Medium development and production of carotenoids and exopolysaccharides by the extremophile Rhodothermus marinus DSM16675 in glucose-based defined media.
Carotenoids
Defined medium
Exopolysaccharides
Medium development
Rhodothermus marinus DSM 16675
Thermophile
Journal
Microbial cell factories
ISSN: 1475-2859
Titre abrégé: Microb Cell Fact
Pays: England
ID NLM: 101139812
Informations de publication
Date de publication:
23 Oct 2022
23 Oct 2022
Historique:
received:
19
07
2022
accepted:
12
10
2022
entrez:
24
10
2022
pubmed:
25
10
2022
medline:
26
10
2022
Statut:
epublish
Résumé
The marine thermophilic bacterium Rhodothermus marinus can degrade many polysaccharides which makes it interesting as a future cell factory. Progress using this bacterium has, however, been hampered by limited knowledge on media and conditions for biomass production, often resulting in low cell yields and low productivity, highlighting the need to develop conditions that allow studies of the microbe on molecular level. This study presents development of defined conditions that support growth, combined with evaluation of production of carotenoids and exopolysaccharides (EPSs) by R. marinus strain DSM 16675. Two defined media were initially prepared: one including a low addition of yeast extract (modified Wolfe's medium) and one based on specific components (defined medium base, DMB) to which two amino acids (N and Q), were added. Cultivation trials of R. marinus DSM 16675 in shake flasks, resulted in maximum cell densities (OD A minimal defined medium (DRM) was designed that resulted in reproducible growth and an almost doubled formation of both total carotenoids and EPSs. Such defined conditions, are necessary for systematic studies of metabolic pathways, to determine the specific requirements for growth and fully characterize metabolite production.
Sections du résumé
BACKGROUND
BACKGROUND
The marine thermophilic bacterium Rhodothermus marinus can degrade many polysaccharides which makes it interesting as a future cell factory. Progress using this bacterium has, however, been hampered by limited knowledge on media and conditions for biomass production, often resulting in low cell yields and low productivity, highlighting the need to develop conditions that allow studies of the microbe on molecular level. This study presents development of defined conditions that support growth, combined with evaluation of production of carotenoids and exopolysaccharides (EPSs) by R. marinus strain DSM 16675.
RESULTS
RESULTS
Two defined media were initially prepared: one including a low addition of yeast extract (modified Wolfe's medium) and one based on specific components (defined medium base, DMB) to which two amino acids (N and Q), were added. Cultivation trials of R. marinus DSM 16675 in shake flasks, resulted in maximum cell densities (OD
CONCLUSION
CONCLUSIONS
A minimal defined medium (DRM) was designed that resulted in reproducible growth and an almost doubled formation of both total carotenoids and EPSs. Such defined conditions, are necessary for systematic studies of metabolic pathways, to determine the specific requirements for growth and fully characterize metabolite production.
Identifiants
pubmed: 36274123
doi: 10.1186/s12934-022-01946-7
pii: 10.1186/s12934-022-01946-7
pmc: PMC9590192
doi:
Substances chimiques
Carotenoids
36-88-4
Glucose
IY9XDZ35W2
Culture Media
0
Trace Elements
0
Polysaccharides
0
Amino Acids
0
Vitamins
0
Phosphates
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
220Subventions
Organisme : Novo Nordisk Foundation
ID : NNF18OC0034792
Organisme : Horizon 2020 project SECRETed
ID : 101000794
Commentaires et corrections
Type : ErratumIn
Informations de copyright
© 2022. The Author(s).
Références
Zhu D, Adebisi WA, Ahmad F, Sethupathy S, Danso B, Sun J. Recent development of extremophilic bacteria and their application in biorefinery. Front Bioeng Biotechnol. 2020;8:483–483.
pubmed: 32596215
pmcid: 7303364
doi: 10.3389/fbioe.2020.00483
Sardari RRR, Kulcinskaja E, Ron EYC, Björnsdóttir S, Friðjónsson ÓH, Hreggviðsson GÓ, Karlsson EN. Evaluation of the production of exopolysaccharides by two strains of the thermophilic bacterium Rhodothermus marinus. Carbohydr Polym. 2017;156:1–8.
