Human-specific ARHGAP11B ensures human-like basal progenitor levels in hominid cerebral organoids.
ARHGAP11B
brain organoids
human-specific genes
neocortex development
neocortex evolution
Journal
EMBO reports
ISSN: 1469-3178
Titre abrégé: EMBO Rep
Pays: England
ID NLM: 100963049
Informations de publication
Date de publication:
07 11 2022
07 11 2022
Historique:
revised:
18
08
2022
received:
25
01
2022
accepted:
23
08
2022
pubmed:
14
9
2022
medline:
9
11
2022
entrez:
13
9
2022
Statut:
ppublish
Résumé
The human-specific gene ARHGAP11B has been implicated in human neocortex expansion. However, the extent of ARHGAP11B's contribution to this expansion during hominid evolution is unknown. Here we address this issue by genetic manipulation of ARHGAP11B levels and function in chimpanzee and human cerebral organoids. ARHGAP11B expression in chimpanzee cerebral organoids doubles basal progenitor levels, the class of cortical progenitors with a key role in neocortex expansion. Conversely, interference with ARHGAP11B's function in human cerebral organoids decreases basal progenitors down to the chimpanzee level. Moreover, ARHGAP11A or ARHGAP11B rescue experiments in ARHGAP11A plus ARHGAP11B double-knockout human forebrain organoids indicate that lack of ARHGAP11B, but not of ARHGAP11A, decreases the abundance of basal radial glia-the basal progenitor type thought to be of particular relevance for neocortex expansion. Taken together, our findings demonstrate that ARHGAP11B is necessary and sufficient to ensure the elevated basal progenitor levels that characterize the fetal human neocortex, suggesting that this human-specific gene was a major contributor to neocortex expansion during human evolution.
Identifiants
pubmed: 36098218
doi: 10.15252/embr.202254728
pmc: PMC9646322
mid: EMS155265
doi:
Substances chimiques
ARHGAP11B protein, human
0
GTPase-Activating Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e54728Subventions
Organisme : European Research Council
ID : 101039421
Pays : International
Informations de copyright
© 2022 The Authors. Published under the terms of the CC BY 4.0 license.
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