Histochemical and Immunohistochemical Methods for the Identification of Proteoglycans.
Carbohydrate histochemistry
Extracellular matrix
Formaldehyde-fixed tissues
Histochemistry
Immunohistochemistry
Proteoglycans
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2023
2023
Historique:
entrez:
24
9
2022
pubmed:
25
9
2022
medline:
28
9
2022
Statut:
ppublish
Résumé
Proteoglycans (PGs) are non-fibrillar extracellular matrix (ECM) molecules composed by a protein core and glycosaminoglycan (GAG) chains. These molecules are present in all tissues playing essential structural, biomechanical, and biological roles. In addition, PGs can regulate cell behavior due to their versatility and ability to interact with other ECM molecules, growth factors, and cells. The distribution of PGs can be evaluated by histochemical and immunohistochemical methods. Histochemical methods aimed to provide a useful overview of the presence and distribution pattern of certain groups of PGs. In contrast, immunohistochemical procedures aimed the identification of highly specific target molecules. In this chapter we described Alcian Blue, Safranin O, and Toluidine Blue histochemical methods for the screening of PGs in tissue sections. Finally, we describe the immunohistochemical procedures for specific identification of PGs (decorin, biglycan, and versican) in formaldehyde-fixed and paraffin-embedded tissues.
Identifiants
pubmed: 36152244
doi: 10.1007/978-1-0716-2675-7_7
doi:
Substances chimiques
Biglycan
0
Chondroitin Sulfate Proteoglycans
0
Decorin
0
Extracellular Matrix Proteins
0
Glycosaminoglycans
0
Versicans
126968-45-4
Tolonium Chloride
15XUH0X66N
Formaldehyde
1HG84L3525
Alcian Blue
P4448TJR7J
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
85-98Informations de copyright
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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