Methodologies for Detecting Quantal Exocytosis in Adrenal Chromaffin Cells Through Diamond-Based MEAs.

Chromaffin cells Diamond microelectrode devices Exocytosis Ion beam lithography

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2023
Historique:
entrez: 7 10 2022
pubmed: 8 10 2022
medline: 12 10 2022
Statut: ppublish

Résumé

Diamond-based multiarray sensors are suitable to detect in real-time exocytosis and action potentials from cultured, spontaneously firing chromaffin cells, primary hippocampal neurons, and midbrain dopaminergic neurons. Here, we focus on how amperometric measurements of catecholamine release are performed on micrographitic diamond multiarrays (μG-D-MEAs) with high temporal and spatial resolution by 16 electrodes simultaneously.

Identifiants

pubmed: 36205897
doi: 10.1007/978-1-0716-2671-9_15
doi:

Substances chimiques

Catecholamines 0
Cysteamine 5UX2SD1KE2
Diamond 7782-40-3

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

213-221

Informations de copyright

© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Références

Neher E (2018) Neurosecretion: what can we learn from chromaffin cells. Pflugers Arch Eur J Physiol 470:7–11. https://doi.org/10.1007/S00424-017-2051-6
doi: 10.1007/S00424-017-2051-6
Neher E (2006) A comparison between exocytic control mechanisms in adrenal chromaffin cells and a glutamatergic synapse. Pflugers Arch Eur J Physiol 453:261–268. https://doi.org/10.1007/S00424-006-0143-9
doi: 10.1007/S00424-006-0143-9
Carbone E, Borges R, Eiden LE et al (2019) Chromaffin cells of the adrenal medulla: physiology, pharmacology, and disease. Compr Physiol 9:1443–1502. https://doi.org/10.1002/CPHY.C190003
doi: 10.1002/CPHY.C190003 pubmed: 31688964
Camacho M, Montesinos MS, Machado JD, Borges R (2003) Exocytosis as the mechanism for neural communication. A view from chromaffin cells. Rev Neurol 36:355–360. https://doi.org/10.33588/rn.3604.2003034
doi: 10.33588/rn.3604.2003034 pubmed: 12599135
Sombers LA, Hanchar HJ, Colliver TL et al (2004) The effects of vesicular volume on secretion through the fusion pore in Exocytotic release from PC12 cells. J Neurosci 24:303–309. https://doi.org/10.1523/JNEUROSCI.1119-03.2004
doi: 10.1523/JNEUROSCI.1119-03.2004 pubmed: 14724228 pmcid: 6729980
Mellander LJ, Trouillon R, Svensson MI, Ewing AG (2012) Amperometric post spike feet reveal most exocytosis is via extended kiss-and-run fusion. Sci Rep 2:907. https://doi.org/10.1038/SREP00907
doi: 10.1038/SREP00907 pubmed: 23205269 pmcid: 3510463
Wightman RM, Schroeder TJ, Finnegan JM et al (1995) Time course of release of catecholamines from individual vesicles during exocytosis at adrenal medullary cells. Biophys J 68:383. https://doi.org/10.1016/S0006-3495(95)80199-2
doi: 10.1016/S0006-3495(95)80199-2 pubmed: 7711264 pmcid: 1281698
Picollo F, Gatto Monticone D, Olivero P et al (2012) Fabrication and electrical characterization of three-dimensional graphitic microchannels in single crystal diamond. New J Phys 14:053011. https://doi.org/10.1088/1367-2630/14/5/053011
doi: 10.1088/1367-2630/14/5/053011
Picollo F, Battiato A, Bernardi E et al (2016) All-carbon multi-electrode array for real-time in vitro measurements of oxidizable neurotransmitters. Sci Rep 61(6):1–8. https://doi.org/10.1038/srep20682
doi: 10.1038/srep20682
Meunier A, Fulcrand R, Darchen F et al (2012) Indium tin oxide devices for amperometric detection of vesicular release by single cells. Biophys Chem 162:14–21. https://doi.org/10.1016/J.BPC.2011.12.002
doi: 10.1016/J.BPC.2011.12.002 pubmed: 22257976
Chen X, Gao Y, Hossain M et al (2007) Controlled on-chip stimulation of quantal catecholamine release from chromaffin cells using photolysis of caged Ca2+ on transparent indium-tin-oxide microchip electrodes. Lab Chip 8:161–169. https://doi.org/10.1039/B715308M
doi: 10.1039/B715308M pubmed: 18094774 pmcid: 2489207
Kim BN, Herbst AD, Kim SJ et al (2013) Parallel recording of neurotransmitters release from chromaffin cells using a 10×10 CMOS IC potentiostat array with on-chip working electrodes. Biosens Bioelectron 41:736–744. https://doi.org/10.1016/J.BIOS.2012.09.058
doi: 10.1016/J.BIOS.2012.09.058 pubmed: 23084756
White KA, Kim BN (2021) Quantifying neurotransmitter secretion at single-vesicle resolution using high-density complementary metal–oxide–semiconductor electrode array. Nat Commun 121(12):1–8. https://doi.org/10.1038/s41467-020-20267-0
doi: 10.1038/s41467-020-20267-0
Walsh TR, Wilson M, Sutton AP (2000) Hydrolysis of the amorphous silica surface. II. Calculation of activation barriers and mechanisms. J Chem Phys 113:9191. https://doi.org/10.1063/1.1320057
doi: 10.1063/1.1320057
