Cholesterol promotes clustering of PI(4,5)P2 driving unconventional secretion of FGF2.
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
07 11 2022
07 11 2022
Historique:
received:
21
06
2021
revised:
22
07
2022
accepted:
30
08
2022
entrez:
29
9
2022
pubmed:
30
9
2022
medline:
4
10
2022
Statut:
ppublish
Résumé
FGF2 is a cell survival factor involved in tumor-induced angiogenesis that is secreted through an unconventional secretory pathway based upon direct protein translocation across the plasma membrane. Here, we demonstrate that both PI(4,5)P2-dependent FGF2 recruitment at the inner plasma membrane leaflet and FGF2 membrane translocation into the extracellular space are positively modulated by cholesterol in living cells. We further revealed cholesterol to enhance FGF2 binding to PI(4,5)P2-containing lipid bilayers. Based on extensive atomistic molecular dynamics (MD) simulations and membrane tension experiments, we proposed cholesterol to modulate FGF2 binding to PI(4,5)P2 by (i) increasing head group visibility of PI(4,5)P2 on the membrane surface, (ii) increasing avidity by cholesterol-induced clustering of PI(4,5)P2 molecules triggering FGF2 oligomerization, and (iii) increasing membrane tension facilitating the formation of lipidic membrane pores. Our findings have general implications for phosphoinositide-dependent protein recruitment to membranes and explain the highly selective targeting of FGF2 toward the plasma membrane, the subcellular site of FGF2 membrane translocation during unconventional secretion of FGF2.
Identifiants
pubmed: 36173379
pii: 213511
doi: 10.1083/jcb.202106123
pmc: PMC9526255
pii:
doi:
Substances chimiques
Lipid Bilayers
0
Phosphatidylinositol 4,5-Diphosphate
0
Fibroblast Growth Factor 2
103107-01-3
Cholesterol
97C5T2UQ7J
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : SFB/TRR 83
Organisme : Bundesministerium für Bildung und Forschung
Organisme : Max Planck Society
Organisme : Sigrid Juselius Foundation
Organisme : Academy of Finland
Organisme : Human Frontier Science Program
ID : RGP0059/2019
Organisme : Helsinki Institute of Life Science
Organisme : Czech Science Foundation
ID : 19-26854X
Organisme : HPC-EUROPA3
ID : INFRAIA-2016-1-730897
Organisme : H2020 Programme
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2022 Lolicato et al.
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