Life at high temperature observed in vitro upon laser heating of gold nanoparticles.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
12 09 2022
12 09 2022
Historique:
received:
19
03
2021
accepted:
31
08
2022
entrez:
12
9
2022
pubmed:
13
9
2022
medline:
15
9
2022
Statut:
epublish
Résumé
Thermophiles are microorganisms that thrive at high temperature. Studying them can provide valuable information on how life has adapted to extreme conditions. However, high temperature conditions are difficult to achieve on conventional optical microscopes. Some home-made solutions have been proposed, all based on local resistive electric heating, but no simple commercial solution exists. In this article, we introduce the concept of microscale laser heating over the field of view of a microscope to achieve high temperature for the study of thermophiles, while maintaining the user environment in soft conditions. Microscale heating with moderate laser intensities is achieved using a substrate covered with gold nanoparticles, as biocompatible, efficient light absorbers. The influences of possible microscale fluid convection, cell confinement and centrifugal thermophoretic motion are discussed. The method is demonstrated with two species: (i) Geobacillus stearothermophilus, a motile thermophilic bacterium thriving around 65 °C, which we observed to germinate, grow and swim upon microscale heating and (ii) Sulfolobus shibatae, a hyperthermophilic archaeon living at the optimal temperature of 80 °C. This work opens the path toward simple and safe observation of thermophilic microorganisms using current and accessible microscopy tools.
Identifiants
pubmed: 36097020
doi: 10.1038/s41467-022-33074-6
pii: 10.1038/s41467-022-33074-6
pmc: PMC9468142
doi:
Substances chimiques
Gold
7440-57-5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5342Informations de copyright
© 2022. The Author(s).
Références
J Biomed Opt. 2015;20(12):126009
pubmed: 26720876
ACS Nano. 2012 Mar 27;6(3):2452-8
pubmed: 22305011
Nat Methods. 2019 Jul;16(7):611-614
pubmed: 31235884
Bioresour Technol. 2017 Dec;245(Pt B):1498-1506
pubmed: 28634129
ACS Nano. 2011 Jul 26;5(7):5457-62
pubmed: 21657203
Curr Opin Microbiol. 2005 Dec;8(6):649-55
pubmed: 16257257
Opt Express. 2009 Jul 20;17(15):13080-94
pubmed: 19654713
Front Microbiol. 2021 Aug 10;12:707124
pubmed: 34447359
ACS Nano. 2012 Aug 28;6(8):7227-33
pubmed: 22808995
J Bacteriol. 1999 Aug;181(16):5114-8
pubmed: 10438790
Appl Environ Microbiol. 2012 Mar;78(6):1670-4
pubmed: 22247169
Nanoscale. 2012 Aug 7;4(15):4301-26
pubmed: 22751683
FEMS Microbiol Rev. 2001 Apr;25(2):175-243
pubmed: 11250035
Microb Cell Fact. 2007 Mar 15;6:9
pubmed: 17359551
Small. 2018 Aug;14(32):e1801910
pubmed: 29995322
Bioresour Technol. 2019 May;280:478-488
pubmed: 30826176
World J Microbiol Biotechnol. 2016 Nov;32(11):179
pubmed: 27628339
Chem Rev. 2019 Jul 10;119(13):8087-8130
pubmed: 31125213
Nanoscale. 2015 Oct 14;7(38):15814-21
pubmed: 26355960
Curr Biol. 2020 Jul 20;30(14):2852-2859.e4
pubmed: 32502411
Sci Rep. 2019 Mar 15;9(1):4644
pubmed: 30874570
Chem Soc Rev. 2012 Mar 7;41(5):1665-76
pubmed: 21975514
Nat Methods. 2010 May;7(5):387-90
pubmed: 20383132
Extremophiles. 2012 Nov;16(6):863-70
pubmed: 23076519
Cell Mol Life Sci. 2001 Aug;58(9):1216-33
pubmed: 11577980
Environ Microbiol. 2021 Aug;23(8):4612-4630
pubmed: 34190379
Nanoscale. 2014 Aug 7;6(15):8984-9
pubmed: 24969322
Nat Methods. 2014 Sep;11(9):899-901
pubmed: 25166869
J Bacteriol. 1995 Oct;177(19):5473-9
pubmed: 7559332
Nat Commun. 2014;5:3173
pubmed: 24445431
Nat Mater. 2020 Sep;19(9):946-958
pubmed: 32807918
Food Microbiol. 2016 Aug;57:28-35
pubmed: 27052699
ACS Omega. 2016 Jul 06;1(1):2-8
pubmed: 31457112
Appl Environ Microbiol. 2014 Aug;80(15):4764-70
pubmed: 24858087
Front Microbiol. 2015 Nov 05;6:1209
pubmed: 26594201
Nat Methods. 2015 Sep;12(9):801-2
pubmed: 26317233
Gastrointest Endosc. 2020 Sep;92(3):524-534.e6
pubmed: 32360302
Science. 2009 Jul 10;325(5937):167-71
pubmed: 19589995
Adv Sci (Weinh). 2019 Jul 22;6(17):1900471
pubmed: 31508273
Proc Natl Acad Sci U S A. 2008 Aug 5;105(31):10949-54
pubmed: 18664583
Appl Opt. 2000 Nov 1;39(31):5715-20
pubmed: 18354568
RSC Adv. 2021 Mar 30;11(21):12500-12506
pubmed: 35423787
World J Microbiol Biotechnol. 2017 Jun;33(6):112
pubmed: 28470425
Science. 2003 Aug 15;301(5635):934
pubmed: 12920290
3 Biotech. 2016 Jun;6(1):81
pubmed: 28330151
Extremophiles. 1997 Feb;1(1):14-21
pubmed: 9680332
Nat Methods. 2015 Sep;12(9):803
pubmed: 26317235
Front Microbiol. 2016 Oct 14;7:1609
pubmed: 27790206
FEMS Microbiol Rev. 2018 Sep 1;42(5):543-578
pubmed: 29945179
Extremophiles. 2017 Jul;21(4):651-670
pubmed: 28508135
Nat Methods. 2015 Sep;12(9):802-3
pubmed: 26317234