Detecting the PEX Like Domain of Matrix Metalloproteinase-14 (MMP-14) with Therapeutic Conjugated CNTs.
CNT
MMP-14
PEX domain
cancer
electrochemical detection
surface modification
Journal
Biosensors
ISSN: 2079-6374
Titre abrégé: Biosensors (Basel)
Pays: Switzerland
ID NLM: 101609191
Informations de publication
Date de publication:
17 Oct 2022
17 Oct 2022
Historique:
received:
11
08
2022
revised:
05
10
2022
accepted:
13
10
2022
entrez:
27
10
2022
pubmed:
28
10
2022
medline:
29
10
2022
Statut:
epublish
Résumé
Matrix metalloproteinases (MMPs) are essential proteins acting directly in the breakdown of the extra cellular matrix and so in cancer invasion and metastasis. Given its impact on tumor angiogenesis, monitoring MMP-14 provides strategic insights on cancer severity and treatment. In this work, we report a new approach to improve the electrochemical interaction of the MMP-14 with the electrode surface while preserving high specificity. This is based on the detection of the hemopexin (PEX) domain of MMP-14, which has a greater availability with a stable and low-cost commercial molecule, as a recognition element. This molecule, called NSC-405020, is specific of the PEX domain of MMP-14 within the binding pocket. Through the covalent grafting of the NSC-405020 molecule on carbon nanotubes (CNTs), we were able to detect and quantify MMP-14 using electrochemical impedance spectroscopy with a linear range of detection of 10 ng⋅mL
Identifiants
pubmed: 36291022
pii: bios12100884
doi: 10.3390/bios12100884
pmc: PMC9599479
pii:
doi:
Substances chimiques
Matrix Metalloproteinase 14
EC 3.4.24.80
Nanotubes, Carbon
0
Hemopexin
9013-71-2
Matrix Metalloproteinase 1
EC 3.4.24.7
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Fonds de Recherche du Québec - Santé
ID : 262745
Organisme : Natural Sciences and Engineering Research Council
ID : 05884
Références
Front Biosci. 2006 May 01;11:1696-701
pubmed: 16368548
Cancer Res. 2013 Apr 15;73(8):2457-2467
pubmed: 23423981
Sensors (Basel). 2008 Mar 27;8(4):2043-2081
pubmed: 27879810
Cancer Res. 2012 May 1;72(9):2339-49
pubmed: 22406620
Chem Rev. 2016 Aug 24;116(16):9001-90
pubmed: 27428515
Biomarkers. 2011 Nov;16(7):616-28
pubmed: 21988680
Bioelectrochemistry. 2021 Dec;142:107944
pubmed: 34500138
J Leukoc Biol. 2007 Apr;81(4):870-92
pubmed: 17185359
Biosens Bioelectron. 2022 Jul 1;207:114177
pubmed: 35305389
Eur Respir J. 2011 Jul;38(1):191-208
pubmed: 21177845
IUBMB Life. 2006 Oct;58(10):589-96
pubmed: 17050376
J Immunoassay Immunochem. 2002;23(1):49-68
pubmed: 11848101
Angew Chem Int Ed Engl. 2007;46(23):4346-9
pubmed: 17465433
Crit Rev Anal Chem. 2016 Nov;46(6):502-20
pubmed: 26941009
Scand J Gastroenterol. 2007 Dec;42(12):1473-8
pubmed: 17852862
Eur J Med Chem. 2020 May 15;194:112260
pubmed: 32224379
Biochim Biophys Acta. 2006 Mar;1758(3):404-12
pubmed: 16307724
Analyst. 2010 Jun;135(6):1345-50
pubmed: 20358056
Nanomicro Lett. 2017;9(3):25
pubmed: 30393720
Anal Biochem. 2010 Apr 15;399(2):202-10
pubmed: 20060801
J Phys Chem B. 2005 Jun 23;109(24):11925-32
pubmed: 16852469
Clin Cancer Res. 2000 Dec;6(12):4823-30
pubmed: 11156241
J Chromatogr A. 2008 May 2;1189(1-2):417-25
pubmed: 18001757
Nanoscale. 2012 Mar 21;4(6):1948-63
pubmed: 22337209
Sensors (Basel). 2021 Oct 01;21(19):
pubmed: 34640898
Front Oncol. 2019 Dec 06;9:1370
pubmed: 31921634
Bioinorg Chem Appl. 2010;2010:603978
pubmed: 21350599
J Electroanal Chem (Lausanne). 2020 Dec 1;878:114596
pubmed: 32863810
Bioelectrochemistry. 2019 Dec;130:107324
pubmed: 31295697
Folia Histochem Cytobiol. 2010 Jan 1;48(1):101-3
pubmed: 20529824
Medicine (Baltimore). 2020 Feb;99(7):e19224
pubmed: 32049862
Anal Biochem. 2011 Dec 1;419(1):53-60
pubmed: 21864497
Clin Biochem. 2010 Jul;43(10-11):899-904
pubmed: 20380828
Sens Actuators B Chem. 2020 Dec 15;325:128780
pubmed: 32843820
Talanta. 2019 Mar 1;194:548-553
pubmed: 30609571
Circ Res. 2003 May 2;92(8):827-39
pubmed: 12730128
J Enzyme Inhib Med Chem. 2016;31(sup1):177-183
pubmed: 27028474
Eur J Cancer. 2007 Jul;43(11):1764-71
pubmed: 17600697
Cell Mol Life Sci. 1999 Apr;55(4):639-52
pubmed: 10357232
Analyst. 2021 Jun 14;146(12):4066-4079
pubmed: 34048512
Biopreserv Biobank. 2018 Dec;16(6):463-466
pubmed: 30379574
Chem Soc Rev. 2013 Jul 7;42(13):5944-62
pubmed: 23615920
PLoS One. 2015 Aug 13;10(8):e0135544
pubmed: 26270045
PLoS Comput Biol. 2013;9(3):e1002951
pubmed: 23505360
Int J Nanomedicine. 2010 Sep 07;5:639-45
pubmed: 20856839
Nat Rev Cancer. 2002 Mar;2(3):161-74
pubmed: 11990853
Chem Res Toxicol. 2014 Sep 15;27(9):1556-65
pubmed: 25116078
J Control Release. 2017 Aug 10;259:62-75
pubmed: 28153760
Mikrochim Acta. 2022 Feb 8;189(3):94
pubmed: 35132460
Sensors (Basel). 2017 Aug 20;17(8):
pubmed: 28825646
Sci Rep. 2019 Nov 22;9(1):17347
pubmed: 31758050
Small. 2005 Feb;1(2):180-92
pubmed: 17193428
Biosens Bioelectron. 2021 May 15;180:113087
pubmed: 33662844
Talanta. 2019 Dec 1;205:120142
pubmed: 31450394
Front Cell Neurosci. 2020 Jul 28;14:191
pubmed: 32848608
Photodiagnosis Photodyn Ther. 2021 Sep;35:102420
pubmed: 34242818
Onkologie. 2012;35(1-2):49-53
pubmed: 22310347
Cells. 2020 Apr 26;9(5):
pubmed: 32357580
Int J Nanomedicine. 2016 Apr 15;11:1537-47
pubmed: 27143876
Anal Chem. 2012 Feb 7;84(3):1466-73
pubmed: 22242647