Characteristic submucosal alteration in biliary carcinogenesis of pancreaticobiliary maljunction with a focus on inflammasome activation.
M2 macrophage
NLRP3
activated fibroblast
biliary cancer
caspase 1
Journal
Journal of hepato-biliary-pancreatic sciences
ISSN: 1868-6982
Titre abrégé: J Hepatobiliary Pancreat Sci
Pays: Japan
ID NLM: 101528587
Informations de publication
Date de publication:
Apr 2023
Apr 2023
Historique:
revised:
25
08
2022
received:
27
10
2021
accepted:
14
09
2022
medline:
28
4
2023
pubmed:
20
10
2022
entrez:
19
10
2022
Statut:
ppublish
Résumé
This study investigated submucosal alterations in biliary carcinogenesis of pancreaticobiliary maljunction (PBM). Thirty-three patients with PBM (including seven with gallbladder [GB] cancer), four with neither biliary tract cancer nor PBM who underwent pancreaticoduodenectomy (controls), and seven with chronic cholecystitis without PBM were enrolled. Protein expression of α-smooth muscle actin (αSMA), CD68, and CD204 in the GB lamina propria and that of NLRP3 and caspase 1 in the GB epithelium and lamina propria were examined. Compared with the control and cholecystitis groups, αSMA expression was higher in the cancerous part (stroma) of the GB in patients with GB cancer + PBM and in the lamina propria of patients with PBM. The CD204/CD68 ratio in the lamina propria was higher in the PBM group than in the control and cholecystitis groups. NLRP3 and caspase 1 expression in both the lamina propria and epithelium was higher in the PBM than control group. In the PBM group, NLRP3- and caspase 1-positive cells in the lamina propria were located near the epithelium. Activated fibroblasts and M2 macrophages in the GB lamina propria may be associated with biliary carcinogenesis of PBM, possibly through inflammasome activation.
Sections du résumé
BACKGROUND
BACKGROUND
This study investigated submucosal alterations in biliary carcinogenesis of pancreaticobiliary maljunction (PBM).
METHODS
METHODS
Thirty-three patients with PBM (including seven with gallbladder [GB] cancer), four with neither biliary tract cancer nor PBM who underwent pancreaticoduodenectomy (controls), and seven with chronic cholecystitis without PBM were enrolled. Protein expression of α-smooth muscle actin (αSMA), CD68, and CD204 in the GB lamina propria and that of NLRP3 and caspase 1 in the GB epithelium and lamina propria were examined.
RESULTS
RESULTS
Compared with the control and cholecystitis groups, αSMA expression was higher in the cancerous part (stroma) of the GB in patients with GB cancer + PBM and in the lamina propria of patients with PBM. The CD204/CD68 ratio in the lamina propria was higher in the PBM group than in the control and cholecystitis groups. NLRP3 and caspase 1 expression in both the lamina propria and epithelium was higher in the PBM than control group. In the PBM group, NLRP3- and caspase 1-positive cells in the lamina propria were located near the epithelium.
CONCLUSION
CONCLUSIONS
Activated fibroblasts and M2 macrophages in the GB lamina propria may be associated with biliary carcinogenesis of PBM, possibly through inflammasome activation.
Substances chimiques
Inflammasomes
0
Caspase 1
EC 3.4.22.36
NLR Family, Pyrin Domain-Containing 3 Protein
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
462-472Subventions
Organisme : Japan Society for the Promotion of Science KAKENHI
ID : 20K08957
Organisme : Japan Society for the Promotion of Science KAKENHI
ID : 21K08671
Organisme : Taiho Pharmaceutical Co., Ltd.
Informations de copyright
© 2022 Japanese Society of Hepato-Biliary-Pancreatic Surgery.
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