The Cytotoxicity and Genotoxicity of Bioactive Dental Materials.

Annexin A5 Fluorescein-5-isothiocyanate Propidium Pulp Capping and Pulpectomy Agents
cytotoxicity dental materials flow cytometry genotoxicity pulp capping vital pulp therapy

Journal

Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052

Informations de publication

Date de publication:
15 10 2022
Historique:
received: 13 09 2022
revised: 01 10 2022
accepted: 12 10 2022
entrez: 27 10 2022
pubmed: 28 10 2022
medline: 29 10 2022
Statut: epublish

Résumé

The promotion of biologically based treatment strategies in restorative dentistry is of paramount importance, as invasive treatments should be avoided to maintain the tooth's vitality. This study aimed to assess the biocompatibility of commercially available bioactive materials that can be used for dental pulp capping. The study was performed with a monocyte/macrophage peripheral blood SC cell line (ATCC CRL-9855) on the following six specific bioactive materials: ProRoot MTA (Dentsply Sirona), MTA Angelus (Angelus), Biodentine (Septodont), TheraCal LC (Bisco), ACTIVA BioACTIVE (Pulpdent) and Predicta Bioactive Bulk (Parkell). The cytotoxicity of the investigated agents was measured using a resazurin-based cell viability assay, while the genotoxicity was evaluated using an alkaline comet assay. Additionally, flow cytometry (FC) apoptosis detection was conducted with a FITC (fluorescein isothiocyanate) Annexin V Apoptosis Detection Kit I. FC cell-cycle arrest assessment was carried out with propidium iodide staining. The results of this study showed no significant cytotoxicity and genotoxicity (

Identifiants

DOI: 10.3390/cells11203238 PMID: 36291107 PMC: PMC9600439
pubmed: 36291107
pii: cells11203238
doi: 10.3390/cells11203238
pmc: PMC9600439
pii:
doi:

Substances chimiques

Annexin A5 0
Fluorescein-5-isothiocyanate I223NX31W9
MTA-Angelus 0
Propidium 36015-30-2
Pulp Capping and Pulpectomy Agents 0
TheraCal 0
tricalcium silicate 404G39282C

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

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Auteurs

Marta Kunert (M)

Department of General Dentistry, Medical University of Lodz, 92-213 Lodz, Poland.
ORCID: 0000-0003-3477-2423

Wioletta Rozpedek-Kaminska (W)

Department of Clinical Chemistry and Biochemistry, Medical University of Lodz, 92-215 Lodz, Poland.
ORCID: 0000-0001-9206-1203

Grzegorz Galita (G)

Department of Clinical Chemistry and Biochemistry, Medical University of Lodz, 92-215 Lodz, Poland.
ORCID: 0000-0002-1559-2796

Salvatore Sauro (S)

Dental Biomaterials and Minimally Invasive Dentistry, Departamento de Odontología, Facultad de Ciencias de la Salud, Universidad CEU-Cardenal Herrera C/Del Pozo ss/n, Alfara del Pa-triarca, 46115 Valencia, Spain.
Department of Therapeutic Dentistry, I. M. Sechenov First Moscow State Medical University, Moscow 119146, Russia.
ORCID: 0000-0002-2527-8776

Rim Bourgi (R)

Department of Restorative Dentistry, School of Dentistry, Saint-Joseph University, Beirut 1107 2180, Lebanon.
ORCID: 0000-0002-8003-2664

Louis Hardan (L)

Department of Restorative Dentistry, School of Dentistry, Saint-Joseph University, Beirut 1107 2180, Lebanon.
ORCID: 0000-0001-8577-2285

Ireneusz Majsterek (I)

Department of Clinical Chemistry and Biochemistry, Medical University of Lodz, 92-215 Lodz, Poland.

Monika Lukomska-Szymanska (M)

Department of General Dentistry, Medical University of Lodz, 92-213 Lodz, Poland.
ORCID: 0000-0002-6110-4298

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de transport › Protéines de liaison au calcium › Annexines › Annexine A5 › The Cytotoxicity and Genotoxicity of Bioactive...
questionsmedicales.fr › Composés polycycliques › Spiranes › Fluorescéines › Fluorescéine-5-isothiocyanate › The Cytotoxicity and Genotoxicity of Bioactive...
questionsmedicales.fr › Composés hétérocycliques › Composés hétérocycliques à cycles fusionnés › Composés hétérocycliques 3 noyaux › Phénanthridines › Propidium › The Cytotoxicity and Genotoxicity of Bioactive...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Matériaux dentaires › Agents de coiffage pulpaire et de pulpectomie › The Cytotoxicity and Genotoxicity of Bioactive...