Ursolic acid regulates gut microbiota and corrects the imbalance of Th17/Treg cells in T1DM rats.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2022
2022
Historique:
received:
13
06
2022
accepted:
18
10
2022
entrez:
3
11
2022
pubmed:
4
11
2022
medline:
8
11
2022
Statut:
epublish
Résumé
Ursolic acid (UA), a natural pentacyclic triterpenoid obtained from fruit and several traditional Chinese medicinal plants, exhibits anti-inflammatory and hypoglycemic properties. However, its protective effects against type 1 diabetes mellitus (T1DM) have not been explored. In this study, streptozotocin-induced T1DM rat models were established and treated with UA for six weeks. T1DM rats treated with UA were used to observe the effects of UA on body weight and fasting blood glucose (FBG) levels. Pathological changes in the pancreas were observed using immunohistochemical staining. The gut microbiota distribution was measured using 16S rDNA high-throughput sequencing. The proportions of Th17 and Treg cells were examined using flow cytometry. Protein and mRNA expression of molecules involved in Th17/Treg cell differentiation were assessed by quantitative real-time PCR and western blotting. The correlation between gut microbiota and Th17/Treg cell differentiation in T1DM was analyzed using redundancy analysis (RDA) analysis. Compared with the model group, FBG levels declined, and the progressive destruction of pancreatic β cells was alleviated. The diversity and uniformity of gut microbiota in T1DM rats treated with UA increased significantly. Interestingly, the Th17/Treg cell differentiation imbalance was corrected and positively correlated with the expression of Foxp3 and IL-10, and negatively correlated with the expression of RORγt, IL-17A, and TNF-α. These findings suggest that UA can lower FBG levels in T1DM rats, delay the progressive destruction of pancreatic β-cells, and modulate gut microbiota homeostasis and immune function in streptozotocin-induced T1DM rats.
Identifiants
pubmed: 36327331
doi: 10.1371/journal.pone.0277061
pii: PONE-D-22-16987
pmc: PMC9632920
doi:
Substances chimiques
Streptozocin
5W494URQ81
Triterpenes
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0277061Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Diabetologia. 2016 Mar;59(3):426-35
pubmed: 26780750
Circulation. 2018 Dec 18;138(25):2895-2907
pubmed: 30566007
Diabetes Care. 2017 Jan;40(Suppl 1):S4-S5
pubmed: 27979887
Biochim Biophys Acta. 2006 Oct;1760(10):1505-12
pubmed: 16828971
Molecules. 2020 Nov 25;25(23):
pubmed: 33255782
Dis Markers. 2020 Aug 27;2020:3936247
pubmed: 32908614
Nat Rev Immunol. 2008 Jul;8(7):523-32
pubmed: 18566595
Sci Rep. 2014 Jan 22;4:3814
pubmed: 24448554
Int J Mol Sci. 2018 Mar 03;19(3):
pubmed: 29510522
Biomedicines. 2021 Mar 13;9(3):
pubmed: 33805840
Diabetes. 2011 Nov;60(11):2903-13
pubmed: 21896932
Diabetes Res Clin Pract. 2018 Jul;141:256-263
pubmed: 29733871
World J Gastroenterol. 2021 May 21;27(19):2394-2414
pubmed: 34040330
Diabetes. 2009 Jun;58(6):1302-11
pubmed: 19289457
Int Immunopharmacol. 2009 Jan;9(1):113-9
pubmed: 19013541
J Diabetes Complications. 2020 Feb;34(2):107449
pubmed: 31677982
Naunyn Schmiedebergs Arch Pharmacol. 2017 Nov;390(11):1097-1104
pubmed: 28756460
J Biol Chem. 2008 Jun 20;283(25):17003-8
pubmed: 18434325
Brain Res Bull. 2020 Jul;160:150-161
pubmed: 32147532
Sci Rep. 2018 May 14;8(1):7500
pubmed: 29760423
Am J Transl Res. 2019 Apr 15;11(4):2393-2402
pubmed: 31105845
Acta Pharmacol Sin. 2014 Sep;35(9):1177-87
pubmed: 25087995
Int J Mol Sci. 2021 Apr 27;22(9):
pubmed: 33925641
J Cell Biochem. 2002;86(4):651-64
pubmed: 12210732
J Oleo Sci. 2022 Feb 3;71(2):289-300
pubmed: 35034940
Mol Nutr Food Res. 2017 Aug;61(8):
pubmed: 28218451
Diabetes Metab Res Rev. 2018 Oct;34(7):e3043
pubmed: 29929213
Diabetes Res Clin Pract. 2019 Nov;157:107842
pubmed: 31518658
Clin Immunol. 2004 Sep;112(3):202-9
pubmed: 15308110
Molecules. 2019 Jul 29;24(15):
pubmed: 31362424
Am J Physiol Endocrinol Metab. 2014 May 15;306(10):E1099-109
pubmed: 24644243
N Engl J Med. 2017 Apr 13;376(15):1419-1429
pubmed: 28402773
Nature. 2018 Oct;562(7728):583-588
pubmed: 30356187
Atherosclerosis. 2018 Aug;275:333-341
pubmed: 30015296
Front Immunol. 2020 Sep 03;11:1832
pubmed: 33013834
Lancet. 2016 Jun 4;387(10035):2340-2348
pubmed: 27302273
Front Immunol. 2015 Dec 17;6:639
pubmed: 26734006
Immunology. 2019 May;157(1):70-85
pubmed: 30712258
J Sci Food Agric. 2020 Feb;100(3):986-994
pubmed: 31650545
Physiol Rep. 2022 Mar;10(5):e15151
pubmed: 35274817
Lancet. 2018 Jun 16;391(10138):2449-2462
pubmed: 29916386
Drug Dev Res. 2018 Mar;79(2):70-80
pubmed: 29380400
Front Immunol. 2019 Jul 23;10:1662
pubmed: 31428083
Science. 2011 Jan 21;331(6015):337-41
pubmed: 21205640
Immunity. 2000 Apr;12(4):431-40
pubmed: 10795741
Biosci Rep. 2019 Jun 18;39(6):
pubmed: 31147456
Oxid Med Cell Longev. 2020 Feb 18;2020:8565760
pubmed: 32148658
Diabetes. 2015 May;64(5):1786-93
pubmed: 25510240
Fitoterapia. 2020 Nov;147:104735
pubmed: 33010369
BMC Med. 2013 Feb 21;11:46
pubmed: 23433344
Pharmacol Res. 2015 Aug;98:9-15
pubmed: 25747961
BMC Endocr Disord. 2020 Mar 9;20(1):34
pubmed: 32151244
Biomed Pharmacother. 2019 Oct;118:109393
pubmed: 31545258
Drug Des Devel Ther. 2021 Jun 01;15:2339-2355
pubmed: 34103897
Yonsei Med J. 2004 Aug 31;45(4):703-10
pubmed: 15344213
J Immunol Res. 2020 May 25;2020:6841078
pubmed: 32537469
Front Physiol. 2021 May 28;12:650190
pubmed: 34122127
Neurochem Res. 2017 Feb;42(2):337-346
pubmed: 27734181
Nat Med. 2018 Sep;24(9):1395-1406
pubmed: 30150719
Diabetes. 2019 Feb;68(2):258-265
pubmed: 30665954
Pediatr Diabetes. 2019 Aug;20(5):574-583
pubmed: 31081243
J Neuroimmunol. 2015 Apr 15;281:61-7
pubmed: 25867469
Diabetologia. 2013 Aug;56(8):1773-80
pubmed: 23699989
Hum Immunol. 2013 Jun;74(6):701-7
pubmed: 23395729