pubmed: 27842803
doi: 10.1016/j.carbpol.2016.08.062
Ron EYC, Plaza M, Kristjansdottir T, Sardari RRR, Bjornsdottir SH, Gudmundsson S, Hreggvidsson GO, Turner C, van Niel EWJ, Nordberg-Karlsson E. Characterization of carotenoids in Rhodothermus marinus. Microbiologyopen. 2018;7:1.
doi: 10.1002/mbo3.536
Nolan M, Tindall BJ, Pomrenke H, Lapidus A, Copeland A, Glavina Del Rio T, Lucas S, Chen F, Tice H, Cheng JF, Saunders E, Han C, Bruce D, Goodwin L, Chain P, Pitluck S, Ovchinikova G, Pati A, Ivanova N, Mavromatis K, Chen A, Palaniappan K, Land M, Hauser L, Chang YJ, Jeffries CD, Brettin T, Göker M, Bristow J, Eisen JA, Markowitz V, Hugenholtz P, Kyrpides NC, Klenk HP, Detter JC. Complete genome sequence of Rhodothermus marinus type strain (R-10). Stand Genom Sci. 2009;1:283–90.
doi: 10.4056/sigs.46736
Alfredsson G, Kristjansson J, Hjörleifsdottir S, Stetter K. Rhodothermus marinus, gen. nov., sp. nov., a thermophilic, halophilic bacterium from submarine hot springs in Iceland. J Gen Microbiol. 1988;134:299–306.
Bjornsdottir SH, Blondal T, Hreggvidsson GO, Eggertsson G, Petursdottir S, Hjorleifsdottir S, Thorbjarnardottir SH, Kristjansson JK. Rhodothermus marinus: physiology and molecular biology. Extremophiles. 2006;10:1–16.
pubmed: 16075163
doi: 10.1007/s00792-005-0466-z
Kristjansdottir T, Ron EYC, Molins-Delgado D, Fridjonsson OH, Turner C, Bjornsdottir SH, Gudmundsson S, van Niel EWJ, Karlsson EN, Hreggvidsson GO. Engineering the carotenoid biosynthetic pathway in Rhodothermus marinus for lycopene production. Metab Eng Commun. 2020;11: e00140.
pubmed: 32793416
pmcid: 7414004
doi: 10.1016/j.mec.2020.e00140
Ron EYC, Sardari RRR, Anthony R, van Niel EWJ, Hreggvidsson GO, Nordberg-Karlsson E. Cultivation technology development of Rhodothermus marinus DSM 16675. Extremophiles. 2019;23:735–45.
pubmed: 31522265
pmcid: 6801211
doi: 10.1007/s00792-019-01129-0
Muller J, Beckers M, Mussmann N, Bongaerts J, Buchs J. Elucidation of auxotrophic deficiencies of Bacillus pumilus DSM 18097 to develop a defined minimal medium. Microb Cell Fact. 2018;17:106.
pubmed: 29986716
pmcid: 6036677
doi: 10.1186/s12934-018-0956-1
Webb C, Kamat SP. Improving fermentation consistency through better inoculum preparation. World J Microbiol Biotechnol. 1993;9:308–12.
pubmed: 24420032
doi: 10.1007/BF00383069
Blücher A, Karlsson E, Holst O. Substrate-dependent production and some properties of a thermostable, α-galactosidase from Rhodothermus marinus. Biotechnol Lett. 2000;22:663–9.
doi: 10.1023/A:1005627501609
Cordova LT, Cipolla RM, Swarup A, Long CP, Antoniewicz MR. 13C metabolic flux analysis of three divergent extremely thermophilic bacteria: Geobacillus sp. LC300, Thermus thermophilus HB8, and Rhodothermus marinus DSM 4252. Metab Eng. 2017;44:182–90.
pubmed: 29037779
pmcid: 5845442
doi: 10.1016/j.ymben.2017.10.007
Cordova LT, Long CP, Venkataramanan KP, Antoniewicz MR. Complete genome sequence, metabolic model construction and phenotypic characterization of Geobacillus LC300, an extremely thermophilic, fast growing, xylose-utilizing bacterium. Metab Eng. 2015;32:74–81.
pubmed: 26391740
pmcid: 5845450
doi: 10.1016/j.ymben.2015.09.009
Singh V, Haque S, Niwas R, Srivastava A, Pasupuleti M, Tripathi CK. Strategies for fermentation medium optimization: an in-depth review. Front Microbiol. 2016;7:2087.
