RNA double strand hybridization measured at the single molecule level.
Diffusion coefficient
Dissociation constant
FCS
Fluorescence correlation spectroscopy
RNA
Journal
Analytical biochemistry
ISSN: 1096-0309
Titre abrégé: Anal Biochem
Pays: United States
ID NLM: 0370535
Informations de publication
Date de publication:
01 01 2023
01 01 2023
Historique:
received:
19
09
2022
revised:
16
10
2022
accepted:
17
10
2022
pubmed:
8
11
2022
medline:
23
11
2022
entrez:
7
11
2022
Statut:
ppublish
Résumé
RNA double strand hybridization is a hallmark for gene expression regulation. In this function, single stranded regulatory RNA forms Watson-Crick base pairs with complementary messenger RNA. In the presented work the dissociation constants of complementary equally sized RNA single strands were measured at the single molecule level applying fluorescence correlation spectroscopy (FCS). Dissociation constants of 3.2 nM, 1.4 nM and 1.0 nM were determined for 26 bp, 41 bp and 54 bp dsRNA, respectively. The translational diffusion coefficients of RNA, measured at infinite dilution, could be accurately predicted applying the model D = 4.58 × 10
Identifiants
pubmed: 36341770
pii: S0003-2697(22)00419-5
doi: 10.1016/j.ab.2022.114959
pii:
doi:
Substances chimiques
RNA, Double-Stranded
0
RNA, Messenger
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
114959Informations de copyright
Copyright © 2022 Elsevier Inc. All rights reserved.