Stepwise progression of β-selection during T cell development involves histone deacetylation.


Journal

Life science alliance
ISSN: 2575-1077
Titre abrégé: Life Sci Alliance
Pays: United States
ID NLM: 101728869

Informations de publication

Date de publication:
01 2023
Historique:
received: 03 08 2022
revised: 02 10 2022
accepted: 04 10 2022
entrez: 25 10 2022
pubmed: 26 10 2022
medline: 28 10 2022
Statut: epublish

Résumé

During T cell development, the first step in creating a unique T cell receptor (TCR) is genetic recombination of the TCRβ chain. The quality of the new TCRβ is assessed at the β-selection checkpoint. Most cells fail this checkpoint and die, but the coordination of fate at the β-selection checkpoint is not yet understood. We shed new light on fate determination during β-selection using a selective inhibitor of histone deacetylase 6, ACY1215. ACY1215 disrupted the β-selection checkpoint. Characterising the basis for this disruption revealed a new, pivotal stage in β-selection, bookended by up-regulation of TCR co-receptors, CD28 and CD2, respectively. Within this "DN3b

Identifiants

pubmed: 36283704
pii: 6/1/e202201645
doi: 10.26508/lsa.202201645
pmc: PMC9595210
pii:
doi:

Substances chimiques

CD28 Antigens 0
Receptors, Antigen, T-Cell, alpha-beta 0
Histones 0
Histone Deacetylase 6 EC 3.5.1.98
Receptors, Antigen, T-Cell 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2022 Chann et al.

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Auteurs

Anchi S Chann (AS)

Optical Sciences Centre, School of Science, Swinburne University of Technology, Hawthorn, Australia.
Peter MacCallum Cancer Centre, Melbourne, Australia.
Monash Biomedicine Discovery Institute, Monash University, Clayton, Australia.
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Australia.

Mirren Charnley (M)

Optical Sciences Centre, School of Science, Swinburne University of Technology, Hawthorn, Australia.
Peter MacCallum Cancer Centre, Melbourne, Australia.

Lucas M Newton (LM)

Department of Biochemistry and Chemistry, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Australia.

Andrea Newbold (A)

Peter MacCallum Cancer Centre, Melbourne, Australia.
Sir Peter MacCallum Department of Oncology, The University of Melbourne, Melbourne, Australia.

Florian Wiede (F)

Monash Biomedicine Discovery Institute, Monash University, Clayton, Australia.
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Australia.

Tony Tiganis (T)

Monash Biomedicine Discovery Institute, Monash University, Clayton, Australia.
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Australia.

Patrick O Humbert (PO)

Department of Biochemistry and Chemistry, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Australia.
Research Centre for Molecular Cancer Prevention, La Trobe University, Melbourne, Australia.
Department of Biochemistry and Pharmacology, University of Melbourne, Melbourne, Australia.
Department of Clinical Pathology, University of Melbourne, Melbourne, Australia.

Ricky W Johnstone (RW)

Peter MacCallum Cancer Centre, Melbourne, Australia.
Sir Peter MacCallum Department of Oncology, The University of Melbourne, Melbourne, Australia.

Sarah M Russell (SM)

Optical Sciences Centre, School of Science, Swinburne University of Technology, Hawthorn, Australia sarah.russell@petermac.org.
Peter MacCallum Cancer Centre, Melbourne, Australia.
Sir Peter MacCallum Department of Oncology, The University of Melbourne, Melbourne, Australia.

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Classifications MeSH