pubmed: 28111566
Gomes J, Gomes I, Terler K, Gubala N, Ditzelmüller G, Steiner W. Optimisation of culture medium and conditions for α-l-Arabinofuranosidase production by the extreme thermophilic eubacterium Rhodothermus marinus. Enzyme Microb Technol. 2000;27:414–22.
pubmed: 10938421
doi: 10.1016/S0141-0229(00)00229-5
Radchenkova N, Vassilev S, Panchev I, Anzelmo G, Tomova I, Nicolaus B, Kuncheva M, Petrov K, Kambourova M. Production and properties of two novel exopolysaccharides synthesized by a thermophilic bacterium Aeribacillus pallidus 418. Appl Biochem Biotechnol. 2013;171:31–43.
pubmed: 23813407
doi: 10.1007/s12010-013-0348-2
Driskill LE, Kusy K, Bauer MW, Kelly RM. Relationship between glycosyl hydrolase inventory and growth physiology of the hyperthermophile Pyrococcus furiosus on carbohydrate-based media. Appl Environ Microbiol. 1999;65:893–7.
pubmed: 10049838
pmcid: 91119
doi: 10.1128/AEM.65.3.893-897.1999
Kristjansdottir T, Hreggvidsson GO, Stefansson SK, Gudmundsdottir EE, Bjornsdottir SH, Fridjonsson OH, Karlsson EN, Vanhalst J, Reynisson B, Gudmundsson S. A genome-scale metabolic reconstruction provides insight into the metabolism of the thermophilic bacterium Rhodothermus marinus. bioRxiv. 2021. https://doi.org/10.1101/2021.05.17.444423 .
doi: 10.1101/2021.05.17.444423
Dudman WF. Growth and extracellular polysaccharide production by Rhizobium meliloti in defined medium. J Bacteriol. 1964;88:640–5.
pubmed: 14208501
pmcid: 277360
doi: 10.1128/jb.88.3.640-645.1964
Muryanto S. On precipitation of struvite (MgNH
Sutherland IW. Biosynthesis of microbial exopolysaccharides. In: Rose AH, Morris JG, editors. Advances in microbial physiology. Cambridge: Academic Press; 1982. p. 79–150.
Finore I, Di Donato P, Mastascusa V, Nicolaus B, Poli A. Fermentation technologies for the optimization of marine microbial exopolysaccharide production. Mar Drugs. 2014;12:3005–24.
pubmed: 24857960
pmcid: 4052328
doi: 10.3390/md12053005
Adil B, Xiang Q, He M, Wu Y, Asghar MA, Arshad M, Qin P, Gu Y, Yu X, Zhao K, et al. Effect of sodium and calcium on polysaccharide production and the activities of enzymes involved in the polysaccharide synthesis of Lentinus edodes. AMB Express. 2020;10:47.
pubmed: 32170413
pmcid: 7070116
doi: 10.1186/s13568-020-00985-w
Corpe WA. Factors influencing growth and polysaccharide formation by strains of Chromobacterium violaceum. J Bacteriol. 1964;88:1433–7.
pubmed: 14234803
pmcid: 277427
doi: 10.1128/jb.88.5.1433-1441.1964
Richard KL, Kelley BR, Johnson JG. Heme uptake and utilization by gram-negative bacterial pathogens. Front Cell Infect Microbiol. 2019;9:81.
pubmed: 30984629
pmcid: 6449446
doi: 10.3389/fcimb.2019.00081
Caza M, Kronstad JW. Shared and distinct mechanisms of iron acquisition by bacterial and fungal pathogens of humans. Front Cell Infect Microbiol. 2013;3:80.
pubmed: 24312900
pmcid: 3832793
doi: 10.3389/fcimb.2013.00080
Pereira MM, Jones KL, Campos MG, Melo AMP, Saraiva LM, Louro RO, Wittung-Stafshede P, Teixeira M. A ferredoxin from the thermohalophilic bacterium Rhodothermus marinus. Biochim Biophys Acta Proteins Proteom. 2002;1601:1–8.
doi: 10.1016/S1570-9639(02)00406-5
Sepúlveda Cisternas I, Salazar JC, García-Angulo VA. Overview on the bacterial iron-riboflavin metabolic axis. Front Microbiol. 2018;9:1478–1478.
pubmed: 30026736
pmcid: 6041382
doi: 10.3389/fmicb.2018.01478
Duncan-Lowey B, Kranzusch PJ. CBASS phage defense and evolution of antiviral nucleotide signaling. Curr Opin Immunol. 2022;74:156–63.
