Flow cytometry analysis of CD11c-positive peripheral blood mononuclear cells in horses.

Animals Horses Flow Cytometry / veterinary Leukocytes, Mononuclear Toll-Like Receptor 4 CD11c Antigen Antibodies, Monoclonal Dendritic Cells
CD11c Dendritic cells Horse Monoclonal antibody

Journal

Veterinary immunology and immunopathology
ISSN: 1873-2534
Titre abrégé: Vet Immunol Immunopathol
Pays: Netherlands
ID NLM: 8002006

Informations de publication

Date de publication:
Nov 2022
Historique:
received: 16 08 2022
revised: 03 10 2022
accepted: 16 10 2022
pubmed: 4 11 2022
medline: 22 11 2022
entrez: 3 11 2022
Statut: ppublish

Résumé

Horses have played a prominent role in shaping our modern world, with important effects on health. Unfortunately, better characterization of the horse immune system is still needed. In this report, using flow cytometry techniques, four monoclonal antibodies against horse CD11c integrin were characterized and described for their ability to provide a positive recognition signal in peripheral blood mononuclear cells. Further immune cell phenotype experiments were performed using MHC-II, CD14, TLR4 and the specific anti-horse CD11c monoclonal antibody (1C4). With this staining panel, it was possible to detect a cell population defined by CD11c

Identifiants

DOI: 10.1016/j.vetimm.2022.110504 PMID: 36327940
pubmed: 36327940
pii: S0165-2427(22)00124-6
doi: 10.1016/j.vetimm.2022.110504
pii:
doi:

Substances chimiques

Toll-Like Receptor 4 0
CD11c Antigen 0
Antibodies, Monoclonal 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

110504

Informations de copyright

Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.

Auteurs

Mayela Rosario Espinoza-Duarte (MR)

Facultad de Medicina y Ciencias Biomédicas, Autonomous University of Chihuahua, Circuito Universitario s/n. Campus II, 31125 Chihuahua, Chih, Mexico; Laboratorio Nacional de Citometría de Flujo - Sede Chihuahua, Autonomous University of Chihuahua, Faculty of Medicine, Circuito Universitario s/n. Campus II, 31125 Chihuahua, Chih, Mexico.

Carlos Ortega-Ochoa (C)

Facultad de Zootecnia y Ecologia, Periférico Fco. R. Almada km 1, Chihuahua 31453, Mexico.

Alejandro Baca-Ramirez (A)

Facultad de Medicina y Ciencias Biomédicas, Autonomous University of Chihuahua, Circuito Universitario s/n. Campus II, 31125 Chihuahua, Chih, Mexico.

Lourival D Possani (LD)

Instituto de Biotecnología - UNAM, Universidad Nacional Autónoma de México, Av. Universidad 2001, Col. Chamilpa, 62210 Cuernavaca, Morelos, Mexico.

Gerardo P Espino-Solis (GP)

Facultad de Medicina y Ciencias Biomédicas, Autonomous University of Chihuahua, Circuito Universitario s/n. Campus II, 31125 Chihuahua, Chih, Mexico; Laboratorio Nacional de Citometría de Flujo - Sede Chihuahua, Autonomous University of Chihuahua, Faculty of Medicine, Circuito Universitario s/n. Campus II, 31125 Chihuahua, Chih, Mexico. Electronic address: gespinos@uach.mx.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Flow cytometry analysis of CD11c-positive...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Perissodactyla › Equidae › Equus caballus › Flow cytometry analysis of CD11c-positive...
questionsmedicales.fr › Techniques d'investigation › Techniques cytologiques › Cytophotométrie › Cytométrie en flux › Flow cytometry analysis of CD11c-positive...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Agranulocytes › Flow cytometry analysis of CD11c-positive...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Récepteurs immunologiques › Récepteurs de reconnaissance de motifs moléculaires › Récepteurs de type Toll › Récepteur de type Toll-4 › Flow cytometry analysis of CD11c-positive...
questionsmedicales.fr › Facteurs biologiques › Antigènes › Antigènes de surface › Molécules d'adhérence cellulaire › Intégrine alphaX bêta2 › Antigènes CD11c › Flow cytometry analysis of CD11c-positive...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Anticorps monoclonaux › Flow cytometry analysis of CD11c-positive...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Système phagocytaire mononucléé › Cellules dendritiques › Flow cytometry analysis of CD11c-positive...