Angiotensin II type-1 receptor-associated protein interacts with transferrin receptor-1 and promotes its internalization.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
17 10 2022
17 10 2022
Historique:
received:
06
07
2022
accepted:
13
10
2022
entrez:
17
10
2022
pubmed:
18
10
2022
medline:
20
10
2022
Statut:
epublish
Résumé
Kidney fibrosis is a common pathway that leads to chronic kidney disease. Angiotensin II type-1 receptor (AT1R)-associated protein (ATRAP) was originally identified as an AT1R-binding protein. Previously, we reported that systemic knockout of ATRAP exacerbates kidney fibrosis in aged mice. Although these effects of ATRAP appeared to be AT1R-independent actions, the molecular mechanism remains poorly understood. To elucidate the molecular mechanism of ATRAP independent of AT1R, we explored novel ATRAP-interacting proteins. Mass spectrometric analysis of the immunoprecipitants of a Flag-tagged ATRAP complex revealed 376 candidate proteins that potentially interact with ATRAP. Gene ontology analysis revealed that proteins related to vesicle trafficking, membrane transport, and many membrane proteins, including transferrin receptor 1 (TfR1), were enriched. Because TfR1 promotes cellular iron uptake and iron is a key factor involved in kidney fibrosis, we focused on TfR1 and confirmed that it interacts with ATRAP. In addition, our findings revealed that enhanced ATRAP expression decreased cell-surface TfR1 expression without altering the overall cellular TfR1 expression levels. Furthermore, enhanced ATRAP expression attenuated cellular iron levels. Together, our results highlight the role of ATRAP as a suppressor of TfR1 that functions by facilitating TfR1 internalization, which affects iron metabolism and oxidative stress signaling.
Identifiants
pubmed: 36253401
doi: 10.1038/s41598-022-22343-5
pii: 10.1038/s41598-022-22343-5
pmc: PMC9576747
doi:
Substances chimiques
Adaptor Proteins, Signal Transducing
0
Angiotensin II
11128-99-7
Iron
E1UOL152H7
Receptor, Angiotensin, Type 1
0
Receptors, Transferrin
0
Agtrap protein, mouse
0
Tfrc protein, mouse
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
17376Commentaires et corrections
Type : ErratumIn
Informations de copyright
© 2022. The Author(s).
Références
Toxicology. 2011 May 10;283(2-3):65-87
pubmed: 21414382
Chem Biol. 2008 Jul 21;15(7):647-53
pubmed: 18635001
J Am Heart Assoc. 2017 Jul 27;6(8):
pubmed: 28751545
Kidney Int. 1994 Apr;45(4):1006-13
pubmed: 8007569
Metallomics. 2017 Oct 18;9(10):1367-1375
pubmed: 28671201
Hypertens Res. 2015 Jul;38(7):463-70
pubmed: 25693854
STAR Protoc. 2020 Aug 11;1(2):100083
pubmed: 33111116
Biochim Biophys Acta Gen Subj. 2019 Sep;1863(9):1398-1409
pubmed: 31229492
Cardiovasc Res. 2016 Jun 1;110(3):359-70
pubmed: 27015675
Hum Gene Ther. 2011 Feb;22(2):166-76
pubmed: 20825282
Am J Physiol Renal Physiol. 2010 Nov;299(5):F991-F1003
pubmed: 20739392
J Biol Chem. 2005 Apr 1;280(13):12536-41
pubmed: 15668245
Biochem Soc Trans. 2012 Apr;40(2):451-6
pubmed: 22435829
Adv Exp Med Biol. 2019;1165:585-604
pubmed: 31399986
Pharmacol Rev. 2007 Sep;59(3):251-87
pubmed: 17878513
J Biol Chem. 2019 Apr 26;294(17):7025-7036
pubmed: 30833328
Biochem J. 2011 Oct 1;439(1):97-111
pubmed: 21728994
PLoS One. 2014 Feb 19;9(2):e89355
pubmed: 24586712
Nephrology (Carlton). 2012 May;17(4):311-21
pubmed: 22288610
Genes Cells. 2011 Apr;16(4):448-66
pubmed: 21392187
J Am Heart Assoc. 2013 Jul 31;2(4):e000312
pubmed: 23902639
Kidney Int. 2014 Sep;86(3):570-81
pubmed: 24694992
Antioxidants (Basel). 2022 Apr 27;11(5):
pubmed: 35624725
Free Radic Biol Med. 2019 Mar;133:46-54
pubmed: 29969719
Traffic. 2011 Oct;12(10):1432-43
pubmed: 21718402
PLoS One. 2013 Jul 23;8(7):e70199
pubmed: 23894616
Mol Biol Cell. 2021 Jan 15;32(2):98-108
pubmed: 33236955
Nephrol Dial Transplant. 2001 Apr;16(4):765-70
pubmed: 11274271
Iran J Kidney Dis. 2015 May;9(3):165-79
pubmed: 25957419
Anal Chem. 1996 Mar 1;68(5):850-8
pubmed: 8779443
J Hypertens. 2013 Jun;31(6):1203-13
pubmed: 23552122
Hypertension. 2007 Nov;50(5):926-32
pubmed: 17875818
Annu Rev Physiol. 2018 Feb 10;80:309-326
pubmed: 29068765
Sci Rep. 2019 Nov 12;9(1):16550
pubmed: 31719572
Int J Biochem Cell Biol. 2002 Jan;34(1):93-102
pubmed: 11733189
Neurosci Lett. 2020 Jan 18;716:134684
pubmed: 31830506
Hypertension. 2020 Feb;75(2):413-421
pubmed: 31838906
Mol Biol Cell. 2003 Dec;14(12):5038-50
pubmed: 12960423
Nat Commun. 2019 Apr 3;10(1):1523
pubmed: 30944313
Sci Rep. 2019 Dec 23;9(1):19622
pubmed: 31873081
Neuropeptides. 2014 Jun;48(3):161-6
pubmed: 24786977
Hypertens Res. 2022 Jan;45(1):32-39
pubmed: 34642449
Hypertens Res. 2010 Jul;33(7):713-21
pubmed: 20431588