Unusual "Asian-origin" 2c to 2b point mutant canine parvovirus (Parvoviridae) and canine astrovirus (Astroviridae) co-infection detected in vaccinated dogs with an outbreak of severe haemorrhagic gastroenteritis with high mortality rate in Hungary.
Canine Parvovirus 2
Canine astrovirus
Co-infection
Cultivation
Gastroenteritis
NGS
Journal
Veterinary research communications
ISSN: 1573-7446
Titre abrégé: Vet Res Commun
Pays: Switzerland
ID NLM: 8100520
Informations de publication
Date de publication:
Dec 2022
Dec 2022
Historique:
received:
20
06
2022
accepted:
06
09
2022
pubmed:
22
9
2022
medline:
26
11
2022
entrez:
21
9
2022
Statut:
ppublish
Résumé
In this study, the aetiological background of an outbreak of severe haemorrhagic gastroenteritis (HGE) in a colony of purebred Jack Russell Terriers vaccinated against CPV-2 in Hungary was investigated. Canine parvovirus 2 (CPV-2, Parvoviridae) and canine astrovirus (CaAstV, Astroviridae) co-infection was identified by viral metagenomics and next-generation sequencing (VM-NGS) methods from a rectal swab of an affected 7-week-old puppy. The complete coding sequence of CPV-2 strain FR1/CPV2-2021-HUN (ON733252) and the complete genome of CaAstV strain FR1/CaAstV-2021-HUN (ON733251) were determined by VM-NGS and PCR methods. Results of sequence and phylogenetic analyses showed that CPV-2 strain FR1/CPV2-2021-HUN was different from the applied vaccine strains and previously identified strains from Hungary but showed high sequence identity (> 99.8%) and close phylogenetic relationship to recently described "Asian-origin" CPV-2c strains from Italy. But, based on the single amino acid difference on position 426 of VP2 (Glu/Asp) between the study strain and the closest relatives, FR1/CPV2-2021-HUN belonged to the 2b antigenic type rather than 2c. The CaAstV strain FR1/CaAstV-2021-HUN showed close relationship with a CaAstV strain identified previously from a diarrhoeic dog in Hungary. Both viruses were continuously detectable by PCR in additional enteric samples, and the CPV-2 could also be detected in several (n = 32) tissue samples from 9 affected deceased puppies. Further comparative studies are necessary to confirm the role of the point mutation causing the change in the antigenic type of this "Asian-origin" CPV-2 and/or the role of CaAstV co-infection in the development and/or severity of (haemorrhagic) gastroenteritis among dogs vaccinated against CPV-2.
Identifiants
pubmed: 36129562
doi: 10.1007/s11259-022-09997-2
pii: 10.1007/s11259-022-09997-2
pmc: PMC9490711
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1355-1361Subventions
Organisme : Hungarian Scientific Research Fund
ID : FK134311
Informations de copyright
© 2022. The Author(s).
Références
Balboni A, Niculae M, Di Vito S, Urbani L, Terrusi A, Muresan C, Battilani M (2021) The detection of canine parvovirus type 2c of Asian origin in dogs in Romania evidenced its progressive worldwide diffusion. BMC Vet Res 17(1):206. https://doi.org/10.1186/s12917-021-02918-6
doi: 10.1186/s12917-021-02918-6
pubmed: 34090429
pmcid: 8180150
Bhatta TR, Chamings A, Vibin J, Alexandersen S (2019) Detection and characterisation of canine astrovirus, canine parvovirus and canine papillomavirus in puppies using next generation sequencing. Sci Rep 14 9(1):4602. https://doi.org/10.1038/s41598-019-41045-z
doi: 10.1038/s41598-019-41045-z
Caddy SL, Goodfellow I (2015) Complete genome sequence of canine astrovirus with molecular and epidemiological characterisation of UK strains. Vet Microbiol 15 177(1–2):206–13. https://doi.org/10.1016/j.vetmic.2015.03.011
doi: 10.1016/j.vetmic.2015.03.011
Chen B, Zhang X, Zhu J, Liao L, Bao E (2021) Molecular Epidemiological Survey of Canine Parvovirus Circulating in China from 2014 to 2019. Pathogens 11 10(5):588. https://doi.org/10.3390/pathogens10050588
doi: 10.3390/pathogens10050588
Cotmore SF, Agbandje-McKenna M, Canuti M, Chiorini JA, Eis-Hubinger AM, Hughes J et al (2019) ICTV report consortium ICTV virus taxonomy profile: Parvoviridae. J Gen Virol 100(3):367–8. https://doi.org/10.1099/jgv.0.001212
