Hyperthermophilic endospores germinate and metabolize organic carbon in sediments heated to 80°C.
Journal
Environmental microbiology
ISSN: 1462-2920
Titre abrégé: Environ Microbiol
Pays: England
ID NLM: 100883692
Informations de publication
Date de publication:
11 2022
11 2022
Historique:
received:
13
10
2021
accepted:
10
08
2022
pubmed:
15
9
2022
medline:
19
11
2022
entrez:
14
9
2022
Statut:
ppublish
Résumé
Cold surface sediments host a seedbank of functionally diverse thermophilic bacteria. These thermophiles are present as endospores, which are widely dispersed in aquatic environments. Here, we investigated the functional potential of endospore populations in cold surface sediments heated to 80°C. Microbial production of acetate was observed at 80°C and could be enhanced by supplying additional organic carbon substrates. Comparison of 16S rRNA gene amplicon libraries from 80°C enrichments to sediments heated to lower temperatures (50-70°C) showed that temperature selects for distinct populations of endospore-forming bacteria. Whereas sulfate-reducing thermophiles were enriched in 50-70°C incubations, 80°C exceeds their thermal tolerance and selects for hyperthermophilic organotrophic bacteria that are similarly detected in amplicon libraries from sediments heated to 90°C. Genome-resolved metagenomics revealed novel carbon cycling members of Symbiobacteriales, Thermosediminibacteraceae, Thermanaeromonas and Calditerricola with the genomic potential for the degradation of carbohydrates, sugars, amino acids and nucleotides. Endospores of thermophilic bacteria are deposited on seabed sediments worldwide where they remain dormant as they are buried in the accumulating sediments. Our results suggest that endospore populations could be activated by temperature increases encountered during burial and show the potential for organotrophic metabolic activity contributing to acetate generation in deep hot sediments.
Identifiants
pubmed: 36100999
doi: 10.1111/1462-2920.16167
pmc: PMC9826295
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Carbon
7440-44-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5534-5545Informations de copyright
© 2022 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.
Références
PeerJ. 2019 Jul 26;7:e7359
pubmed: 31388474
Nucleic Acids Res. 2007 Jan;35(Database issue):D260-4
pubmed: 17151080
FEMS Microbiol Ecol. 2016 Jan;92(1):
pubmed: 26613749
Microbiology (Reading). 2010 Mar;156(Pt 3):967-974
pubmed: 20007648
Nat Commun. 2022 Jan 25;13(1):312
pubmed: 35078973
PLoS One. 2017 Oct 26;12(10):e0185056
pubmed: 29073143
Proc Natl Acad Sci U S A. 2012 Oct 2;109(40):16213-6
pubmed: 22927371
Science. 2009 Sep 18;325(5947):1541-4
pubmed: 19762643
Nat Microbiol. 2018 Jul;3(7):836-843
pubmed: 29807988
Microbiome. 2022 Feb 16;10(1):33
pubmed: 35172890
Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):2940-2945
pubmed: 28242677
Bioinformatics. 2015 May 15;31(10):1674-6
pubmed: 25609793
Int J Syst Evol Microbiol. 2011 Mar;61(Pt 3):631-636
pubmed: 20400666
Front Microbiol. 2017 Feb 06;8:131
pubmed: 28220111
Nat Rev Microbiol. 2008 Aug;6(8):613-24
pubmed: 18628769
Nat Commun. 2018 Nov 27;9(1):4999
pubmed: 30479325
ISME J. 2021 Dec;15(12):3480-3497
pubmed: 34112968
FEMS Microbiol Rev. 2015 Sep;39(5):688-728
pubmed: 25994609
Nat Methods. 2014 Nov;11(11):1144-6
