In vivo generation of bone marrow from embryonic stem cells in interspecies chimeras.
blastocyst complementation
bone marrow
interspecies chimeras
irradiation
mice
rat
rats
regenerative medicine
stem cells
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
30 Sep 2022
30 Sep 2022
Historique:
received:
18
09
2021
accepted:
29
09
2022
pubmed:
1
10
2022
medline:
21
10
2022
entrez:
30
9
2022
Statut:
epublish
Résumé
Generation of bone marrow (BM) from embryonic stem cells (ESCs) promises to accelerate the development of future cell therapies for life-threatening disorders. However, such approach is limited by technical challenges to produce a mixture of functional BM progenitor cells able to replace all hematopoietic cell lineages. Herein, we used blastocyst complementation to simultaneously produce BM cell lineages from mouse ESCs in a rat. Based on fluorescence-activated cell sorting analysis and single-cell RNA sequencing, mouse ESCs differentiated into multiple hematopoietic and stromal cell types that were indistinguishable from normal mouse BM cells based on gene expression signatures and cell surface markers. Receptor-ligand interactions identified
Identifiants
pubmed: 36178184
doi: 10.7554/eLife.74018
pii: 74018
pmc: PMC9578712
doi:
pii:
Substances chimiques
CD47 Antigen
0
Ligands
0
Banques de données
GEO
['GSE184940']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NHLBI NIH HHS
ID : R01 HL149631
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL152973
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL158659
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL084151
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL123490
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL141174
Pays : United States
Informations de copyright
© 2022, Wen et al.
Déclaration de conflit d'intérêts
BW, GW, EL, OK, ZT, SD, TK, VK No competing interests declared
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