Nonradioactive In Situ Hybridization of MT1 Melatonin Receptor for the Identification of Melatonin Target Cells.
Digoxigenin
In situ hybridization
Melatonin receptor
Pars tuberalis
Phenotyping
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
30
9
2022
pubmed:
1
10
2022
medline:
5
10
2022
Statut:
ppublish
Résumé
Identifying and phenotyping the target cells of a neuroendocrine messenger is one of the key steps to understand neuroendocrine networks and the physiological action of such messengers. In the absence of reliable antibodies directed against the receptor of a neuroendocrine messenger, detecting the expression of the messenger RNA of this receptor is an important tool to identify the target cells of a neuroendocrine messenger such as melatonin. While radioactive in situ hybridization has a higher sensitivity, nonradioactive in situ hybridization has a much better cellular resolution than radioactive in situ hybridization and is therefore better suited for phenotyping the target cells of melatonin. Here we describe a nonradioactive in situ hybridization protocol with its adaptations to various types of histological preparations. This protocol allowed the phenotyping of melatonin target cells in the pars tuberalis of the adenohypophysis, leading to the discovery of photoperiodic melatonin signaling from the pars tuberalis to the hypothalamus.
Identifiants
pubmed: 36180696
doi: 10.1007/978-1-0716-2593-4_27
doi:
Substances chimiques
RNA, Messenger
0
Receptor, Melatonin, MT1
0
Melatonin
JL5DK93RCL
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
219-241Informations de copyright
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Klosen P, Bienvenu C, Demarteau O et al (2002) The mt1 melatonin receptor and RORbeta receptor are co-localized in specific TSH-immunoreactive cells in the pars tuberalis of the rat pituitary. J Histochem Cytochem 50:1647–1657
doi: 10.1177/002215540205001209
Dardente H, Klosen P, Pevet P et al (2003) MT1 melatonin receptor mRNA expressing cells in the pars tuberalis of the European hamster: effect of photoperiod. J Neuroendocrinol 15:778–786
doi: 10.1046/j.1365-2826.2003.01060.x
Hanon EA, Lincoln GA, Fustin JM et al (2008) Ancestral TSH mechanism signals summer in a photoperiodic mammal. Curr Biol 18:1147–1152
doi: 10.1016/j.cub.2008.06.076
Nakao N, Ono H, Yamamura T et al (2008) Thyrotrophin in the pars tuberalis triggers photoperiodic response. Nature 452:317–322
doi: 10.1038/nature06738
Hanon EA, Routledge K, Dardente H et al (2009) Effect of photoperiod on the thyroid-stimulating hormone neuroendocrine system in the European hamster (Cricetus cricetus). J Neuroendocrinol 22:51–55
doi: 10.1111/j.1365-2826.2009.01937.x
Korf HW (2018) Signaling pathways to and from the hypophysial pars tuberalis, an important center for the control of seasonal rhythms. Gen Comp Endocrinol 258:236–243
doi: 10.1016/j.ygcen.2017.05.011
Vanĕcek J, Pavlík A, Illnerová H (1987) Hypothalamic melatonin receptor sites revealed by autoradiography. Brain Res 435:359–362
Dubocovich ML, Takahashi JS (1987) Use of 2-[125I]iodomelatonin to characterize melatonin binding sites in chicken retina. Proc Natl Acad Sci U S A 84:3916–3920
doi: 10.1073/pnas.84.11.3916
Liu C, Weaver DR, Jin X et al (1997) Molecular dissection of two distinct actions of melatonin on the suprachiasmatic circadian clock. Neuron 19:91–102
doi: 10.1016/S0896-6273(00)80350-5
Weaver DR, Rivkees SA, Reppert SM (1989) Localization and characterization of melatonin receptors in rodent brain by in vitro autoradiography. J Neurosci 9:2581–2590
doi: 10.1523/JNEUROSCI.09-07-02581.1989
Weaver DR, Rivkees SA, Carlson LL et al (1991) Localization of melatonin receptors in mammalian brain. In: Klein DC, Moore RY, Reppert SM (eds) Suprachiasmatic nucleus. The mind’s clock. Oxford University Press, New York/Oxford, pp 289–308
Stankov B, Fraschini F, Reiter RJ (1993) The melatonin receptor: distribution, biochemistry, and pharmacology. In: Yu HS, Reiter RJ (eds) Melatonin: biosynthesis, physiological effects, and clinical applications. CRC Press, Boca Raton/Ann Arbor/London, pp 155–186
Bittman EL (1993) Melatonin binding sites in the central nervous system. Methods Neurosci 11:105–121
doi: 10.1016/B978-0-12-185271-9.50011-3
Masson-Pévet M, George D, Kalsbeek A et al (1994) An attempt to correlate brain areas containing melatonin-binding sites with rhythmic functions: a study in five hibernator species. Cell Tissue Res 278:97–106
doi: 10.1007/BF00305781
Masson-Pévet M, George D, Gauer F et al (1993) Demonstration of melatonin-binding sites in cyclohexylamine-formaldehyde-fixed brain tissues. Cell Tissue Res 274:207–209
doi: 10.1007/BF00328002
Lacoste B, Angeloni D, Dominguez-Lopez S et al (2015) Anatomical and cellular localization of melatonin MT1 and MT2 receptors in the adult rat brain. J Pineal Res 58:397–417
doi: 10.1111/jpi.12224
Waly NE, Hallworth R (2015) Circadian pattern of melatonin MT1 and MT2 receptor localization in the rat suprachiasmatic nucleus. J Circadian Rhythms 13:1–7
doi: 10.5334/jcr.ab
Klosen P, Maessen X, van den Bosch de Aguilar P (1993) PEG-embedding for immunocytochemistry. Application to the analysis of immunoreactivity loss during histological processing. J Histochem Cytochem 41:455–463
doi: 10.1177/41.3.8429209
Watson RE Jr, Wiegand SJ, Clough RW et al (1986) Use of cryoprotectant to maintain long-term peptide immunoreactivity and tissue morphology. Peptides 7:155–159
doi: 10.1016/0196-9781(86)90076-8
Hoffman GE, Le WW (2004) Just cool it! Cryoprotectant anti-freeze in immunocytochemistry and in situ hybridization. Peptides 25:425–431
doi: 10.1016/j.peptides.2004.02.004
Hrabovszky E, Petersen SL (2002) Increased concentrations of radioisotopically-labeled complementary ribonucleic acid probe, dextran sulfate, and dithiothreitol in the hybridization buffer can improve results of in situ hybridization histochemistry. J Histochem Cytochem 50:1389–1400
doi: 10.1177/002215540205001012
Braissant O, Wahli W (1998) A simplified in situ hybridization protocol using non-radioactively labeled probes to detect abundant and rare mRNAs on tissue sections. Biochemica 1:10–16
De Block M, Debrouwer D (1993) RNA-RNA in situ hybridization using digoxigenin-labeled probes: the use of high-molecular-weight polyvinyl alcohol in the alkaline phosphatase indoxyl-nitroblue tetrazolium reaction. Anal Biochem 215:86–89
doi: 10.1006/abio.1993.1558