Bioactive Fibronectin-III
DNA nanotechnology
biomaterials
bionanotechnology
coiled-coils
fibronectin
self-assembly
supramolecular chemistry
Journal
ACS applied bio materials
ISSN: 2576-6422
Titre abrégé: ACS Appl Bio Mater
Pays: United States
ID NLM: 101729147
Informations de publication
Date de publication:
15 Sep 2022
15 Sep 2022
Historique:
pmc-release:
15
03
2024
entrez:
15
9
2022
pubmed:
16
9
2022
medline:
16
9
2022
Statut:
aheadofprint
Résumé
The integration of proteins with DNA nanotechnology would enable materials with diverse applications in biology, medicine, and engineering. Here, we describe a method for the incorporation of bioactive fibronectin domain proteins with DNA nanostructures using two orthogonal coiled-coil peptides. One peptide from each coiled-coil pair is attached to a DNA origami cuboid in a multivalent fashion by attaching the peptides to DNA handles. These structures can then be assembled into one-dimensional arrays through the addition of a fibronectin domain linker genetically fused with the complementary peptides to those on the origami. We validate array formation using two different self-assembly protocols and characterize the fibers by atomic force and electron microscopy. Finally, we demonstrate that surfaces coated with the protein-DNA nanofibers can serve as biomaterial substrates for fibroblast adhesion and spreading with the nanofibers showing enhanced bioactivity compared to that of the monomeric protein.
Identifiants
pubmed: 36108278
doi: 10.1021/acsabm.2c00303
pmc: PMC10014493
mid: NIHMS1839819
doi:
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : DP2 GM132931
Pays : United States
Références
Elife. 2021 Jun 03;10:
pubmed: 34080973
ACS Appl Mater Interfaces. 2021 May 12;13(18):21018-21029
pubmed: 33938725
Annu Rev Biochem. 1999;68:729-77
pubmed: 10872465
FEBS Lett. 1994 Mar 7;340(3):197-201
pubmed: 8131845
Chem Rev. 2019 May 22;119(10):6384-6458
pubmed: 30714731
Bioinformatics. 2014 Nov 1;30(21):3029-35
pubmed: 25064570
J Am Chem Soc. 2020 Jan 22;142(3):1406-1416
pubmed: 31820959
Annu Rev Cell Dev Biol. 2010;26:397-419
pubmed: 20690820
Nat Commun. 2017 Jul 10;8:15982
pubmed: 28691701
ACS Nano. 2019 Jan 22;13(1):728-736
pubmed: 30588806
Faraday Discuss. 2019 Oct 30;219(0):203-219
pubmed: 31314021
Org Biomol Chem. 2019 Feb 13;17(7):1668-1682
pubmed: 30483688
Chem Soc Rev. 2010 Sep;39(9):3541-75
pubmed: 20676430
Sci Rep. 2015 Sep 15;5:14063
pubmed: 26370878
Biochemistry. 2000 Aug 1;39(30):8728-34
pubmed: 10913284
Chem Rev. 2017 Oct 25;117(20):12584-12640
pubmed: 28605177
FASEB J. 2010 Dec;24(12):4711-21
pubmed: 20671107
Chem Biol. 2003 Dec;10(12):1151-9
pubmed: 14700623
Nano Lett. 2015 Jan 14;15(1):603-9
pubmed: 25546084
Angew Chem Int Ed Engl. 2010 Nov 22;49(48):9278-81
pubmed: 20957712
J Am Chem Soc. 2010 Oct 27;132(42):14727-9
pubmed: 20925350
Science. 2013 May 3;340(6132):595-9
pubmed: 23579496
Nano Lett. 2016 Dec 14;16(12):7870-7874
pubmed: 27802042
Chem Rev. 2006 Sep;106(9):3712-61
pubmed: 16967918
Cell. 2006 Aug 25;126(4):677-89
pubmed: 16923388
Nature. 2006 Mar 16;440(7082):297-302
pubmed: 16541064
Sci Transl Med. 2011 Sep 14;3(100):100ra89
pubmed: 21918106
Nat Mater. 2009 Jul;8(7):596-600
pubmed: 19543314
Sci Rep. 2017 Jan 11;7:39805
pubmed: 28074920
J Pept Sci. 2011 Feb;17(2):100-6
pubmed: 21234981
Chem Soc Rev. 2011 Dec;40(12):5910-21
pubmed: 21975573
J Mol Biol. 1994 Mar 4;236(4):1079-92
pubmed: 8120888
ACS Nano. 2021 Feb 23;15(2):3441-3452
pubmed: 33556239
Nat Chem Biol. 2013 Jun;9(6):362-6
pubmed: 23624438
Proc Natl Acad Sci U S A. 2021 Jan 26;118(4):
pubmed: 33468643
Nano Lett. 2018 Aug 8;18(8):4803-4811
pubmed: 29911385