De novo transcriptome assembly of Conium maculatum L. to identify candidate genes for coniine biosynthesis.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
20 10 2022
20 10 2022
Historique:
received:
04
02
2022
accepted:
30
09
2022
entrez:
20
10
2022
pubmed:
21
10
2022
medline:
25
10
2022
Statut:
epublish
Résumé
Poison hemlock (Conium maculatum L.) is a notorious weed containing the potent alkaloid coniine. Only some of the enzymes in the coniine biosynthesis have so far been characterized. Here, we utilize the next-generation RNA sequencing approach to report the first-ever transcriptome sequencing of five organs of poison hemlock: developing fruit, flower, root, leaf, and stem. Using a de novo assembly approach, we derived a transcriptome assembly containing 123,240 transcripts. The assembly is deemed high quality, representing over 88% of the near-universal ortholog genes of the Eudicots clade. Nearly 80% of the transcripts were functionally annotated using a combination of three approaches. The current study focuses on describing the coniine pathway by identifying in silico transcript candidates for polyketide reductase, L-alanine:5-keto-octanal aminotransferase, γ-coniceine reductase, and S-adenosyl-L-methionine:coniine methyltransferase. In vitro testing will be needed to confirm the assigned functions of the selected candidates.
Identifiants
pubmed: 36266299
doi: 10.1038/s41598-022-21728-w
pii: 10.1038/s41598-022-21728-w
pmc: PMC9584964
doi:
Substances chimiques
coniine
C479P32L2D
S-Adenosylmethionine
7LP2MPO46S
Alkaloids
0
Oxidoreductases
EC 1.-
Transaminases
EC 2.6.1.-
Methyltransferases
EC 2.1.1.-
Alanine
OF5P57N2ZX
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
17562Informations de copyright
© 2022. The Author(s).
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