Radiometric Assay of NAPE-PLD Activity.

Biological Assay Chromatography, Thin Layer Phosphatidylethanolamines / chemistry Phospholipase D
Anandamide Fatty acid amides N-Acyl-phosphatidylethanolamines-hydrolyzing phospholipase D N-Acylphosphatidylethanolamines N-Palmitoylethanolamine Radioactive assay Thin layer chromatography

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2023
Historique:
entrez: 24 9 2022
pubmed: 25 9 2022
medline: 28 9 2022
Statut: ppublish

Résumé

N-Acyl-phosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D (NAPE-PLD) is a prominent enzyme involved in the biosynthesis of fatty acid amides, a family of bioactive lipids including anandamide as the prototypical member. Here, we describe a NAPE-PLD assay based on radioactive substrates and product separation by thin layer chromatography (TLC).

Identifiants

DOI: 10.1007/978-1-0716-2728-0_18 PMID: 36152190
pubmed: 36152190
doi: 10.1007/978-1-0716-2728-0_18
doi:

Substances chimiques

Phosphatidylethanolamines 0
Phospholipase D EC 3.1.4.4

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

225-232

Informations de copyright

© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Références

Okamoto Y, Morishita J, Tsuboi K et al (2004) Molecular characterization of a phospholipase D generating anandamide and its congeners. J Biol Chem 279:5298–5305
doi: 10.1074/jbc.M306642200
Balvers MG, Verhoeckx KC, Meijerink J et al (2013) Measurement of palmitoylethanolamide and other N-acylethanolamines during physiological andpathological conditions. CNS Neurol Disord Drug Targets 12:23–33
doi: 10.2174/1871527311312010007
Fezza F, Gasperi V, Mazzei C et al (2005) Radiochromatographic assay of N-acyl-phosphatidylethanolamine-specific phospholipase D activity. Anal Biochem 339:113–120
doi: 10.1016/j.ab.2004.12.005
Bisogno T, Maccarrone M (2013) Latest advances in the discovery of fatty acid amide hydrolase inhibitors. Expert Opin Drug Discov 8:509–522
doi: 10.1517/17460441.2013.780021
Tsuboi K, Okamoto Y, Ikematsu N et al (2011) Enzymatic formation of N-acylethanolamines from N-acylethanolamine plasmalogen through N-acylphosphatidylethanolamine-hydrolyzing phospholipase D-dependent and -independent pathways. Biochim Biophys Acta 1811:565–577
doi: 10.1016/j.bbalip.2011.07.009
Gebeh AK, Willets JM, Bari M et al (2013) Elevated anandamide and related N-acylethanolamine levels occur in the peripheral blood of women with ectopic pregnancy and are mirrored by changes in peripheral fatty acid amide hydrolase activity. J Clin Endocrinol Metab 98:1226–1234
doi: 10.1210/jc.2012-3390

Auteurs

Filomena Fezza (F)

Department of Experimental Medicine, Tor Vergata University of Rome, Rome, Italy. filomena.fezza@uniroma2.it.

Nicolina Mastrangelo (N)

Department of Experimental Medicine, Tor Vergata University of Rome, Rome, Italy.

Mauro Maccarrone (M)

Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, L'Aquila, Italy.
European Center for Brain Research/Santa Lucia Foundation IRCCS, Rome, Italy.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Dosage biologique › Radiometric Assay of NAPE-PLD Activity.
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie sur couche mince › Radiometric Assay of NAPE-PLD Activity.
questionsmedicales.fr › Lipides › Lipides membranaires › Phospholipides › Glycérophosphate › Acides phosphatidiques › Glycérophospholipides › Phosphatidyléthanolamine › Radiometric Assay of NAPE-PLD Activity.
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Esterases › Phosphodiesterases › Phospholipases › Phospholipase D › Radiometric Assay of NAPE-PLD Activity.