Molecular epidemiology and genetic characterization of Clostridium perfringens infections in lambs.
Clostridium perfringens
Multiplex PCR
Phylogenic analysis
Sequencing
Journal
Microbial pathogenesis
ISSN: 1096-1208
Titre abrégé: Microb Pathog
Pays: England
ID NLM: 8606191
Informations de publication
Date de publication:
Dec 2022
Dec 2022
Historique:
received:
28
09
2022
revised:
07
10
2022
accepted:
07
10
2022
pubmed:
12
10
2022
medline:
7
12
2022
entrez:
11
10
2022
Statut:
ppublish
Résumé
Clostridium perfringens is gram positive bacterium, wide spread in environment causing various diseases in animals and human. The current study was conducted to indentify the genetic identity of C. perfringens isolates from lambs from Egypt. Using specific primers amplifying genes associated to the toxins produced by C. perfringens, multiplex PCR was used to confirm C. perfringens in 87 out of 140 samples were collected from diseased and suspected lambs. The isolates were classified as type A in 49.4%, type B in 31.1% and type D in 19.5% of isolates. The phylogenetic analysis for the partial sequences of C. perfringens strains based on plc gene, cpb gene and etx gene obtained in the present study showed high degree of similarity with other sequences of C. perfringens strains in GenBank, isolating from sheep from Egypt and other countries. According to the findings, lambs with enterotoxaemia more frequently have C. perfringens type A and an efficient hygienic control program is necessary to reduce the infection spreading among susceptible animals.
Identifiants
pubmed: 36220398
pii: S0882-4010(22)00435-1
doi: 10.1016/j.micpath.2022.105822
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
105822Informations de copyright
Copyright © 2022 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing interest There are no conflicts of interest declared by the authors.