A framework for detecting noncoding rare-variant associations of large-scale whole-genome sequencing studies.


Journal

Nature methods
ISSN: 1548-7105
Titre abrégé: Nat Methods
Pays: United States
ID NLM: 101215604

Informations de publication

Date de publication:
12 2022
Historique:
received: 06 11 2021
accepted: 06 09 2022
pubmed: 28 10 2022
medline: 7 12 2022
entrez: 27 10 2022
Statut: ppublish

Résumé

Large-scale whole-genome sequencing studies have enabled analysis of noncoding rare-variant (RV) associations with complex human diseases and traits. Variant-set analysis is a powerful approach to study RV association. However, existing methods have limited ability in analyzing the noncoding genome. We propose a computationally efficient and robust noncoding RV association detection framework, STAARpipeline, to automatically annotate a whole-genome sequencing study and perform flexible noncoding RV association analysis, including gene-centric analysis and fixed window-based and dynamic window-based non-gene-centric analysis by incorporating variant functional annotations. In gene-centric analysis, STAARpipeline uses STAAR to group noncoding variants based on functional categories of genes and incorporate multiple functional annotations. In non-gene-centric analysis, STAARpipeline uses SCANG-STAAR to incorporate dynamic window sizes and multiple functional annotations. We apply STAARpipeline to identify noncoding RV sets associated with four lipid traits in 21,015 discovery samples from the Trans-Omics for Precision Medicine (TOPMed) program and replicate several of them in an additional 9,123 TOPMed samples. We also analyze five non-lipid TOPMed traits.

Identifiants

pubmed: 36303018
doi: 10.1038/s41592-022-01640-x
pii: 10.1038/s41592-022-01640-x
pmc: PMC10008172
mid: NIHMS1862492
doi:

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1599-1611

Subventions

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Investigateurs

Namiko Abe (N)
Gonçalo Abecasis (G)
Francois Aguet (F)
Christine Albert (C)
Laura Almasy (L)
Alvaro Alonso (A)
Seth Ament (S)
Peter Anderson (P)
Pramod Anugu (P)
Deborah Applebaum-Bowden (D)
Kristin Ardlie (K)
Dan Arking (D)
Allison Ashley-Koch (A)
Stella Aslibekyan (S)
Tim Assimes (T)
Dimitrios Avramopoulos (D)
Najib Ayas (N)
Adithya Balasubramanian (A)
John Barnard (J)
Kathleen Barnes (K)
R Graham Barr (RG)
Emily Barron-Casella (E)
Lucas Barwick (L)
Terri Beaty (T)
Gerald Beck (G)
Diane Becker (D)
Lewis Becker (L)
Rebecca Beer (R)
Amber Beitelshees (A)
Emelia Benjamin (E)
Takis Benos (T)
Marcos Bezerra (M)
Nathan Blue (N)
Russell Bowler (R)
Ulrich Broeckel (U)
Jai Broome (J)
Deborah Brown (D)
Karen Bunting (K)
Esteban Burchard (E)
Carlos Bustamante (C)
Erin Buth (E)
Jonathan Cardwell (J)
Vincent Carey (V)
Julie Carrier (J)
April Carson (A)
Cara Carty (C)
Richard Casaburi (R)
Juan P Casas Romero (JP)
James Casella (J)
Peter Castaldi (P)
Mark Chaffin (M)
Christy Chang (C)
Yi-Cheng Chang (YC)
Daniel Chasman (D)
Sameer Chavan (S)
Bo-Juen Chen (BJ)
Wei-Min Chen (WM)
Yii-Der Ida Chen (YI)
Michael Cho (M)
Seung Hoan Choi (SH)
Lee-Ming Chuang (LM)
Mina Chung (M)
Ren-Hua Chung (RH)
Clary Clish (C)
Suzy Comhair (S)
Elaine Cornell (E)
Carolyn Crandall (C)
James Crapo (J)
Jeffrey Curtis (J)
Brian Custer (B)
Coleen Damcott (C)
Dawood Darbar (D)
Sean David (S)
Colleen Davis (C)
Michelle Daya (M)
Mariza de Andrade (M)
Lisa de Las Fuentes (LL)
Michael DeBaun (M)
Ranjan Deka (R)
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Harsha Doddapaneni (H)
Qing Duan (Q)
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Susan K Dutcher (SK)
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Charles Farber (C)
Jesse Farek (J)
Tasha Fingerlin (T)
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C Charles Gu (CC)
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Yun Ju Sung (YJ)
Jody Sylvia (J)
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Larry Bielak (L)
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Yingchang Lu (Y)
Lisa Martin (L)
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Auteurs

