CCN1/Integrin α
Animals
Caspases
/ metabolism
Cysteine-Rich Protein 61
/ metabolism
Fatty Acids, Nonesterified
/ metabolism
Hepatocytes
/ metabolism
Inflammasomes
/ metabolism
Integrin alpha5beta1
/ metabolism
Mice
Mice, Obese
NLR Family, Pyrin Domain-Containing 3 Protein
/ genetics
Oleic Acids
/ metabolism
Palmitic Acids
/ metabolism
Pyroptosis
RNA, Small Interfering
/ metabolism
CCN1
NLRP3 inflammasome
hepatocytes
integrin α5β1
pyroptosis
Journal
Nutrients
ISSN: 2072-6643
Titre abrégé: Nutrients
Pays: Switzerland
ID NLM: 101521595
Informations de publication
Date de publication:
19 Sep 2022
19 Sep 2022
Historique:
received:
31
07
2022
revised:
13
09
2022
accepted:
15
09
2022
entrez:
23
9
2022
pubmed:
24
9
2022
medline:
28
9
2022
Statut:
epublish
Résumé
Hyperlipidemia with high blood levels of free fatty acids (FFA) is the leading cause of non-alcoholic steatohepatitis. CCN1 is a secreted matricellular protein that drives various cellular functions, including proliferation, migration, and differentiation. However, its role in mediating FFA-induced pro-inflammatory cell death and its underlying molecular mechanisms have not been characterized. In this study, we demonstrated that CCN1 was upregulated in the livers of obese mice. The increase in FFA-induced CCN1 was evaluated in vitro by treating hepatocytes with a combination of oleic acid and palmitic acid (2:1). Gene silencing using specific small interfering RNAs (siRNA) revealed that CCN1 participated in FFA-induced intracellular lipid accumulation, caspase-1 activation, and hepatocyte pyroptosis. Next, we identified integrin α
Identifiants
pubmed: 36145246
pii: nu14183871
doi: 10.3390/nu14183871
pmc: PMC9505842
pii:
doi:
Substances chimiques
CCN1 protein, mouse
0
Cysteine-Rich Protein 61
0
Fatty Acids, Nonesterified
0
Inflammasomes
0
Integrin alpha5beta1
0
NLR Family, Pyrin Domain-Containing 3 Protein
0
Nlrp3 protein, mouse
0
Oleic Acids
0
Palmitic Acids
0
RNA, Small Interfering
0
Caspases
EC 3.4.22.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : National Natural Science Foundation of China
ID : 81830015
Organisme : National Natural Science Foundation of China
ID : 82070829
Organisme : National Natural Science Foundation of China
ID : 81770497
Organisme : National Natural Science Foundation of China
ID : 91939108
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