Lingle CJ, Martinez-Espinosa PL, Guarina L, Carbone E (2018) Roles of Na+, Ca2+, and K+ channels in the generation of repetitive firing and rhythmic bursting in adrenal chromaffin cells. Pflugers Arch Eur J Physiol 470:39–52. https://doi.org/10.1007/S00424-017-2048-1/FIGURES/3
doi: 10.1007/S00424-017-2048-1/FIGURES/3
Marcantoni A, Carabelli V, Vandael DH et al (2009) PDE type-4 inhibition increases L-type ca(2+) currents, action potential firing, and quantal size of exocytosis in mouse chromaffin cells. Pflugers Arch 457:1093–1110. https://doi.org/10.1007/S00424-008-0584-4
doi: 10.1007/S00424-008-0584-4 pubmed: 18779976
Carabelli V, Giancippoli A, Baldelli P et al (2003) Distinct potentiation of L-type currents and secretion by cAMP in rat chromaffin cells. Biophys J 85:1326–1337. https://doi.org/10.1016/S0006-3495(03)74567-6
doi: 10.1016/S0006-3495(03)74567-6 pubmed: 12885675 pmcid: 1303249
Eiden LE, Emery AC, Zhang L, Smith CB (2018) PACAP signaling in stress: insights from the chromaffin cell. Pflugers Arch Eur J Physiol 470:79–88. https://doi.org/10.1007/S00424-017-2062-3/FIGURES/2
doi: 10.1007/S00424-017-2062-3/FIGURES/2
García AG, García-De-Diego AM, Gandía L et al (2006) Calcium signaling and exocytosis in adrenal chromaffin cells. Physiol Rev 86:1093–1131. https://doi.org/10.1152/PHYSREV.00039.2005/ASSET/IMAGES/LARGE/Z9J0040624130010.JPEG
doi: 10.1152/PHYSREV.00039.2005/ASSET/IMAGES/LARGE/Z9J0040624130010.JPEG pubmed: 17015485
Tomagra G, Picollo F, Battiato A et al (2019) Quantal release of dopamine and action potential firing detected in midbrain neurons by multifunctional diamond-based microarrays. Front Neurosci 13:288. https://doi.org/10.3389/fnins.2019.00288
doi: 10.3389/fnins.2019.00288 pubmed: 31024230 pmcid: 6465646
Tomagra G, Aprà P, Battiato A et al (2019) Micro graphite-patterned diamond sensors: towards the simultaneous in vitro detection of molecular release and action potentials generation from excitable cells. Carbon N Y 152:424–433. https://doi.org/10.1016/j.carbon.2019.06.035
doi: 10.1016/j.carbon.2019.06.035
Kuhn B, Picollo F, Carabelli V, Rispoli G (2020) Advanced real-time recordings of neuronal activity with tailored patch pipettes, diamond multi-electrode arrays and electrochromic voltage-sensitive dyes. Pflügers Arch – Eur J Physiol 4731(473):15–36. https://doi.org/10.1007/S00424-020-02472-4
doi: 10.1007/S00424-020-02472-4
Picollo F, Battiato A, Bernardi E et al (2017) Microelectrode arrays of diamond-insulated graphitic channels for real-time detection of Exocytotic events from cultured chromaffin cells and slices of adrenal glands. Anal Chem 88:7493–7499. https://doi.org/10.1021/ACS.ANALCHEM.5B04449
doi: 10.1021/ACS.ANALCHEM.5B04449
Tomagra G, Battiato A, Bernardi E et al (2019) Diamond-based multi electrode arrays for monitoring neurotransmitter release. In: Lecture notes in electrical engineering, vol 539. Springer, Cham, pp 125–134. https://doi.org/10.1007/978-3-030-04324-7_17
doi: 10.1007/978-3-030-04324-7_17
Picollo F, Gosso S, Vittone E et al (2013) A new diamond biosensor with integrated graphitic microchannels for detecting quantal exocytic events from chromaffin cells. Adv Mater 25:4696–4700. https://doi.org/10.1002/adma.201300710
doi: 10.1002/adma.201300710 pubmed: 23847004
Olivero P, Rubanov S, Reichart P et al (2016) Characterization of three-dimensional microstructures in single crystal diamond. Diam Relat Mater 15:1614–1621. https://doi.org/10.1016/j.diamond.2006.01.018
doi: 10.1016/j.diamond.2006.01.018
Uzan-Saguy C, Cytermann C, Brener R et al (1995) Damage threshold for ion-beam induced graphitization of diamond. Appl Phys Lett 67:1194. https://doi.org/10.1063/1.115004
doi: 10.1063/1.115004
Ziegler JF, Ziegler MD, Biersack JP (2010) SRIM – the stopping and range of ions in matter. Nucl Instr Methods Phys Res Sect B Beam Interact Mater Atoms 268:1818–1823. https://doi.org/10.1016/J.NIMB.2010.02.091
doi: 10.1016/J.NIMB.2010.02.091
Tomagra G, Franchino C, Pasquarelli A et al (2019) Simultaneous multisite detection of quantal release from PC12 cells using micro graphitic-diamond multi electrode arrays. Biophys Chem 253:106241. https://doi.org/10.1016/j.bpc.2019.106241
doi: 10.1016/j.bpc.2019.106241 pubmed: 31398633
Gosso S, Turturici M, Franchino C et al (2014) Heterogeneous distribution of exocytotic microdomains in adrenal chromaffin cells resolved by high-density diamond ultra-microelectrode arrays. J Physiol 592:3215–3230. https://doi.org/10.1113/JPHYSIOL.2014.274951
doi: 10.1113/JPHYSIOL.2014.274951 pubmed: 24879870 pmcid: 4146371
Moro M, López M, Gandía L et al (1990) Separation and culture of living adrenaline-and noradrenaline-containing cells from bovine adrenal medullae. Anal Biochem 185(2):243–248. https://doi.org/10.1016/0003-2697(90)90287-J
doi: 10.1016/0003-2697(90)90287-J pubmed: 2339781

