Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus Janusvirus.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
22 10 2022
22 10 2022
Historique:
received:
22
06
2022
accepted:
19
10
2022
entrez:
23
10
2022
pubmed:
24
10
2022
medline:
26
10
2022
Statut:
epublish
Résumé
Streptomycetes are ubiquitous soil bacteria. Here we report the complete and annotated genome sequence and characterization of Streptomyces phage Pablito, isolated from a soil sample in Haarlem, the Netherlands using Streptomyces lividans as host. This phage was able to infect a diverse range of Streptomyces strains, but none belonging to the sister genus Kitasatospora. Phage Pablito has double-stranded DNA with a genome length of 49,581 base pairs encoding 76 putative proteins, of which 26 could be predicted. The presence of a serine integrase protein indicated the lysogenic nature of phage Pablito. The phage remained stable over a wide range of temperatures (25-45 °C) and at pH ≥ 7.0, but lost infectivity at temperatures above 55 °C or when the pH dropped below 6.0. This newly isolated phage is closely related to Streptomyces phage Janus and Hank144 and considered a new species classified in the genus Janusvirus, within the subfamily Arquattrovirinae.
Identifiants
pubmed: 36273096
doi: 10.1038/s41598-022-22784-y
pii: 10.1038/s41598-022-22784-y
pmc: PMC9588064
doi:
Substances chimiques
DNA, Viral
0
Integrases
EC 2.7.7.-
Soil
0
Serine
452VLY9402
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
17785Informations de copyright
© 2022. The Author(s).
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