Enhanced electrochemical measurement of β-galactosidase activity in whole cells by coexpression of lactose permease, LacY.

Escherichia coli / genetics Membrane Transport Proteins beta-Galactosidase / metabolism Galactose Escherichia coli Proteins Monosaccharide Transport Proteins Symporters
Miller assay electrochemical sensing synthetic biology whole-cell β-galactosidase assay

Journal

BioTechniques
ISSN: 1940-9818
Titre abrégé: Biotechniques
Pays: England
ID NLM: 8306785

Informations de publication

Date de publication:
11 2022
Historique:
pubmed: 28 10 2022
medline: 24 11 2022
entrez: 27 10 2022
Statut: ppublish

Résumé

Whole-cell biosensing links the sensing and computing capabilities of microbes to the generation of a detectable reporter. Whole cells enable dynamic biological computation (filtered noise, amplified signals, logic gating etc.). Enzymatic reporters enable

Identifiants

DOI: 10.2144/btn-2022-0090 PMID: 36300351
pubmed: 36300351
doi: 10.2144/btn-2022-0090
doi:

Substances chimiques

lactose permease 9068-45-5
Membrane Transport Proteins 0
beta-Galactosidase EC 3.2.1.23
Galactose X2RN3Q8DNE
Escherichia coli Proteins 0
LacY protein, E coli 0
Monosaccharide Transport Proteins 0
Symporters 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

233-237

Auteurs

Eric VanArsdale (E)

Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
Institute for Bioscience & Biotechnology Research, University of Maryland, College Park, MD 20742, USA.
Fischell Institute for Biomedical Devices, University of Maryland, College Park, MD 20742, USA.

Juliana Pitzer (J)

Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.

Sally Wang (S)

Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
Institute for Bioscience & Biotechnology Research, University of Maryland, College Park, MD 20742, USA.
Fischell Institute for Biomedical Devices, University of Maryland, College Park, MD 20742, USA.

Kristina Stephens (K)

Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
Institute for Bioscience & Biotechnology Research, University of Maryland, College Park, MD 20742, USA.
Fischell Institute for Biomedical Devices, University of Maryland, College Park, MD 20742, USA.

Chen-Yu Chen (CY)

Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
Institute for Bioscience & Biotechnology Research, University of Maryland, College Park, MD 20742, USA.
Fischell Institute for Biomedical Devices, University of Maryland, College Park, MD 20742, USA.

Gregory F Payne (GF)

Institute for Bioscience & Biotechnology Research, University of Maryland, College Park, MD 20742, USA.
Fischell Institute for Biomedical Devices, University of Maryland, College Park, MD 20742, USA.
ORCID: 0000-0001-6638-9459

William E Bentley (WE)

Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
Institute for Bioscience & Biotechnology Research, University of Maryland, College Park, MD 20742, USA.
Fischell Institute for Biomedical Devices, University of Maryland, College Park, MD 20742, USA.
ORCID: 0000-0002-4855-7866

Articles similaires

Bioinspired gelated cell sheet-supported lactobacillus biofilm for aerobic vaginitis diagnosis and treatment.

Yueyue Gui, Qingfei Sun, Kexin Li et al.
1.00
Female Biofilms Animals Lactobacillus Mice

Prediction of strain level phage-host interactions across the Escherichia genus using only genomic information.

Baptiste Gaborieau, Hugo Vaysset, Florian Tesson et al.
1.00
Host Specificity Bacteriophages Genomics Algorithms Escherichia coli

Mutational analysis of Phanerochaete chrysosporium´s purine transporter.

Mariana Barraco-Vega, Manuel Sanguinetti, Gabriela da Rosa et al.
1.00
Phanerochaete Fungal Proteins Purines Aspergillus nidulans DNA Mutational Analysis

Equine bone marrow-derived mesenchymal stromal cells reduce established S. aureus and E. coli biofilm matrix in vitro.

Sarah M Khatibzadeh, Linda A Dahlgren, Clayton C Caswell et al.
1.00
Biofilms Horses Animals Escherichia coli Mesenchymal Stem Cells

Classifications MeSH

questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Enhanced electrochemical measurement of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Enhanced electrochemical measurement of...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Glycosidases › Galactosidases › beta-Galactosidase › Enhanced electrochemical measurement of...
questionsmedicales.fr › Glucides › Sucres › Oses › Hexose › Galactose › Enhanced electrochemical measurement of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Protéines Escherichia coli › Enhanced electrochemical measurement of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Transporteurs de monosaccharides › Enhanced electrochemical measurement of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Pompes ioniques › Symporteurs › Enhanced electrochemical measurement of...