doi: 10.1099/jgv.0.001212
pubmed: 30672729
pmcid: 6537627
Cságola A, Varga S, Lőrincz M, Tuboly T (2014) Analysis of the full-length VP2 protein of canine parvoviruses circulating in Hungary. Arch Virol 159(9):2441–2444. https://doi.org/10.1007/s00705-014-2068-5
doi: 10.1007/s00705-014-2068-5
pubmed: 24696272
Decaro N, Buonavoglia C (2012) Canine parvovirus - a review of epidemiological and diagnostic aspects, with emphasis on type 2c. Vet Microbiol 24 155(1):1–12. https://doi.org/10.1016/j.vetmic.2011.09.007
doi: 10.1016/j.vetmic.2011.09.007
Decaro N, Desario C, Elia G, Martella V, Mari V, Lavazza A et al (2008) Evidence for immunisation failure in vaccinated adult dogs infected with canine parvovirus type 2c. New Microbiol 31(1):125–130
pubmed: 18437851
Demeter Z, Palade EA, Soós T, Farsang A, Jakab C, Rusvai M (2010) Misleading results of the MboII-based identification of type 2a canine parvovirus strains from Hungary reacting as type 2c strains. Virus Genes 41(1):37–42. https://doi.org/10.1007/s11262-010-0478-3
doi: 10.1007/s11262-010-0478-3
pubmed: 20390334
Mihalov-Kovács E, Martella V, Lanave G, Bodnar L, Fehér E, Marton S et al (2017) Genome analysis of canine astroviruses reveals genetic heterogeneity and suggests possible inter-species transmission. Virus Res 15(232):162–170. https://doi.org/10.1016/j.virusres.2016.12.005
doi: 10.1016/j.virusres.2016.12.005
Mira F, Purpari G, Di Bella S, Colaianni ML, Schirò G, Chiaramonte G et al (2019) Spreading of canine parvovirus type 2c mutants of Asian origin in southern Italy. Transbound Emerg Dis 66(6):2297–2304. https://doi.org/10.1111/tbed.13283
doi: 10.1111/tbed.13283
pubmed: 31254456
pmcid: 7159351
Miranda C, Thompson G (2016) Canine parvovirus: the worldwide occurrence of antigenic variants. J Gen Virol 97(9):2043–2057. https://doi.org/10.1099/jgv.0.000540
doi: 10.1099/jgv.0.000540
pubmed: 27389721
Novosel D, Tuboly T, Balka G, Szeredi L, Lojkic I, Jungic A et al (2019) Evidence of CPV2c introgression into Croatia and novel insights into phylogeny and cell tropism. Sci Rep 15 9(1):16909. https://doi.org/10.1038/s41598-019-53422-9
doi: 10.1038/s41598-019-53422-9
Qi S, Zhao J, Guo D, Sun D (2020) A mini-review on the epidemiology of canine parvovirus in China. Front Vet Sci 20(7):5. https://doi.org/10.3389/fvets.2020.00005
doi: 10.3389/fvets.2020.00005
Reed AP, Jones EV, Miller TJ (1988) Nucleotide sequence and genome organization of canine parvovirus. J Virol 62(1):266–276. https://doi.org/10.1128/JVI.62.1.266-276.1988
doi: 10.1128/JVI.62.1.266-276.1988
pubmed: 2824850
pmcid: 250527
Stamelou E, Giantsis IA, Papageorgiou KV, Petridou E, Davidson I, Polizopοulou ZS et al (2022) First report of canine Astrovirus and Sapovirus in Greece, hosting both asymptomatic and gastroenteritis symptomatic dogs. Virol J 19(1):1–9. https://doi.org/10.1186/s12985-022-01787-1
doi: 10.1186/s12985-022-01787-1
Temeeyasen G, Sharafeldin TA, Lin CM (2020) Hause BM (2022) Spillover of Canine Parvovirus Type 2 to Pigs, South Dakota, USA. Emerg Infect Dis 28(2):471–473. https://doi.org/10.3201/eid2802.211681
doi: 10.3201/eid2802.211681
Tsao J, Chapman MS, Agbandje M, Keller W, Smith K, Wu H et al (1991) The three-dimensional structure of canine parvovirus and its functional implications. Science 251(5000):1456–1464. https://doi.org/10.1126/science.2006420
doi: 10.1126/science.2006420
pubmed: 2006420
Tuteja D, Banu K, Mondal B (2022) Canine parvovirology - A brief updated review on structural biology, occurrence, pathogenesis, clinical diagnosis, treatment and prevention. Comp Immunol Microbiol Infect Dis 11(82):101765. https://doi.org/10.1016/j.cimid.2022.101765
doi: 10.1016/j.cimid.2022.101765
Voorhees IE, Lee H, Allison AB, Lopez-Astacio R, Goodman LB, Oyesola OO et al (2019) Limited intrahost diversity and background evolution accompany 40 years of canine parvovirus host adaptation and spread. J Virol 12 94(1):e01162-19. https://doi.org/10.1128/JVI.01162-19
doi: 10.1128/JVI.01162-19