in wild boar, and wild and farmed red deer in Spain, with genotypes potentially capable of infecting humans, posing a public health risk.

Identifiants

pubmed: 36095135
pii: 6696380
doi: 10.1093/mmy/myac070
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Carlos III Health Institute
Organisme : Institute of Cosmos Sciences, University of Barcelona
ID : 13/051
Organisme : Ministry of Economy and Competitiveness
ID : CGL2012
Organisme : Spanish Ministry of Science and Innovation
ID : CGL2016-80543-P

Investigateurs

Carles Conejero (C)
Carlos González-Crespo (C)
Cristina Garrido (C)
Diana Gassó (D)
Diana Andrea Murillo (D)
Emmanuel Serrano (E)
Gregorio Mentaberre (G)
Irene Torres-Blas (I)
Josep Estruch (J)
Josep Pastor (J)
Jorge Ramón López-Olvera (J)
María Escobar-González (M)
Marta Valldeperes (M)
Montse Mesalles (M)
Omar López (O)
Raquel Álvarez (R)
Rafaela Cuenca (R)
Roser Velarde (R)
Santiago Lavín (S)

Informations de copyright

© The Author(s) 2022. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology.

Auteurs

Alejandro Dashti (A)

Parasitology Reference and Research Laboratory, Spanish National Centre for Microbiology, Health Institute Carlos III, Majadahonda, Madrid 28220, Spain.

Mónica Santín (M)

Environmental Microbial and Food Safety Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA.

Pamela C Köster (PC)

Parasitology Reference and Research Laboratory, Spanish National Centre for Microbiology, Health Institute Carlos III, Majadahonda, Madrid 28220, Spain.

Begoña Bailo (B)

Parasitology Reference and Research Laboratory, Spanish National Centre for Microbiology, Health Institute Carlos III, Majadahonda, Madrid 28220, Spain.

Sheila Ortega (S)

Parasitology Reference and Research Laboratory, Spanish National Centre for Microbiology, Health Institute Carlos III, Majadahonda, Madrid 28220, Spain.

Elena Imaña (E)

Parasitology Reference and Research Laboratory, Spanish National Centre for Microbiology, Health Institute Carlos III, Majadahonda, Madrid 28220, Spain.

Miguel Ángel Habela (MÁ)

Department of Animal Health, Veterinary Sciences Faculty, Extremadura University, Caceres 10003, Spain.

Antonio Rivero-Juarez (A)

Infectious Diseases Unit, Maimonides Institute for Biomedical Research (IMIBIC), University Hospital Reina Sofía, University of Córdoba, Córdoba 14004, Spain.

Joaquin Vicente (J)

SaBio Group, Institute for Game and Wildlife Research, IREC (UCLM-CSIC-JCCM), Ciudad Real 13005, Spain.

Maria C Arnal (MC)

Department of Animal Pathology, Veterinary Faculty, University of Zaragoza, Zaragoza 50013, Spain.

Daniel Fernández de Luco (DF)

Department of Animal Pathology, Veterinary Faculty, University of Zaragoza, Zaragoza 50013, Spain.

Patrocinio Morrondo (P)

INVESAGA Group, Department of Animal Pathology, Faculty of Veterinary, University of Santiago de Compostela, Lugo 27002, Spain.

José A Armenteros (JA)

Council of Development, Territory Planning and the Environment of the Principado de Asturias, Oviedo 33005, Spain.

Ana Balseiro (A)

Animal Health Department, Veterinary School, University of León, León 24007, Spain.
Animal Health Department, Mountain Livestock Institute (CSIC-University of León), León 24346, Spain.

Guillermo A Cardona (GA)

Livestock Laboratory, Regional Government of Álava, Vitoria-Gasteiz 01520, Spain.

Carlos Martínez-Carrasco (C)

Animal Health Department, University of Murcia, Regional Campus of International Excellence "Campus Mare Nostrum", Espinardo, Murcia 30100, Spain.

José Antonio Ortiz (JA)

Department of Veterinary and Research, Medianilla S.L. Benalup-Casas Viejas 11179, Spain.

Rafael Calero-Bernal (R)

SALUVET, Department of Animal Health, Faculty of Veterinary, Complutense University of Madrid, Madrid 28040, Spain.

David Carmena (D)

CIBER Infectious Diseases (CIBERINFEC), Health Institute Carlos III, Madrid 28029, Spain.
Parasitology Reference and Research Laboratory, Spanish National Centre for Microbiology, Health Institute Carlos III, Majadahonda, Madrid 28220, Spain.

David González-Barrio (D)

Parasitology Reference and Research Laboratory, Spanish National Centre for Microbiology, Health Institute Carlos III, Majadahonda, Madrid 28220, Spain.

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Classifications MeSH