Widespread association of the Argonaute protein AGO2 with meiotic chromatin suggests a distinct nuclear function in mammalian male reproduction.
Journal
Genome research
ISSN: 1549-5469
Titre abrégé: Genome Res
Pays: United States
ID NLM: 9518021
Informations de publication
Date de publication:
15 Sep 2022
15 Sep 2022
Historique:
received:
11
01
2022
accepted:
21
07
2022
entrez:
15
9
2022
pubmed:
16
9
2022
medline:
16
9
2022
Statut:
aheadofprint
Résumé
Argonaute 2 (AGO2) is a ubiquitously expressed protein critical for regulation of mRNA translation and vital to animal development. AGO2 protein is found in both cytoplasmic and nuclear compartments, and although its cytoplasmic role is well studied, the biological relevance of nuclear AGO2 is unclear. Here, we address this problem in vivo using spermatogenic cells as a model. We find that AGO2 transiently binds both chromatin and nucleus-specific mRNA transcripts of hundreds of genes required for sperm production during male meiosis in mice, and that germline conditional knockout (cKO) of
Identifiants
pubmed: 36109149
pii: gr.276578.122
doi: 10.1101/gr.276578.122
pmc: PMC9528986
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIH HHS
ID : S10 OD018034
Pays : United States
Organisme : NIH HHS
ID : S10 OD019967
Pays : United States
Organisme : NIDDK NIH HHS
ID : U54 DK106857
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK124788
Pays : United States
Organisme : NICHD NIH HHS
ID : R01 HD098128
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR001863
Pays : United States
Organisme : NICHD NIH HHS
ID : R01 HD096745
Pays : United States
Organisme : NIDDK NIH HHS
ID : U24 DK126127
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK102792
Pays : United States
Informations de copyright
© 2022 Griffin et al.; Published by Cold Spring Harbor Laboratory Press.
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