Rapid reconstruction of neural circuits using tissue expansion and light sheet microscopy.
D. melanogaster
connectomics
image analysis
light sheet microscopy
neuroscience
plasticity
synapse
variability
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
26 Oct 2022
26 Oct 2022
Historique:
received:
20
06
2022
accepted:
25
10
2022
pubmed:
27
10
2022
medline:
16
11
2022
entrez:
26
10
2022
Statut:
epublish
Résumé
Brain function is mediated by the physiological coordination of a vast, intricately connected network of molecular and cellular components. The physiological properties of neural network components can be quantified with high throughput. The ability to assess many animals per study has been critical in relating physiological properties to behavior. By contrast, the synaptic structure of neural circuits is presently quantifiable only with low throughput. This low throughput hampers efforts to understand how variations in network structure relate to variations in behavior. For neuroanatomical reconstruction, there is a methodological gulf between electron microscopic (EM) methods, which yield dense connectomes at considerable expense and low throughput, and light microscopic (LM) methods, which provide molecular and cell-type specificity at high throughput but without synaptic resolution. To bridge this gulf, we developed a high-throughput analysis pipeline and imaging protocol using tissue expansion and light sheet microscopy (ExLLSM) to rapidly reconstruct selected circuits across many animals with single-synapse resolution and molecular contrast. Using
Identifiants
pubmed: 36286237
doi: 10.7554/eLife.81248
pii: 81248
pmc: PMC9651950
doi:
pii:
Banques de données
Dryad
['10.5061/dryad.5hqbzkh8b']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Howard Hughes Medical Institute
Pays : United States
Informations de copyright
© 2022, Lillvis et al.
Déclaration de conflit d'intérêts
JL, HO, XD, IP, TK, JC, KR, CG, AH, KW, JB, DM, LM, BM, SS, BD No competing interests declared, RG, PT is a co-inventor on multiple patents related to expansion microscopy, EB is a co-inventor on multiple patents related to expansion microscopy and is also a co-founder of a company that aims to pursue commercial deployment of expansion microscopy-related technology
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