Ph5GT silencing alters flower color and flavonoids metabolome profile in petunia.
Journal
Physiologia plantarum
ISSN: 1399-3054
Titre abrégé: Physiol Plant
Pays: Denmark
ID NLM: 1256322
Informations de publication
Date de publication:
Sep 2022
Sep 2022
Historique:
revised:
17
09
2022
received:
23
07
2022
accepted:
29
09
2022
pubmed:
5
10
2022
medline:
27
10
2022
entrez:
4
10
2022
Statut:
ppublish
Résumé
Anthocyanins are important pigments in plants and glycosylation plays an important role in the stability of anthocyanins. Anthocyanin 5-O-glucosyltransferase (5GT) can glycosylate anthocyanin at the 5-O-position. Till now, the enzymatic activity characteristics of 5GT had been studied in vitro in a variety of plants. However, the subcellular localization of 5GT protein still remained unclear, and little genetic evidence on the roles of 5GT in plants has been reported. The full-length Ph5GT gene from petunia (Petunia hybrida) was isolated in this study. Green fluorescent fusion protein assays revealed that Ph5GT protein was localized to the cytoplasm. Ph5GT was found to be highly expressed in flowers, with highest levels of expression occurring during the coloring stage of flower development. Furthermore, Ph5GT silencing led to the change in flower color from purple to light purple and a significant reduction in total anthocyanin content. The metabolome analysis revealed that the content of malvidins and petunidins modified by glycosylation at the 5-O-position was significantly reduced, while the content of their precursor without glycosylation was significantly increased, implying that Ph5GT could glycosylate malvidin and petunidin derivatives and that the substrate types of Ph5GT were expanded in comparison to previous studies.
Substances chimiques
Anthocyanins
0
Flavonoids
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13795Subventions
Organisme : National Key Research and Development Plan
ID : 2018YFD1000407
Organisme : National Natural Science Foundation of China
ID : 31770737
Organisme : National Natural Science Foundation of China
ID : 31870692
Organisme : National Natural Science Foundation of China
ID : 32271939
Informations de copyright
© 2022 Scandinavian Plant Physiology Society.
Références
Bustin, S.A., Benes, V., Garson, J.A., Hellemans, J., Huggett, J., Kubista, M. et al. (2009) The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clinical Chemistry, 55(4), 611-622. https://doi.org/10.1373/clinchem.2008.112797
Chen, C.J., Chen, H., Zhang, Y., Thomas, H.R., Frank, M.H., He, Y.H. et al. (2020) TBtools: an integrative toolkit developed for interactive analyses of big biological data. Molecular Plant, 13, 1194-1202. https://doi.org/10.1016/j.molp.2020.06.009
Chen, G., Liu, H., Wei, Q., Zhao, H., Liu, J. & Yu, Y. (2017) The acyl-activating enzyme PhAAE13 is an alternative enzymatic source of precursors for anthocyanin biosynthesis in petunia flowers. Journal of Experimental Botany, 68(3), 457-467.
Chen, W., Gong, L., Guo, Z.L., Wang, W.S., Zhang, H.Y., Liu, X.Q. et al. (2013) A novel integrated method for large-scale detection, identification, and quantification of widely targeted metabolites: application in the study of rice metabolomics. Molecular Plant, 6, 1769-1780. https://doi.org/10.1093/mp/sst080
Falcone Ferreyra, M.L., Rius, S.P. & Casati, P. (2012) Flavonoids: biosynthesis, biological functions, and biotechnological applications. Frontiers in Plant Science, 3, 222. https://doi.org/10.3389/fpls.2012.00222
Fedoroff, N.V., Furtek, D.B. & Nelson, O.E. (1984) Cloning of the bronze locus in maize by a simple and generalizable procedure using the transposable controlling element Activator (Ac). Proceedings of the National Academy of Sciences of the United States of America, 81(12), 3825-3829. https://doi.org/10.1073/pnas.81.12.3825
Gachon, C.M.M., Langlois-Meurinne, M. & Saindrenan, P. (2005) Plant secondary metabolism glycosyltransferases: the emerging functional analysis. Trends in Plant Science, 10(11), 542-549. https://doi.org/10.1016/j.tplants.2005.09.007
He, F., Chen, W.K., Yu, K.J., Ji, X.N., Duan, C.Q., Reeves, M.J. et al. (2015) Molecular and biochemical characterization of the UDP-glucose: anthocyanin 5-O-glucosyltransferase from Vitis amurensis. Phytochemistry, 117, 363-372. https://doi.org/10.1016/j.phytochem.2015.06.023
Jonsson, L.M.V., Aarsman, M.E.G, van Diepen, J., de Vlaming, P., Smit, N. & Schram, A.W. (1984). Properties and genetic control of anthocyanin 5-O-glucosyltransferase in flowers of Petunia hybrida. Planta, 160, 341-347.
