Basal bodies bend in response to ciliary forces.
Journal
Molecular biology of the cell
ISSN: 1939-4586
Titre abrégé: Mol Biol Cell
Pays: United States
ID NLM: 9201390
Informations de publication
Date de publication:
01 12 2022
01 12 2022
Historique:
pubmed:
27
10
2022
medline:
23
11
2022
entrez:
26
10
2022
Statut:
ppublish
Résumé
Motile cilia beat with an asymmetric waveform consisting of a power stroke that generates a propulsive force and a recovery stroke that returns the cilium back to the start. Cilia are anchored to the cell cortex by basal bodies (BBs) that are directly coupled to the ciliary doublet microtubules (MTs). We find that, consistent with ciliary forces imposing on BBs, bending patterns in BB triplet MTs are responsive to ciliary beating. BB bending varies as environmental conditions change the ciliary waveform. Bending occurs where striated fibers (SFs) attach to BBs and mutants with short SFs that fail to connect to adjacent BBs exhibit abnormal BB bending, supporting a model in which SFs couple ciliary forces between BBs. Finally, loss of the BB stability protein Poc1, which helps interconnect BB triplet MTs, prevents the normal distributed BB and ciliary bending patterns. Collectively, BBs experience ciliary forces and manage mechanical coupling of these forces to their surrounding cellular architecture for normal ciliary beating.
Identifiants
pubmed: 36287828
doi: 10.1091/mbc.E22-10-0468-T
pmc: PMC9727800
doi:
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
ar146Subventions
Organisme : NHLBI NIH HHS
ID : F31 HL147495
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM099820
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM140813
Pays : United States
Références
Nature. 2007 May 3;447(7140):97-101
pubmed: 17450123
Mol Biol Cell. 2012 Dec;23(24):4820-32
pubmed: 23115304
Elife. 2019 Feb 11;8:
pubmed: 30741631
J Cell Sci. 1979 Aug;38:105-23
pubmed: 521458
Curr Opin Cell Biol. 2008 Feb;20(1):48-52
pubmed: 18194854
Cell Motil Cytoskeleton. 1998;39(3):223-32
pubmed: 9519903
Science. 2006 Aug 18;313(5789):944-8
pubmed: 16917055
Biophys J. 2011 Jun 8;100(11):2716-25
pubmed: 21641317
J Cell Sci. 1981 Aug;50:65-77
pubmed: 7320077
Cell Motil Cytoskeleton. 2002 Jul;52(3):151-60
pubmed: 12112143
EMBO J. 2012 Feb 1;31(3):552-62
pubmed: 22157822
Nature. 1998 Jun 18;393(6686):711-4
pubmed: 9641685
New Phytol. 2012 Aug;195(3):526-540
pubmed: 22691130
Cell Motil Cytoskeleton. 2007 Aug;64(8):580-9
pubmed: 17443716
J Cell Biol. 2020 Jan 6;219(1):
pubmed: 31740506
J Cell Biol. 2014 Dec 22;207(6):705-15
pubmed: 25533842
Nat Commun. 2018 Jun 7;9(1):2210
pubmed: 29880810
Cytoskeleton (Hoboken). 2016 May;73(5):233-45
pubmed: 27001352
Biophys J. 2008 Jan 1;94(1):298-305
pubmed: 17872955
Cilia. 2016 Jan 19;5:1
pubmed: 26793300
HFSP J. 2007 Sep;1(3):192-208
pubmed: 19404446
J Cell Biol. 1969 Mar;40(3):716-33
pubmed: 5765762
J R Soc Interface. 2021 Jan;18(174):20200660
pubmed: 33435844
Biochim Biophys Acta. 1982 Dec 22;693(2):335-40
pubmed: 6818990
J Cell Biol. 1972 Aug;54(2):246-65
pubmed: 5064817
J Cell Sci. 2007 Sep 1;120(Pt 17):3075-85
pubmed: 17684060
Mol Biol Cell. 2016 Aug 1;27(15):2394-403
pubmed: 27251062