pubmed: 25218180
ISME J. 2017 Dec;11(12):2864-2868
pubmed: 28742071
Nat Methods. 2013 Oct;10(10):996-8
pubmed: 23955772
Science. 2015 Jul 24;349(6246):420-4
pubmed: 26206933
ISME J. 2012 Jun;6(6):1089-93
pubmed: 22071345
Science. 2020 Dec 4;370(6521):1230-1234
pubmed: 33273103
Biodegradation. 1990;1(1):1-7
pubmed: 1368138
Sci Rep. 2017 Mar 22;7:44864
pubmed: 28327661
Nucleic Acids Res. 2013 Jan;41(Database issue):D590-6
pubmed: 23193283
Nat Microbiol. 2021 Jul;6(7):885-898
pubmed: 34127845
ISME J. 2013 Jan;7(1):72-84
pubmed: 22832348
mSystems. 2020 Oct 27;5(5):
pubmed: 33109753
Annu Rev Microbiol. 2017 Sep 8;71:459-477
pubmed: 28697670
Nucleic Acids Res. 2014 Jan;42(Database issue):D222-30
pubmed: 24288371
mBio. 2018 Jun 19;9(3):
pubmed: 29921664
Genome Res. 2017 May;27(5):824-834
pubmed: 28298430
J Appl Microbiol. 2006 Sep;101(3):514-25
pubmed: 16907802
Nat Biotechnol. 2020 Sep;38(9):1079-1086
pubmed: 32341564
Bioinformatics. 2019 Oct 15;35(20):4162-4164
pubmed: 30865266
ACS Synth Biol. 2019 Jun 21;8(6):1411-1420
pubmed: 31117361
PLoS Comput Biol. 2011 Oct;7(10):e1002195
pubmed: 22039361
Nature. 2001 Jun 28;411(6841):1034-7
pubmed: 11429600
Appl Environ Microbiol. 2016 Jun 30;82(14):4232-43
pubmed: 27208118
Microbiology (Reading). 2005 Mar;151(Pt 3):737-747
pubmed: 15758220
Extremophiles. 2005 Oct;9(5):375-83
pubmed: 15965715
PLoS One. 2013 Apr 24;8(4):e61928
pubmed: 23637931
Mol Ecol Resour. 2015 Nov;15(6):1403-14
pubmed: 25732605
Curr Opin Microbiol. 2008 Apr;11(2):87-93
pubmed: 18359269
Mol Biol Evol. 2015 Jan;32(1):268-74
pubmed: 25371430
ISME J. 2014 Jun;8(6):1153-65
pubmed: 24351936
Front Microbiol. 2017 Feb 06;8:137
pubmed: 28220112
FEMS Microbiol Ecol. 2015 Aug;91(8):fiv084
pubmed: 26207045
Bioinformatics. 2009 Aug 1;25(15):1972-3
pubmed: 19505945
Proc Natl Acad Sci U S A. 2008 Aug 5;105(31):10949-54
pubmed: 18664583
Metab Eng. 2018 Jul;48:208-217
pubmed: 29792931
Int J Syst Evol Microbiol. 2009 May;59(Pt 5):1100-7
pubmed: 19406800
FEMS Microbiol Ecol. 2002 Oct 1;42(1):59-70
pubmed: 19709266
Science. 2003 Aug 15;301(5635):934
pubmed: 12920290
Environ Microbiol. 2012 Nov;14(11):2870-90
pubmed: 22882546
Environ Microbiol. 2022 Nov;24(11):5534-5545
pubmed: 36100999
Nature. 2012 Mar 18;484(7392):101-4
pubmed: 22425999
BMC Bioinformatics. 2010 Mar 08;11:119
pubmed: 20211023
J Bacteriol. 1969 Apr;98(1):62-8
pubmed: 4977690
Nat Methods. 2010 May;7(5):335-6
pubmed: 20383131
Nat Methods. 2017 Jun;14(6):587-589
pubmed: 28481363
Nucleic Acids Res. 2016 Jan 4;44(D1):D343-50
pubmed: 26527717
Bioinformatics. 2019 Nov 15;:
pubmed: 31730192
Sci Adv. 2019 Feb 20;5(2):eaav1024
pubmed: 30801015
FEMS Microbiol Rev. 2020 Mar 1;44(2):219-231
pubmed: 32065239
Microbiol Mol Biol Rev. 2006 Dec;70(4):939-1031
pubmed: 17158705
Environ Microbiol. 2020 Jan;22(1):456-465
pubmed: 31742859
Int J Syst Evol Microbiol. 2002 Sep;52(Pt 5):1675-1680
pubmed: 12361273
J Mol Biol. 1990 Oct 5;215(3):403-10
pubmed: 2231712
Nature. 2003 Nov 20;426(6964):344-52
pubmed: 14628064
Nucleic Acids Res. 2004 Mar 19;32(5):1792-7
pubmed: 15034147
Bioinformatics. 2020 Apr 1;36(7):2251-2252
pubmed: 31742321
J Bacteriol. 2018 Jul 25;200(16):
pubmed: 29760211
FEMS Microbiol Ecol. 2018 Dec 1;94(12):
pubmed: 30247566
Mol Biol Evol. 2013 May;30(5):1188-95
pubmed: 23418397
Nucleic Acids Res. 2018 Jul 2;46(W1):W95-W101
pubmed: 29771380