Ju, Z.G., Sun, W., Meng, X.Y., Liang, L.J., Li, Y.Q., Zhou, T.T. et al. (2018) Isolation and functional characterization of two 5-O-glucosyltransferases related to anthocyanin biosynthesis from Freesia hybrida. Plant Cell, Tissue and Organ Culture, 135(1), 99-110. https://doi.org/10.1007/s11240-018-1447-0
Kang, X.F., Mikami, R. & Akita, Y. (2021) Characterization of 5-O-glucosyltransferase involved in anthocyanin biosynthesis in Cyclamen purpurascens. Plant Biotechnology, 38(2), 263-268. https://doi.org/10.5511/plantbiotechnology.21.0308a
Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35, 1547-1549. https://doi.org/10.1093/molbev/msy096
Le Roy, J., Huss, B., Creach, A., Hawkins, S. & Neutelings, G. (2016) Glycosylation is a major regulator of phenylpropanoid availability and biological activity in plants. Frontiers in Plant Science, 7, 735.
Li, X.J., Zhang, J.Q., Wu, Z.C., Lai, B., Huang, X.M., Qin, Y.H. et al. (2016) Functional characterization of a glucosyltransferase gene, LcUFGT1, involved in the formation of cyanidin glucoside in the pericarp of Litchi chinensis. Physiologia Plantarum, 156(2), 139-149. https://doi.org/10.1111/ppl.12391
Liu, J., Li, J., Wang, H., Fu, Z., Liu, J. & Yu, Y. (2011) Identification and expression analysis of ERF transcription factor genes in petunia during flower senescence and in response to hormone treatments. Journal of Experimental Botany, 62(2), 825-840. https://doi.org/10.1093/jxb/erq324
Liu, J.X., Chang, X.L., Ding, B.B., Zhong, S., Peng, L., Wei, Q. et al. (2019) PhDHS is involved in chloroplast development in petunia. Frontiers in Plant Science, 10, 284.
Locatelli, F., Vannini, C., Magnani, E., Coraggio, I. & Bracale, M. (2003) Efficiency of transient transformation in tobacco protoplasts is independent of plasmid amount. Plant Cell Reports, 21, 865-871. https://doi.org/10.1007/s00299-003-0593-x
Mallona, I., Lischewski, S., Weiss, J., Hause, B. & Egea-Cortines, M. (2010) Validation of reference genes for quantitative real-time PCR during leaf and flower development in Petunia hybrida. BMC Plant Biology, 10, 4.
Meng, X., Li, Y., Zhou, T., Sun, W., Shan, X., Gao, X. et al. (2019) Functional differentiation of duplicated flavonoid 3-O-glycosyltransferases in the flavonol and anthocyanin biosynthesis of Freesia hybrida. Frontiers in Plant Science, 10, 1330.
Nakatsuka, T., Sato, K., Takahashi, H., Yamamura, S. & Nishihara, M. (2008) Cloning and characterization of the UDP-glucose: anthocyanin 5-O-glucosyltransferase gene from blue-flowered gentian. Journal of Experimental Botany, 59, 1241-1252.
Ogata, J., Sakamoto, T., Yamaguchi, M., Kawanobu, S. & Yoshitam, K. (2001) Isolation and characterization of anthocyanin 5-O-glucosyltransferase from flowers of Dahlia variabilis. Journal of Plant Physiology, 158(6), 709-714.