Zilin Li (Z)

Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, MA, USA. li@hsph.harvard.edu.
Department of Biostatistics and Health Data Science, Indiana University School of Medicine, Indianapolis, IN, USA. li@hsph.harvard.edu.

Xihao Li (X)

Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, MA, USA.

Hufeng Zhou (H)

Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, MA, USA.

Sheila M Gaynor (SM)

Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, MA, USA.

Margaret Sunitha Selvaraj (MS)

Center for Genomic Medicine and Cardiovascular Research Center, Massachusetts General Hospital, Boston, MA, USA.
Program in Medical and Population Genetics, Broad Institute of Harvard and MIT, Cambridge, MA, USA.
Department of Medicine, Harvard Medical School, Boston, MA, USA.

Theodore Arapoglou (T)

Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, MA, USA.

Corbin Quick (C)

Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, MA, USA.

Yaowu Liu (Y)

School of Statistics, Southwestern University of Finance and Economics, Chengdu, China.

Han Chen (H)

Human Genetics Center, Department of Epidemiology, Human Genetics, and Environmental Sciences, School of Public Health, The University of Texas Health Science Center at Houston, Houston, TX, USA.
Center for Precision Health, School of Biomedical Informatics, The University of Texas Health Science Center at Houston, Houston, TX, USA.

Ryan Sun (R)

Department of Biostatistics, University of Texas MD Anderson Cancer Center, Houston, TX, USA.

Rounak Dey (R)

Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, MA, USA.

Donna K Arnett (DK)

Dean's Office, University of Kentucky, College of Public Health, Lexington, KY, USA.

Paul L Auer (PL)

Division of Biostatistics, Institute for Health & Equity and Cancer Center, Medical College of Wisconsin, Milwaukee, WI, USA.

Lawrence F Bielak (LF)

Department of Epidemiology, School of Public Health, University of Michigan, Ann Arbor, MI, USA.

Joshua C Bis (JC)

Cardiovascular Health Research Unit, Department of Medicine, University of Washington, Seattle, WA, USA.

Thomas W Blackwell (TW)

Department of Biostatistics and Center for Statistical Genetics, University of Michigan, Ann Arbor, MI, USA.

John Blangero (J)

Department of Human Genetics and South Texas Diabetes and Obesity Institute, School of Medicine, The University of Texas Rio Grande Valley, Brownsville, TX, USA.

Eric Boerwinkle (E)

Human Genetics Center, Department of Epidemiology, Human Genetics, and Environmental Sciences, School of Public Health, The University of Texas Health Science Center at Houston, Houston, TX, USA.
Human Genome Sequencing Center, Baylor College of Medicine, Houston, TX, USA.

Donald W Bowden (DW)

Department of Biochemistry, Wake Forest University School of Medicine, Winston-Salem, NC, USA.

Jennifer A Brody (JA)

Cardiovascular Health Research Unit, Department of Medicine, University of Washington, Seattle, WA, USA.

Brian E Cade (BE)

Program in Medical and Population Genetics, Broad Institute of Harvard and MIT, Cambridge, MA, USA.
Division of Sleep and Circadian Disorders, Brigham and Women's Hospital, Boston, MA, USA.
Division of Sleep Medicine, Harvard Medical School, Boston, MA, USA.

