Longitudinal analysis of the Five Sisters hot springs in Yellowstone National Park reveals a dynamic thermoalkaline environment.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
04 11 2022
04 11 2022
Historique:
received:
13
07
2022
accepted:
07
10
2022
pubmed:
6
11
2022
medline:
9
11
2022
entrez:
5
11
2022
Statut:
epublish
Résumé
Research focused on microbial populations of thermoalkaline springs has been driven in a large part by the lure of discovering functional enzymes with industrial applications in high-pH and high temperature environments. While several studies have focused on understanding the fundamental ecology of these springs, the small molecule profiles of thermoalkaline springs have largely been overlooked. To better understand how geochemistry, small molecule composition, and microbial communities are connected, we conducted a three-year study of the Five Sisters (FS) springs that included high-resolution geochemical measurements, 16S rRNA sequencing of the bacterial and archaeal community, and mass spectrometry-based metabolite and extracellular small molecule characterization. Integration of the four datasets facilitated a comprehensive analysis of the interwoven thermoalkaline spring system. Over the course of the study, the microbial population responded to changing environmental conditions, with archaeal populations decreasing in both relative abundance and diversity compared to bacterial populations. Decreases in the relative abundance of Archaea were associated with environmental changes that included decreased availability of specific nitrogen- and sulfur-containing extracellular small molecules and fluctuations in metabolic pathways associated with nitrogen cycling. This multi-factorial analysis demonstrates that the microbial community composition is more closely correlated with pools of extracellular small molecules than with the geochemistry of the thermal springs. This is a novel finding and suggests that a previously overlooked component of thermal springs may have a significant impact on microbial community composition.
Identifiants
pubmed: 36333441
doi: 10.1038/s41598-022-22047-w
pii: 10.1038/s41598-022-22047-w
pmc: PMC9636164
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Nitrogen
N762921K75
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
18707Subventions
Organisme : National Science Foundation
ID : CHE1852214
Organisme : NIGMS NIH HHS
ID : P20 GM103474
Pays : United States
Organisme : NIH HHS
ID : S10 OD028650
Pays : United States
Informations de copyright
© 2022. The Author(s).
Références
Curr Opin Microbiol. 2015 Jun;25:136-45
pubmed: 26113243
Mol Biol Evol. 2013 Apr;30(4):772-80
pubmed: 23329690
PLoS One. 2013;8(1):e53350
pubmed: 23326417
Front Microbiol. 2013 Nov 12;4:330
pubmed: 24282404
ISME J. 2014 Jun;8(6):1166-74
pubmed: 24430481
PLoS Comput Biol. 2017 Nov 3;13(11):e1005752
pubmed: 29099853
Nat Microbiol. 2020 Nov;5(11):1428-1438
pubmed: 32807893
Bioinform Biol Insights. 2019 Oct 17;13:1177932219882961
pubmed: 31666793
J Chem Inf Model. 2013 May 24;53(5):1223-8
pubmed: 23480664
Microb Ecol. 2020 Apr;79(3):770-784
pubmed: 31432245
Nat Microbiol. 2016 Feb 01;1:15032
pubmed: 27572438
Environ Microbiol. 2016 May;18(5):1403-14
pubmed: 26271760
Appl Environ Microbiol. 2009 Apr;75(8):2464-75
pubmed: 19218404
ISME J. 2016 Jan;10(1):210-24
pubmed: 26140529
Extremophiles. 2013 Jul;17(4):649-62
pubmed: 23708551
Nat Biotechnol. 2012 Oct;30(10):918-20
pubmed: 23051804
Nature. 2017 Nov 23;551(7681):457-463
pubmed: 29088705
Front Microbiol. 2016 Sep 27;7:1521
pubmed: 27729905
Appl Environ Microbiol. 2022 Jan 11;88(1):e0095821
pubmed: 34669438
Proc Natl Acad Sci U S A. 2017 Jun 6;114(23):E4602-E4611
pubmed: 28533395
Bioinformatics. 2014 May 1;30(9):1312-3
pubmed: 24451623
Microbiome. 2018 Aug 9;6(1):140
pubmed: 30092815
Sci Rep. 2014 Dec 19;4:7479
pubmed: 25524763
Bioresour Technol. 2019 May;279:385-392
pubmed: 30685132
Front Microbiol. 2016 Jun 17;7:919
pubmed: 27379049
Microbiology (Reading). 2004 Nov;150(Pt 11):3527-3546
pubmed: 15528644
Bioinformatics. 2010 Oct 1;26(19):2460-1
pubmed: 20709691
Nat Microbiol. 2019 Apr;4(4):595-602
pubmed: 30833728
Bioinformatics. 2010 Sep 15;26(18):2342-4
pubmed: 20628077
Annu Rev Microbiol. 2013;67:437-57
pubmed: 23808334
FEMS Microbiol Ecol. 2021 Jan 26;97(2):
pubmed: 33501490
Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1:4516-22
pubmed: 20534432
ISME J. 2020 Nov;14(11):2851-2861
pubmed: 32887944
Nucleic Acids Res. 2021 Jul 2;49(W1):W293-W296
pubmed: 33885785
3 Biotech. 2017 Jun;7(2):118
pubmed: 28567630
FEMS Microbiol Ecol. 2007 Jun;60(3):456-66
pubmed: 17386034
Nat Biotechnol. 2020 Sep;38(9):1079-1086
pubmed: 32341564
Environ Microbiol. 2017 Jun;19(6):2334-2347
pubmed: 28276174
J Exp Bot. 2011 Feb;62(4):1467-82
pubmed: 21282329
Curr Microbiol. 2018 Sep;75(9):1147-1155
pubmed: 29766233
Microorganisms. 2015 Nov 04;3(4):792-808
pubmed: 27682117
Int Microbiol. 2015 Dec;18(4):217-23
pubmed: 27611674
Nat Commun. 2018 Jul 19;9(1):2832
pubmed: 30026532
Sci Rep. 2018 Sep 20;8(1):14155
pubmed: 30237444
Appl Environ Microbiol. 2009 Jul;75(13):4565-72
pubmed: 19429553
J Bacteriol. 2002 Apr;184(7):1952-7
pubmed: 11889103
Science. 1967 Nov;158(3804):1012-9
pubmed: 4861476
Mol Gen Mikrobiol Virusol. 2005;(3):29-34
pubmed: 16173396
Nat Protoc. 2020 Mar;15(3):799-821
pubmed: 31942082
Front Microbiol. 2020 Jul 14;11:1625
pubmed: 32760379
Nat Methods. 2015 Feb;12(2):115-21
pubmed: 25633503
Cell. 2018 Mar 8;172(6):1181-1197
pubmed: 29522741
Trends Microbiol. 2011 Dec;19(12):580-7
pubmed: 22018741
Sci Total Environ. 2021 Jul 15;778:146085
pubmed: 33714092
Extremophiles. 2014 Jul;18(4):763-78
pubmed: 24903703
Curr Protoc Bioinformatics. 2019 Dec;68(1):e86
pubmed: 31756036
BMC Bioinformatics. 2010 Jul 23;11:395
pubmed: 20650010
BMC Bioinformatics. 2010 Oct 30;11:538
pubmed: 21034504