Spitzer-Rimon, B., Farhi, M., Albo, B., Cna'ani, A., Ben Zvi, M.M., Masci, T. et al. (2012) The R2R3-MYB-like regulatory factor EOBI, acting downstream of EOBII, regulates scent production by activating ODO1 and structural scent-related genes in petunia. Plant Cell, 24(12), 5089-5105.
Tan, Y.Y., Liu, J.X., Huang, F., Guan, J.F., Zhong, S., Tang, N. et al. (2014) PhGRL2 protein, interacting with PhACO1, is involved in flower senescence in the petunia. Molecular Plant, 7(8), 1384-1387. https://doi.org/10.1093/mp/ssu024
Tanaka, Y., Sasaki, N. & Ohmiya, A. (2008) Biosynthesis of plant pigments: anthocyanins, betalains and carotenoids. Plant Journal, 54(4), 733-749. https://doi.org/10.1111/j.1365-313X.2008.03447.x
Teusch, M., Forkmann, G. & Seyffert, W. (1986) Genetic control of UDP-glucose: anthocyanin 5-O-glucosyltransferase from flowers of Matthiola incana r.Br. Planta, 168(4), 586-591. https://doi.org/10.1007/BF00392280
Vogt, T. & Jones, P. (2000) Glycosyltransferases in plant natural product synthesis: characterization of a supergene family. Trends in Plant Science, 5(9), 380-386. https://doi.org/10.1016/S1360-1385(00)01720-9
Vogt, T. (2010) Phenylpropanoid biosynthesis. Molecular Plant, 3(1), 2-20. https://doi.org/10.1093/mp/ssp106
Winkel, B.S. (2004) Metabolic channeling in plants. Annual Review Plant Biology, 55, 85-107. https://doi.org/10.1146/annurev.arplant.55.031903.141714
Winkel-Shirley, B. (2001) Flavonoid biosynthesis. A colorful model for genetics, biochemistry, cell biology, and biotechnology. Plant Physiology, 126, 485-493. https://doi.org/10.1104/pp.126.2.485
Yabuya, T., Yamaguchi, M., Imayama, T., Katoh, K. & Ino, I. (2002) Anthocyanin 5-O-glucosyltransferase in flowers of Iris ensata. Plant Science, 162(5), 779-784. https://doi.org/10.1016/S0168-9452(02)00021-3
Yamazaki, M., Yamagishi, E., Gong, Z., Fukuchi-Mizutani, M., Fukui, Y., Tanaka, Y. et al. (2002) Two flavonoid glucosyltransferases from Petunia hybrida: molecular cloning, biochemical properties and developmentally regulated expression. Plant Molecular Biology, 48(4), 401-411. https://doi.org/10.1023/A:1014043214943
Yang, H., Li, Y.F., Cao, Y.W., Shi, W.Q., Xie, E., Mu, N. et al. (2022) Nitrogen nutrition contributes to plant fertility by affecting meiosis initiation. Nature Communications, 13, 485.
Yang, W., Cai, Y., Hu, L., Wei, Q., Chen, G., Bai, M. et al. (2017) PhCESA3 silencing inhibits elongation and stimulates radial expansion in petunia. Scientific Reports, 7, 41471.
Yang, Y.Z., Labate, J.A., Liang, Z.C., Cousins, P., Prins, B., Preece, J.E. et al. (2014) Multiple loss-of-function 5-O-glucosyltransferase alleles revealed in Vitis vinifera, but not in other Vitis species. Theoretical and Applied Genetics, 127(11), 2433-2451. https://doi.org/10.1007/s00122-014-2388-6
Zhao, C.L., Yu, Y.Q., Chen, Z.J., Wen, G.S., Wei, F.G., Zheng, Q. et al. (2017) Stability-increasing effects of anthocyanin glycosyl acylation. Food Chemistry, 214, 119-128. https://doi.org/10.1016/j.foodchem.2016.07.073
Zhao, D.Q., Han, C.X., Ge, J.T. & Tao, J. (2012) Isolation of a UDP-glucose: flavonoid 5-O-glucosyltransferase gene and expression analysis of anthocyanin biosynthetic genes in herbaceous peony (Paeonia lactiflora pall.). Electronic Journal of Biotechnology, 15(6), 7.