Western Blotting of the Endocannabinoid System.

Blotting, Western Cannabinoids Collodion Endocannabinoids / metabolism Sodium Dodecyl Sulfate
CB1 cannabinoid receptor (CB1) Epitope tag Fluorescent secondary antibody detection G protein-coupled receptor (GPCR) Gel electrophoresis

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2023
Historique:
entrez: 24 9 2022
pubmed: 25 9 2022
medline: 28 9 2022
Statut: ppublish

Résumé

Measuring protein levels of receptors and enzymes involved in endocannabinoid metabolism is an important step for understanding the distribution, function, and regulation of these components of the endocannabinoid system. A common approach for detecting proteins from complex biological systems is western blotting. In this chapter, we describe a general approach to western blotting protein components of the endocannabinoid system using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and nitrocellulose membranes with a focus on detecting type 1 cannabinoid (CB

Identifiants

DOI: 10.1007/978-1-0716-2728-0_32 PMID: 36152204
pubmed: 36152204
doi: 10.1007/978-1-0716-2728-0_32
doi:

Substances chimiques

Cannabinoids 0
Endocannabinoids 0
Sodium Dodecyl Sulfate 368GB5141J
Collodion 9004-70-0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

385-393

Informations de copyright

© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Auteurs

Jim Wager-Miller (J)

Department of Psychological and Brain Sciences, Indiana University Bloomington, Bloomington, IN, USA.

Ken Mackie (K)

Department of Psychological and Brain Sciences, Indiana University Bloomington, Bloomington, IN, USA. kmackie@indiana.edu.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Dosage immunologique › Immunotransfert › Technique de Western › Western Blotting of the Endocannabinoid System.
questionsmedicales.fr › Composés chimiques organiques › Hydrocarbures › Terpènes › Cannabinoïdes › Western Blotting of the Endocannabinoid System.
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Polymères › Biopolymères › Cellulose › Collodion › Western Blotting of the Endocannabinoid System.
questionsmedicales.fr › Lipides › Acides gras › Endocannabinoïdes › Western Blotting of the Endocannabinoid System.
questionsmedicales.fr › Lipides › Alcools gras › Dodécan-1-ol › Dodécyl-sulfate de sodium › Western Blotting of the Endocannabinoid System.