Quantification of Plasma S-adenosylmethionine and S-adenosylhomocysteine Using Liquid Chromatography-Electrospray-Tandem Mass Spectrometry.

Chromatography, Liquid / methods Methanol S-Adenosylhomocysteine S-Adenosylmethionine Tandem Mass Spectrometry / methods
Mass spectrometry Methylation S-adenosylhomocysteine S-adenosylmethionine

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2022
Historique:
entrez: 20 9 2022
pubmed: 21 9 2022
medline: 24 9 2022
Statut: ppublish

Résumé

We describe a simple stable isotope dilution method for accurate determination of S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) in plasma as a clinical diagnostic test. Determination of SAM/SAH in plasma (20 μL) was performed by high-performance liquid chromatography coupled with electrospray positive ionization tandem mass spectrometry (HPLC-ESI-MS/MS). Calibrators (SAM and SAH) and internal standards (

Identifiants

DOI: 10.1007/978-1-0716-2565-1_4 PMID: 36127576
pubmed: 36127576
doi: 10.1007/978-1-0716-2565-1_4
doi:

Substances chimiques

S-Adenosylmethionine 7LP2MPO46S
S-Adenosylhomocysteine 979-92-0
Methanol Y4S76JWI15

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

35-43

Informations de copyright

© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Références

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doi: 10.1093/jn/135.11.2703 pubmed: 16251634
Hoffman DR, Cornatzer WE, Duerre JA (1979) Relationship between tissue levels of S-adenosylmethionine, S-adenosylhomocysteine and transmethylation reactions. Can J Biochem 57:56–65
doi: 10.1139/o79-007 pubmed: 427630
Yi P, Melnyk S, Pogribna M, Pogribny IP, Hine RJ, James SJ (2000) Parallel increases in plasma S-adenosylhomocysteine and lymphocyte DNA hypomethylation. J Biol Chem 275(38):239318–239323
doi: 10.1074/jbc.M002725200
Cantoni GL (1985) The role of S-adenosylhomocysteine in the biological utilization of S-adenosylmethionine. Prog Clin Biol Res 198:47–65
pubmed: 4070312
Mudd SH (2011) Hypermethioninemias of genetic and non-genetic origin: a review. Am J Med Genet C Semin Med Genet 157C(1):3–32
doi: 10.1002/ajmg.c.30293 pubmed: 21308989
Baric I, Staufner C, Augoustides-Savvopoulou P, Chien YH, Dobbelaere D, Grunert SC, Opladen T, Ramadza DP, Rakic B, Wedell A, Blom HJ (2017) Consensus recommendations for the diagnosis, treatment and follow-up of inherited methylation disorders. J Inherit Metab Dis 40:5–20
doi: 10.1007/s10545-016-9972-7 pubmed: 27671891
Bottiglieri T (2002) S-Adenosyl-L-methionine (SAMe): from the bench to the bedside–molecular basis of a pleiotropic molecule. Am J Clin Nutr 76(5):1151S–1157S
doi: 10.1093/ajcn/76.5.1151S pubmed: 12418493
Noureddin M, Sander-Struckmeier S, Mato JM (2020) Early treatment efficacy of S-adenosylmethionine in patients with intrahepatic cholestasis: a systematic review. World J Hepatol 12(2):46–63
doi: 10.4254/wjh.v12.i2.46 pubmed: 32184941 pmcid: 7061266
Cuomo A, Beccarini Crescenzi B, Bolognesi S, Goracci A, Koukouna D, Rossi R, Fagiolini A (2020) S-Adenosylmethionine (SAMe) in major depressive disorder (MDD): a clinician-oriented systematic review. Ann Gen Psychiatry 19:50
doi: 10.1186/s12991-020-00298-z pubmed: 32939220 pmcid: 7487540

Auteurs

Erland Arning (E)

Institute of Metabolic Disease, Baylor Scott & White Research Institute, Dallas, TX, USA. Erland.Arning@BSWHealth.org.

Brandi Wasek (B)

Institute of Metabolic Disease, Baylor Scott & White Research Institute, Dallas, TX, USA.

Teodoro Bottiglieri (T)

Institute of Metabolic Disease, Baylor Scott & White Research Institute, Dallas, TX, USA.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Quantification of Plasma S-adenosylmethionine...
questionsmedicales.fr › Composés chimiques organiques › Alcools › Méthanol › Quantification of Plasma S-adenosylmethionine...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléosides › Ribonucléosides › Adénosine › S-(5'-Désoxy-adénosyl)homocystéine › Quantification of Plasma S-adenosylmethionine...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléosides › Ribonucléosides › Adénosine › Adémétionine › Quantification of Plasma S-adenosylmethionine...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse en tandem › Quantification of Plasma S-adenosylmethionine...