EPR Spectroscopy Provides New Insights into Complex Biological Reaction Mechanisms.


Journal

The journal of physical chemistry. B
ISSN: 1520-5207
Titre abrégé: J Phys Chem B
Pays: United States
ID NLM: 101157530

Informations de publication

Date de publication:
06 10 2022
Historique:
pubmed: 23 9 2022
medline: 12 10 2022
entrez: 22 9 2022
Statut: ppublish

Résumé

In the last 20 years, the use of electron paramagnetic resonance (EPR) has made a pronounced and lasting impact in the field of structural biology. The advantage of EPR spectroscopy over other structural techniques is its ability to target even minor conformational changes in any biomolecule or macromolecular complex, independent of its size or complexity, or whether it is in solution or in the cell during a biological or chemical reaction. Here, we focus on the use of EPR spectroscopy to study transmembrane transport and transcription mechanisms. We discuss experimental and analytical concerns when referring to studies of two biological reaction mechanisms, namely, transfer of copper ions by the human copper transporter hCtr1 and the mechanism of action of the

Identifiants

pubmed: 36137278
doi: 10.1021/acs.jpcb.2c05235
pmc: PMC9549461
doi:

Substances chimiques

Copper Transport Proteins 0
Ions 0
Transcription Factors 0
Copper 789U1901C5

Types de publication

Journal Article Review Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

7486-7494

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Auteurs

Lukas Hofmann (L)

Department of Chemistry and the Institute of Nanotechnology & Advanced Materials, Faculty of Exact Sciences, Bar-Ilan University, Ramat-Gan 5290002, Israel.

Sharon Ruthstein (S)

Department of Chemistry and the Institute of Nanotechnology & Advanced Materials, Faculty of Exact Sciences, Bar-Ilan University, Ramat-Gan 5290002, Israel.

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Classifications MeSH