Matthew P Conomos (MP)

Department of Biostatistics, University of Washington, Seattle, WA, USA.

Adolfo Correa (A)

Jackson Heart Study, Department of Medicine, University of Mississippi Medical Center, Jackson, MS, USA.

L Adrienne Cupples (LA)

Department of Biostatistics, Boston University School of Public Health, Boston, MA, USA.
Framingham Heart Study, National Heart, Lung, and Blood Institute and Boston University, Framingham, MA, USA.

Joanne E Curran (JE)

Department of Human Genetics and South Texas Diabetes and Obesity Institute, School of Medicine, The University of Texas Rio Grande Valley, Brownsville, TX, USA.

Paul S de Vries (PS)

Human Genetics Center, Department of Epidemiology, Human Genetics, and Environmental Sciences, School of Public Health, The University of Texas Health Science Center at Houston, Houston, TX, USA.

Ravindranath Duggirala (R)

Department of Human Genetics and South Texas Diabetes and Obesity Institute, School of Medicine, The University of Texas Rio Grande Valley, Brownsville, TX, USA.

Nora Franceschini (N)

Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, NC, USA.

Barry I Freedman (BI)

Department of Internal Medicine, Nephrology, Wake Forest University School of Medicine, Winston-Salem, NC, USA.

Harald H H Göring (HHH)

Department of Human Genetics and South Texas Diabetes and Obesity Institute, School of Medicine, The University of Texas Rio Grande Valley, Brownsville, TX, USA.

Xiuqing Guo (X)

The Institute for Translational Genomics and Population Sciences, Department of Pediatrics, The Lundquist Institute for Biomedical Innovation at Harbor-UCLA Medical Center, Torrance, CA, USA.

Rita R Kalyani (RR)

GeneSTAR Research Program, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Charles Kooperberg (C)

Division of Public Health Sciences, Fred Hutchinson Cancer Center, Seattle, WA, USA.

Brian G Kral (BG)

GeneSTAR Research Program, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Leslie A Lange (LA)

Division of Biomedical Informatics and Personalized Medicine, Department of Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.

Bridget M Lin (BM)

Department of Biostatistics, University of North Carolina, Chapel Hill, NC, USA.

Ani Manichaikul (A)

Center for Public Health Genomics, University of Virginia, Charlottesville, VA, USA.

Alisa K Manning (AK)

Department of Medicine, Harvard Medical School, Boston, MA, USA.
Metabolism Program, The Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Clinical and Translational Epidemiology Unit, Mongan Institute, Massachusetts General Hospital, Boston, MA, USA.

Lisa W Martin (LW)

Division in Cardiology, George Washington School of Medicine and Health Sciences, Washington, DC, USA.

Rasika A Mathias (RA)

GeneSTAR Research Program, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

James B Meigs (JB)

Program in Medical and Population Genetics, Broad Institute of Harvard and MIT, Cambridge, MA, USA.
Department of Medicine, Harvard Medical School, Boston, MA, USA.
Division of General Internal Medicine, Massachusetts General Hospital, Boston, MA, USA.

Braxton D Mitchell (BD)

Department of Medicine, University of Maryland School of Medicine, Baltimore, MD, USA.
Geriatrics Research and Education Clinical Center, Baltimore VA Medical Center, Baltimore, MD, USA.

May E Montasser (ME)

Division of Endocrinology, Diabetes, and Nutrition, Program for Personalized and Genomic Medicine, University of Maryland School of Medicine, Baltimore, MD, USA.

Alanna C Morrison (AC)

Human Genetics Center, Department of Epidemiology, Human Genetics, and Environmental Sciences, School of Public Health, The University of Texas Health Science Center at Houston, Houston, TX, USA.

Take Naseri (T)

Ministry of Health, Government of Samoa, Apia, Samoa.

Jeffrey R O'Connell (JR)

Department of Medicine, University of Maryland School of Medicine, Baltimore, MD, USA.

