Y98 Mutation Leads to the Loss of RsfS Anti-Association Activity in


Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
18 Sep 2022
Historique:
received: 19 08 2022
revised: 08 09 2022
accepted: 15 09 2022
entrez: 23 9 2022
pubmed: 24 9 2022
medline: 28 9 2022
Statut: epublish

Résumé

Ribosomal silencing factor S (RsfS) is a conserved protein that plays a role in the mechanisms of ribosome shutdown and cell survival during starvation. Recent studies demonstrated the involvement of RsfS in the biogenesis of the large ribosomal subunit. RsfS binds to the uL14 ribosomal protein on the large ribosomal subunit and prevents its association with the small subunit. Here, we estimated the contribution of RsfS amino acid side chains at the interface between RsfS and uL14 to RsfS anti-association function in

Identifiants

pubmed: 36142845
pii: ijms231810931
doi: 10.3390/ijms231810931
pmc: PMC9503621
pii:
doi:

Substances chimiques

Amino Acids 0
Bacterial Proteins 0
Ribosomal Proteins 0
Sucrose 57-50-1
Biotin 6SO6U10H04

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Government assignment
ID : for FRC Kazan Scientific Center of Russian Academy of Sciences

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Auteurs

Bulat Fatkhullin (B)

Institute of Protein Research, Russian Academy of Science, 142290 Pushchino, Russia.
Department of Integrated Structural Biology, Institute of Genetics and Molecular and Cellular Biology, INSERM, U964, CNRS, UMR7104, University of Strasbourg, 67400 Illkirch Graffenstaden, France.

Alexander Golubev (A)

Laboratory for Structural Analysis of Biomacromolecules, Kazan Scientific Center of Russian Academy of Sciences, 420111 Kazan, Russia.
Laboratory of Structural Biology, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420021 Kazan, Russia.

Natalia Garaeva (N)

Laboratory for Structural Analysis of Biomacromolecules, Kazan Scientific Center of Russian Academy of Sciences, 420111 Kazan, Russia.
Laboratory of Structural Biology, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420021 Kazan, Russia.

Shamil Validov (S)

Laboratory for Structural Analysis of Biomacromolecules, Kazan Scientific Center of Russian Academy of Sciences, 420111 Kazan, Russia.

Azat Gabdulkhakov (A)

Institute of Protein Research, Russian Academy of Science, 142290 Pushchino, Russia.

Marat Yusupov (M)

Department of Integrated Structural Biology, Institute of Genetics and Molecular and Cellular Biology, INSERM, U964, CNRS, UMR7104, University of Strasbourg, 67400 Illkirch Graffenstaden, France.
Laboratory for Structural Analysis of Biomacromolecules, Kazan Scientific Center of Russian Academy of Sciences, 420111 Kazan, Russia.

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Classifications MeSH