pubmed: 35123147
doi: 10.1016/j.coi.2022.01.002
Ganz T. Iron in innate immunity: starve the invaders. Curr Opin Immunol. 2009;21:63–7.
pubmed: 19231148
pmcid: 2668730
doi: 10.1016/j.coi.2009.01.011
Javed M, Baghaei-Yazdi N. Nutritional optimization for anaerobic growth of Bacillus steaothermophilus LLD-16. J Radiat Res Appl Sci. 2016;9:170–9.
doi: 10.1016/j.jrras.2015.12.007
Wegkamp A, van Oorschot W, de Vos WM, Smid EJ. Characterization of the role of para-aminobenzoic acid biosynthesis in folate production by Lactococcus lactis. Appl Environ Microbiol. 2007;73:2673.
pubmed: 17308179
pmcid: 1855612
doi: 10.1128/AEM.02174-06
Abbas CA, Sibirny AA. Genetic control of biosynthesis and transport of riboflavin and flavin nucleotides and construction of robust biotechnological producers. Microbiol Mol Biol Rev. 2011;75:321–60.
pubmed: 21646432
pmcid: 3122625
doi: 10.1128/MMBR.00030-10
Solmonson A, DeBerardinis RJ. Lipoic acid metabolism and mitochondrial redox regulation. J Biol Chem. 2018;293:7522–30.
pubmed: 29191830
doi: 10.1074/jbc.TM117.000259
Batista A, Franco C, Mendes M, Coelho A, Pereira M. Subunit composition of Rhodothermus marinus respiratory complex I. Anal Biochem. 2010;407:104–10.
pubmed: 20692224
doi: 10.1016/j.ab.2010.07.038
Pan R, Bai X, Chen J, Zhang H, Wang H. Exploring structural diversity of microbe secondary metabolites using OSMAC strategy: a literature review. Front Microbiol. 2019;10:294.
pubmed: 30863377
pmcid: 6399155
doi: 10.3389/fmicb.2019.00294
Vila E, Hornero-Méndez D, Azziz G, Lareo C, Saravia V. Carotenoids from heterotrophic bacteria isolated from Fildes Peninsula, King George Island, Antarctica. Appl Biotechnol Rep. 2019;21: e00306.
doi: 10.1016/j.btre.2019.e00306
Kirti K, Amita S, Priti S, Kumar AM, Jyoti S. Colorful world of microbes: carotenoids and their applications. Adv Behav Biol. 2014;2014:13.
Lutnaes BF, Strand Å, Pétursdóttir SK, Liaaen-Jensen S. Carotenoids of thermophilic bacteria—Rhodothermus marinus from submarine Icelandic hot springs. Biochem Syst Ecol. 2004;32:455–68.
doi: 10.1016/j.bse.2003.09.005
Tian B, Hua Y. Carotenoid biosynthesis in extremophilic Deinococcus-Thermus bacteria. Trends Microbiol. 2010;18:512–20.
pubmed: 20832321
doi: 10.1016/j.tim.2010.07.007
Petry S, Furlan S, Crepeau MJ, Cerning J, Desmazeaud M. Factors affecting exocellular polysaccharide production by Lactobacillus delbrueckii subsp. bulgaricus grown in a chemically defined medium. Appl Environ Microbiol. 2000;66:3427–31.
pubmed: 10919802
pmcid: 92166
doi: 10.1128/AEM.66.8.3427-3431.2000
Degryse E, Glansdorff N, Pierard A. A comparative analysis of extreme thermophilic bacteria belonging to the genus Thermus. Arch Microbiol. 1978;117:189–96.
pubmed: 678024
doi: 10.1007/BF00402307
Biehler E, Mayer F, Hoffmann L, Krause E, Bohn T. Comparison of 3 spectrophotometric methods for carotenoid determination in frequently consumed fruits and vegetables. J Food Sci. 2010;75:C55–61.
pubmed: 20492150
doi: 10.1111/j.1750-3841.2009.01417.x
Imasheva ES, Balashov SP, Wang JM, Smolensky E, Sheves M, Lanyi JK. Chromophore interaction in xanthorhodopsin–retinal dependence of salinixanthin binding. Photochem Photobiol. 2008;84:977–84.
pubmed: 18399915
pmcid: 2747485
doi: 10.1111/j.1751-1097.2008.00337.x