Nicholette D Palmer (ND)

Department of Biochemistry, Wake Forest University School of Medicine, Winston-Salem, NC, USA.

Patricia A Peyser (PA)

Department of Epidemiology, School of Public Health, University of Michigan, Ann Arbor, MI, USA.

Bruce M Psaty (BM)

Cardiovascular Health Research Unit, Department of Medicine, University of Washington, Seattle, WA, USA.
Department of Epidemiology, University of Washington, Seattle, WA, USA.
Departments of Health Systems and Population Health, University of Washington, Seattle, WA, USA.

Laura M Raffield (LM)

Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Susan Redline (S)

Division of Sleep and Circadian Disorders, Brigham and Women's Hospital, Boston, MA, USA.
Division of Sleep Medicine, Harvard Medical School, Boston, MA, USA.
Division of Pulmonary, Critical Care, and Sleep Medicine, Beth Israel Deaconess Medical Center, Boston, MA, USA.

Alexander P Reiner (AP)

Division of Public Health Sciences, Fred Hutchinson Cancer Center, Seattle, WA, USA.
Department of Epidemiology, University of Washington, Seattle, WA, USA.

Muagututi'a Sefuiva Reupena (MS)

Lutia I Puava Ae Mapu I Fagalele, Apia, Samoa.

Kenneth M Rice (KM)

Department of Biostatistics, University of Washington, Seattle, WA, USA.

Stephen S Rich (SS)

Center for Public Health Genomics, University of Virginia, Charlottesville, VA, USA.

Jennifer A Smith (JA)

Department of Epidemiology, School of Public Health, University of Michigan, Ann Arbor, MI, USA.
Survey Research Center, Institute for Social Research, University of Michigan, Ann Arbor, MI, USA.

Kent D Taylor (KD)

The Institute for Translational Genomics and Population Sciences, Department of Pediatrics, The Lundquist Institute for Biomedical Innovation at Harbor-UCLA Medical Center, Torrance, CA, USA.

Margaret A Taub (MA)

Department of Biostatistics, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.

Ramachandran S Vasan (RS)

Framingham Heart Study, National Heart, Lung, and Blood Institute and Boston University, Framingham, MA, USA.
Department of Medicine, Boston University School of Medicine, Boston, MA, USA.

Daniel E Weeks (DE)

Department of Human Genetics and Biostatistics, University of Pittsburgh, Pittsburgh, PA, USA.

James G Wilson (JG)

Division of Cardiology, Beth Israel Deaconess Medical Center, Boston, MA, USA.

Lisa R Yanek (LR)

GeneSTAR Research Program, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Wei Zhao (W)

Department of Epidemiology, School of Public Health, University of Michigan, Ann Arbor, MI, USA.

Jerome I Rotter (JI)

The Institute for Translational Genomics and Population Sciences, Department of Pediatrics, The Lundquist Institute for Biomedical Innovation at Harbor-UCLA Medical Center, Torrance, CA, USA.

Cristen J Willer (CJ)

Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA.
Department of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI, USA.
Department of Human Genetics, University of Michigan, Ann Arbor, MI, USA.

Pradeep Natarajan (P)

Center for Genomic Medicine and Cardiovascular Research Center, Massachusetts General Hospital, Boston, MA, USA.
Program in Medical and Population Genetics, Broad Institute of Harvard and MIT, Cambridge, MA, USA.
Department of Medicine, Harvard Medical School, Boston, MA, USA.

Gina M Peloso (GM)

Department of Biostatistics, Boston University School of Public Health, Boston, MA, USA.
Framingham Heart Study, National Heart, Lung, and Blood Institute and Boston University, Framingham, MA, USA.

Xihong Lin (X)

Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, MA, USA. xlin@hsph.harvard.edu.
Program in Medical and Population Genetics, Broad Institute of Harvard and MIT, Cambridge, MA, USA. xlin@hsph.harvard.edu.
Department of Statistics, Harvard University, Cambridge, MA, USA. xlin@hsph.harvard.edu.

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