J Cell Biol. 2011 Jul 25;194(2):165-75
pubmed: 21788366
Methods Mol Biol. 2009;586:227-41
pubmed: 19768433
J Exp Biol. 1995 Jul;198(Pt 7):1469-81
pubmed: 9319369
J Protozool. 1967 Nov;14(4):553-65
pubmed: 5604474
Development. 2013 Aug;140(16):3468-77
pubmed: 23900544
Sci Adv. 2020 Feb 14;6(7):eaaz4137
pubmed: 32110738
Cytoskeleton (Hoboken). 2021 Feb;78(2):36-51
pubmed: 33675288
PLoS Biol. 2020 Mar 12;18(3):e3000640
pubmed: 32163404
J Cell Sci. 2019 Aug 7;132(15):
pubmed: 31243050
Methods Cell Biol. 2010;96:117-41
pubmed: 20869521
Proc Natl Acad Sci U S A. 2016 May 17;113(20):E2784-93
pubmed: 27140605
J Cell Biol. 2016 Nov 21;215(4):457-466
pubmed: 27807131
Mol Biol Cell. 2022 Sep 15;33(11):br18
pubmed: 35767367
Cell Motil Cytoskeleton. 1989;14(3):345-58
pubmed: 2531043
Cell Struct Funct. 1999 Feb;24(1):27-33
pubmed: 10355876
Science. 2018 Apr 27;360(6387):
pubmed: 29700238
Results Probl Cell Differ. 2019;67:3-15
pubmed: 31435789
Cold Spring Harb Perspect Biol. 2017 Apr 3;9(4):
pubmed: 27864314
J Exp Biol. 1965 Aug;43(1):155-69
pubmed: 5894323
J Cell Biol. 1974 Oct;63(1):35-63
pubmed: 4424314
J Struct Biol. 2005 Oct;152(1):36-51
pubmed: 16182563
Phys Rev Lett. 2015 Dec 4;115(23):238101
pubmed: 26684142
Curr Biol. 2015 Aug 17;25(16):2177-83
pubmed: 26234217
J Gen Physiol. 1967 Jul;50(6):Suppl:241-58
pubmed: 6050597
Cilia. 2019 Oct 29;8:2
pubmed: 31673332
Cell Motil Cytoskeleton. 2009 Sep;66(9):721-35
pubmed: 19536829
Front Cell Dev Biol. 2019 Nov 01;7:265
pubmed: 31737631
iScience. 2021 Jul 27;24(8):102915
pubmed: 34430806
Biophys J. 2019 Apr 2;116(7):1292-1304
pubmed: 30878201
Cell. 2012 Jan 20;148(1-2):189-200
pubmed: 22265411
Nat Commun. 2018 Jun 11;9(1):2279
pubmed: 29891944
Cell Motil Cytoskeleton. 2009 Aug;66(8):425-36
pubmed: 18828155
Biophys J. 2004 Dec;87(6):3934-44
pubmed: 15465868
Cilia. 2016 Feb 08;5:6
pubmed: 26862393
Biophys J. 1985 Oct;48(4):633-42
pubmed: 3840393
Biophys J. 1983 Jan;41(1):67-79
pubmed: 6824754
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3092-6
pubmed: 5289252
Int J Syst Evol Microbiol. 2002 Mar;52(Pt 2):297-354
pubmed: 11931142
J Cell Biol. 2007 Nov 19;179(4):575-82
pubmed: 18025299
Proc Natl Acad Sci U S A. 1977 May;74(5):2045-9
pubmed: 266725
J Exp Biol. 1984 Jan;108:205-30
pubmed: 6707571
Dev Cell. 2019 Nov 18;51(4):516-525.e5
pubmed: 31743665
Biophys J. 2016 Jun 21;110(12):2759-2768
pubmed: 27332134
Nat Commun. 2021 Jun 21;12(1):3808
pubmed: 34155206
Bioscience. 2014 Dec 1;64(12):1073-1083
pubmed: 26955066
Cold Spring Harb Perspect Biol. 2017 Aug 1;9(8):
pubmed: 28765157
Curr Biol. 2013 Oct 21;23(20):2016-22
pubmed: 24094853
J Cell Biol. 2016 Aug 29;214(5):571-86
pubmed: 27573463
J Struct Biol. 1996 Jan-Feb;116(1):71-6
pubmed: 8742726
Cytoskeleton (Hoboken). 2016 Nov;73(11):652-669
pubmed: 27712041
Mol Cell Biol. 2005 May;25(10):4129-37
pubmed: 15870283
Nature. 2010 Sep 9;467(7312):218-22
pubmed: 20829795
J Cell Biol. 2009 Dec 14;187(6):905-20
pubmed: 20008567
Cell Motil Cytoskeleton. 1995;31(3):207-14
pubmed: 7585990
Dev Biol. 1995 Jun;169(2):644-61
pubmed: 7781905
J Cell Biol. 2011 Oct 3;195(1):19-26
pubmed: 21949415
J Struct Biol. 1997 Dec;120(3):343-52
pubmed: 9441937