The coilin N-terminus mediates multivalent interactions between coilin and Nopp140 to form and maintain Cajal bodies.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
12 10 2022
12 10 2022
Historique:
received:
24
07
2021
accepted:
16
09
2022
entrez:
12
10
2022
pubmed:
13
10
2022
medline:
15
10
2022
Statut:
epublish
Résumé
Cajal bodies (CBs) are ubiquitous nuclear membraneless organelles (MLOs) that concentrate and promote efficient biogenesis of snRNA-protein complexes involved in splicing (snRNPs). Depletion of the CB scaffolding protein coilin disperses snRNPs, making CBs a model system for studying the structure and function of MLOs. Although it is assumed that CBs form through condensation, the biomolecular interactions responsible remain elusive. Here, we discover the unexpected capacity of coilin's N-terminal domain (NTD) to form extensive fibrils in the cytoplasm and discrete nuclear puncta in vivo. Single amino acid mutational analysis reveals distinct molecular interactions between coilin NTD proteins to form fibrils and additional NTD interactions with the nuclear Nopp140 protein to form puncta. We provide evidence that Nopp140 has condensation capacity and is required for CB assembly. From these observations, we propose a model in which coilin NTD-NTD mediated assemblies make multivalent contacts with Nopp140 to achieve biomolecular condensation in the nucleus.
Identifiants
pubmed: 36224177
doi: 10.1038/s41467-022-33434-2
pii: 10.1038/s41467-022-33434-2
pmc: PMC9556525
doi:
Substances chimiques
Amino Acids
0
RNA, Small Nuclear
0
Ribonucleoproteins, Small Nuclear
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
6005Subventions
Organisme : NINDS NIH HHS
ID : F31 NS105379
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS128358
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA200147
Pays : United States
Informations de copyright
© 2022. The Author(s).
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