MT1 Melatonin Receptor Reconstitution in Nanodiscs.

Detergents / chemistry GTP-Binding Proteins / metabolism Lipids / chemistry Liposomes Melatonin Membranes, Artificial Nanoparticles Receptor, Melatonin, MT1 / genetics
Activation Detergent G protein MT1 receptor Nanodiscs Reconstitution Solubilization

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2022
Historique:
entrez: 30 9 2022
pubmed: 1 10 2022
medline: 5 10 2022
Statut: ppublish

Résumé

A way to study G protein-coupled receptors in a minimal system is to reconstruct artificial membrane mimics, made of detergent and/or of lipids in which the purified receptor is maintained. In particular, it is now possible to generate lipid nanoparticles, such as nanodiscs, in which a single receptor molecule is included. Such objects offer the invaluable potential of studying an isolated receptor stabilized in a finely controlled membrane-like environment to evaluate its pharmacology, its function, and its structure at the molecular level. In this chapter, we detail the different steps from the extraction and isolation of a recombinant MT1 melatonin receptor in detergent, down to its reconstitution into nanodiscs. A G protein activation test is further described in order to exemplify how the functionality of such particles may be investigated.

Identifiants

DOI: 10.1007/978-1-0716-2593-4_21 PMID: 36180690
pubmed: 36180690
doi: 10.1007/978-1-0716-2593-4_21
doi:

Substances chimiques

Detergents 0
Lipid Nanoparticles 0
Lipids 0
Liposomes 0
Membranes, Artificial 0
Receptor, Melatonin, MT1 0
GTP-Binding Proteins EC 3.6.1.-
Melatonin JL5DK93RCL

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

171-178

Informations de copyright

© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Références

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Auteurs

Jean A Boutin (JA)

Pole d'expertise Biotechnologie, Chimie & Biologie, Institut de Recherches Servier, Croissy-sur-Seine, France. ja.boutin.pro@gmail.com.
PHARMADEV (Pharmacochimie et biologie pour le développement), Faculté de Pharmacie, Toulouse, France. ja.boutin.pro@gmail.com.

Christel Logez (C)

Pole d'expertise Biotechnologie, Chimie & Biologie, Institut de Recherches Servier, Croissy-sur-Seine, France.
Plateforme IMPReSs, Laboratoire de Biotechnologie et Signalisation Cellulaire, CNRS, Université de Strasbourg, Illkirch, France.
Bioprocess Research & Development, SANOFI PASTEUR, Marcy l'Etoile, France.

Marjorie Damian (M)

IBMM, Université de Montpellier, CNRS, ENSCM, Montpellier, France.

Renaud Wagner (R)

Plateforme IMPReSs, Laboratoire de Biotechnologie et Signalisation Cellulaire, CNRS, Université de Strasbourg, Illkirch, France.

Jean-Louis Banères (JL)

IBMM, Université de Montpellier, CNRS, ENSCM, Montpellier, France.

Gilles Ferry (G)

Pole d'expertise Biotechnologie, Chimie & Biologie, Institut de Recherches Servier, Croissy-sur-Seine, France.

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Classifications MeSH

questionsmedicales.fr › Technologie, industrie et agriculture › Produits domestiques › Détergents › MT1 Melatonin Receptor Reconstitution in Nanodiscs.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protéines G › MT1 Melatonin Receptor Reconstitution in Nanodiscs.
questionsmedicales.fr › Lipides › MT1 Melatonin Receptor Reconstitution in Nanodiscs.
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomimétiques › Membrane artificielle › Liposomes › MT1 Melatonin Receptor Reconstitution in Nanodiscs.
questionsmedicales.fr › Mélatonine › MT1 Melatonin Receptor Reconstitution in Nanodiscs.
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomimétiques › Membrane artificielle › MT1 Melatonin Receptor Reconstitution in Nanodiscs.
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Nanostructures › Nanoparticules › MT1 Melatonin Receptor Reconstitution in Nanodiscs.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Récepteurs couplés aux protéines G › Récepteur de la mélatonine de type MT1 › MT1 Melatonin Receptor Reconstitution in Nanodiscs.