Self-collected versus medic-collected sampling for human papillomavirus testing among women in Lagos, Nigeria: a comparative study.
Alphapapillomavirus
Cross-Sectional Studies
Early Detection of Cancer
/ methods
Female
Humans
Middle Aged
Nigeria
Papillomaviridae
/ genetics
Papillomavirus Infections
/ diagnosis
Sensitivity and Specificity
Specimen Handling
/ methods
Uterine Cervical Neoplasms
/ diagnosis
Vaginal Smears
/ methods
beta-Globins
Accuracy
HPV
Medic-sampling
PCR
Self-sampling
Sensitivity
Specificity
Journal
BMC public health
ISSN: 1471-2458
Titre abrégé: BMC Public Health
Pays: England
ID NLM: 100968562
Informations de publication
Date de publication:
15 10 2022
15 10 2022
Historique:
received:
18
04
2022
accepted:
12
09
2022
entrez:
15
10
2022
pubmed:
16
10
2022
medline:
19
10
2022
Statut:
epublish
Résumé
To evaluate the feasibility and performance of self-collected vaginal swab samples for HPV screening among women in Lagos, Nigeria. A cross-sectional study was implemented from March to August 2020 among sexually active women. Study participants provided same-day paired vaginal swab samples. Medic-sampling and poster-directed self-sampling methods were used to collect the two samples per participant. A real-time PCR assay detected HPV 16, HPV 18, other-high-risk (OHR) HPV, and the human β-globin gene. The self-collected samples' sensitivity, specificity, and accuracy were determined against the medic-collected samples using the MedCalc Online Diagnostic Calculator. Of the 213 women aged 16 ~ 63-year-old recruited, 187 (88%) participants had concordant results, while 26 (12%) participants had discordant results. Among the 187 concordant results, 35 (19%) were HPV positive, 150 (80%) participants were HPV negative, and two (1%) were invalid. 18 (69%) out of the 26 discordant samples were invalid. The self-collected sample was invalid for 14 (54%) participants. Two (8%) medic-collected samples were invalid. Compared to the medic-collected sample, the self-collected sample was 89.80% (95% CI: 77.77 ~ 96.60%) sensitive and 98.21% (95% CI: 94.87 ~ 99.63%) specific, with an accuracy of 96.31% (95% CI: 92.87 ~ 98.40%). The mean age for HPV positive and negative participants were 39 and 40, respectively, with an ANOVA p-value of 0.3932. The stratification of HPV infection by the age group was not statistically significant (P > 0.05). With high accuracy of 96%, self-collected sampling is adequate when tested with real-time PCR and may increase the uptake of HPV testing. Though more self-collected samples were invalid than medic-collected samples, most likely due to poor collection, they could be identified for repeat testing. Future implementation can avoid this error with improved guidance and awareness.
Identifiants
pubmed: 36243709
doi: 10.1186/s12889-022-14222-5
pii: 10.1186/s12889-022-14222-5
pmc: PMC9569041
doi:
Substances chimiques
beta-Globins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1922Investigateurs
Mfon Victoria Sunday
(MV)
Janet Fayemi
(J)
Hannah Mfon Udoh
(HM)
Mayokun Omidiji
(M)
Oluwatobi Ogundepo
(O)
Victor Ogbolu
(V)
Informations de copyright
© 2022. The Author(s).
Références
BMJ. 2018 Dec 5;363:k4823
pubmed: 30518635
Cancer Lett. 2020 Feb 28;471:88-102
pubmed: 31812696
Curr Probl Cancer. 2018 Sep;42(5):457-465
pubmed: 30064936
Lancet. 2020 Feb 22;395(10224):575-590
pubmed: 32007141
Front Public Health. 2018 Apr 09;6:77
pubmed: 29686981
Interdiscip Perspect Infect Dis. 2020 Oct 29;2020:8838317
pubmed: 33178264
Infect Agent Cancer. 2017 Feb 6;12:11
pubmed: 28184239
Pan Afr Med J. 2018 Jun 12;30:110
pubmed: 30364362
BMC Public Health. 2019 Jul 31;19(1):1026
pubmed: 31366402
J Oncol. 2019 Oct 10;2019:3257939
pubmed: 31687023
Microorganisms. 2019 Jul 13;7(7):
pubmed: 31337018
PLoS One. 2020 Nov 10;15(11):e0241781
pubmed: 33170891
BMJ Glob Health. 2019 May 14;4(3):e001351
pubmed: 31179035
BMC Womens Health. 2021 Jan 6;21(1):12
pubmed: 33407355
BMC Cancer. 2020 May 26;20(1):473
pubmed: 32456679
Virol J. 2020 Nov 16;17(1):179
pubmed: 33198743
Int J Gynaecol Obstet. 2018 Sep;142(3):359-364
pubmed: 29856071
BMJ Open. 2016 Jan 11;6(1):e008533
pubmed: 26754174
Arch Basic Appl Med. 2018 Feb;6(1):105-112
pubmed: 29905313
BMC Cancer. 2020 Jul 13;20(1):645
pubmed: 32660432
Int J Gynaecol Obstet. 2019 Dec;147(3):332-338
pubmed: 31489627
Infect Agent Cancer. 2020 Oct 14;15:60
pubmed: 33072178
Oncol Lett. 2020 Sep;20(3):2058-2074
pubmed: 32782524
Best Pract Res Clin Obstet Gynaecol. 2020 May;65:98-108
pubmed: 32291178
J Prev Med Hyg. 2012 Dec;53(4):213-9
pubmed: 23469591