Auteurs

Giulia Tomagra (G)

Department of Drug Science and Technology and "NIS" Inter-departmental Centre, University of Torino, Turin, Italy. giulia.tomagra@unito.it.

Claudio Franchino (C)

Department of Drug Science and Technology, University of Torino, Turin, Italy.

Emilio Carbone (E)

Department of Drug Science and Technology and "NIS" Inter-departmental Centre, University of Torino, Turin, Italy.

Andrea Marcantoni (A)

Department of Drug Science and Technology and "NIS" Inter-departmental Centre, University of Torino, Turin, Italy.

Alberto Pasquarelli (A)

Institute of Electron Devices and Circuits, University of Ulm, Ulm, Germany.

Federico Picollo (F)

Department of Physics and "NIS" Inter-departmental Centre, University of Torino, Istituto Nazionale di Fisica Nucleare - Sezione di Torino, Turin, Italy.

Valentina Carabelli (V)

Department of Drug Science and Technology and "NIS" Inter-departmental Centre, University of Torino, Turin, Italy.

Articles similaires

High-throughput Bronchus-on-a-Chip system for modeling the human bronchus.

Akina Mori, Marjolein Vermeer, Lenie J van den Broek et al.
1.00
Humans Bronchi Lab-On-A-Chip Devices Epithelial Cells Goblet Cells
Humans Circadian Rhythm Adult Aged Aging
Humans Chondrocytes Osteoarthritis Matrix Metalloproteinase 13 Drug Discovery
Animals Astrocytes Amyloid beta-Protein Precursor Mice Mice, Transgenic

Classifications MeSH